Human papillomavirus type 16
Identification of the E5 open reading frame of human papillomavirus type 16.
... 1071 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Sequencing of the E5 open reading frame ORF of human papillomavirus type 16 revealed an additional nucleotide, a th[r] ...
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Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope.
... three type-specific and conformationally dependent monoclonal antibodies, ...pseudotype human papillomavirus type 16 (HPV16) virions in ...
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Membrane Orientation of the Human Papillomavirus Type 16 E5 Oncoprotein
... of human papillomavirus type 16 is a small, hydrophobic protein that localizes predomi- nantly to membranes of the endoplasmic reticulum ...and human ectocervical cells, the C terminus ...
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Characterization of a Major Neutralizing Epitope on Human Papillomavirus Type 16 L1
... Persistent infection with human papillomavirus type 16 (HPV-16) is strongly associated with the develop- ment of cervical cancer. Neutralizing epitopes present on the major coat ...
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Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
... 962 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We cloned and analyzed the integrated human papillomavirus type 16 HPV-16 genomes that are present in the human cerv[r] ...
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Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein.
... In this study, a series of site-specific mutations of the human papillomavirus type 16 E7 gene product were constructed and assessed for their effects on intracellular protein stability,[r] ...
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Effect of human papillomavirus type 16 oncogenes on MAP kinase activity.
... cell type which can be readily trans- formed by each of the HPV-16 oncogenes individually, we chose to use a cell type which was already fully transformed to provide a more uniform background to ...
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Human Papillomavirus Type 16 E7 Oncoprotein Represses Transcription of Human Fibronectin
... The inhibitory effect of HPV upon the hFN promoter was further confirmed when the luciferase activity of pFN-Luc was assayed in several HPV-positive or -negative human tumor cell lines (Tables 1 and 2). A ...
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Low-Dose Adenovirus Vaccine Encoding Chimeric Hepatitis B Virus Surface Antigen-Human Papillomavirus Type 16 E7 Proteins Induces Enhanced E7-Specific Antibody and Cytotoxic T-Cell Responses
... ELISA. Enzyme-linked immunosorbent assay (ELISA) plates were coated overnight with 1 g/ml of either commercially available recombinant HBsAg(S) (Engerix-B), His-tagged E7 protein, -galactosidase (Sigma), or adenovirus ...
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Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16.
... Thus, the BPV and the HPV16 E5 genes can both induce tumorigenic transformation of cultured murine epidermal keratinocytes, but only the viruses carrying the BPV E5 gene display signific[r] ...
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A point mutational analysis of human papillomavirus type 16 E7 protein.
... It is evident from these studies that mutants which destroy both transforming and trans-activation activities p24GLY and mutants which are transformation competent but express no or very[r] ...
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Antiviral activity of Bifidobacterium adolescentisSPM1005-A on human papillomavirus type 16
... Lactic acid bacteria (LAB) are widely used and gener- ally recognized as safe organisms for animal and human applications. They produce antimicrobial substances such as organic acids, hydrogen peroxide, diacetyl ...
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Human Papillomavirus Type 16 E5 Protein as a Therapeutic Target
... the papillomavirus major capsid protein L1 is over-expressed in mammalian, insect, yeast or bacterial cells, capso- meres are spontaneously assembled to form VLPs which are devoid of the oncogenic viral ...animal ...
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Human Papillomavirus Type 16 E1∧E4 Contributes to Multiple Facets of the Papillomavirus Life Cycle
... Western blotting. Cell populations harboring the wild-type or E4 mutant HPV16 genomes were harvested by trypsin digestion, pelleted, resuspended in protein lysis buffer (50 mM Tris [pH 7.4], 150 ml NaCl, 1 mM ...
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TopBP1 Regulates Human Papillomavirus Type 16 E2 Interaction with Chromatin
... The results shown in Fig. 1 and 2 are apparently contradic- tory; in Fig. 1 there seems to be more soluble E2 in the absence of TopBP1, while in Fig. 2 there is clearly less. However, this can easily be explained if the ...
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Human Papillomavirus Type 16 E7 Oncoprotein Associates with E2F6
... promoter constructs reflected the activity of endogenous E2F6-responsive promoters. Generally, it is challenging to de- termine the effects of a particular E2F on gene expression because of the fact that multiple E2Fs ...
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Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA.
... Extensive rearrangements of HPV16 sequences occur in HPV16-transfected human cells; the presence of bands greater than 7.9-kbp in the BamHI-digested DNA from HPV16-transfected human cell[r] ...
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Mutational analysis of human papillomavirus type 16 E7 functions.
... Whereas the immortalizing function transformation in conjunction with activated ras for primary rat cells paralleled the transforming function for rat 3Y1 cells, the transformation funct[r] ...
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Functional Mapping of the Human Papillomavirus Type 16 E1 Cistron
... dividual contribution of endogenously expressed E1 and E2 gene products to initial plasmid amplification, we refined our complementation assay by applying a reductionist approach in which each plasmid species provides a ...
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Papilloma formation in human foreskin xenografts after inoculation of human papillomavirus type 16 DNA.
... the human immune responses to HPVs (3), since allografts of human lymphocytes and other components of the immune system can function in scid mice (24, ...with human immunodeficiency virus infection, ...
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