IFI16 protein
Regulatory Interaction between the Cellular Restriction Factor IFI16 and Viral pp65 (pUL83) Modulates Viral Gene Expression and IFI16 Protein Stability
... pp65, IFI16, nuclear TATA-bind- ing protein (TBP), and cytoplasmic ␣ -tubulin ...the IFI16 protein was expressed at much lower levels in the nuclei of v65Stop-infected cells, starting at 96 ...
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Human Cytomegalovirus pUL83 Stimulates Activity of the Viral Immediate-Early Promoter through Its Interaction with the Cellular IFI16 Protein
... (A) IFI16 and pUL83 were partially colocalized within infected ...and IFI16 to test for ...(B) IFI16 coprecipitates with antibody to ...of IFI16 in those complexes was determined by Western ...
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Interferon gamma inducible protein 16 in primary Sjögren’s syndrome: a novel player in disease pathogenesis?
... A capture enzyme-linked immunosorbent assay (ELISA) was employed for determination of circulating extracellu- lar IFI16 protein following a procedure detailed elsewhere [15]. Briefly, polystyrene micro-well ...
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Mechanisms of Host IFI16, PML, and Daxx Protein Restriction of Herpes Simplex Virus 1 Replication
... of IFI16 in HFFs increases replication of an HSV-1 ICP0-null ...of IFI16 in human foreskin fibroblast (HFF) cells by use of siRNAs increased replication of ICP0-null viruses ...HFF IFI16 knockout ...
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The Nuclear DNA Sensor IFI16 Acts as a Restriction Factor for Human Papillomavirus Replication through Epigenetic Modifications of the Viral Promoters
... The IFI16 protein is a restriction factor of HPV18 replication in NIKS ...For IFI16 silencing, these cells were transfected with either pools of siRNAs targeting IFI16 or an siRNA control and ...
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Relative Contributions of Herpes Simplex Virus 1 ICP0 and vhs to Loss of Cellular IFI16 Vary in Different Human Cell Types
... ICP0 protein is an E3 ubiquitin ligase that promotes the degradation of several host cell pro- ...of IFI16 in infected cells, but one study reported that ICP0 was not necessary or sufficient for loss of ...
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Innate Nuclear Sensor IFI16 Translocates into the Cytoplasm during the Early Stage of In Vitro Human Cytomegalovirus Infection and Is Entrapped in the Egressing Virions during the Late Stage
... that IFI16 is incorporated into the maturing virion ...of IFI16, but not p53, in the viral protein ...of IFI16 into purified virions, and in particular in the outer layer of the tegument in ...
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Interferon γ inducible protein 16 (IFI16) is required for the maintenance of Epstein Barr virus latency
... in IFI16 pro- tein levels, especially compared to Akata cells treated with PAA alone ...in IFI16 observed in these studies is a result of lytic cells undergoing apoptosis in the presence of PAA ...ing ...
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IFI16 is involved in HBV-associated acute-on-chronic liver failure inflammation
... the IFI16 protein levels in only a small number of patients from a single ...of IFI16 in the complete progression of HBV ...intrahepatic IFI16 expression in patients of F4 was significantly ...
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Nuclear Innate Immune DNA Sensor IFI16 Is Degraded during Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus (KSHV): Role of IFI16 in Maintenance of KSHV Latency
... gamma-inducible protein 16) recognizes nuclear episomal herpesvirus (Kaposi’s sarcoma-associated herpesvi- rus [KSHV], Epstein-Barr virus [EBV], and herpes simplex virus 1 [HSV-1]) genomes and induces the ...
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Andreeva, Liudmila (2018): Cytosolic DNA sensing via cGAS: long, U-shaped and other DNA ligands and cellular co-factors. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Detection of virus-derived nucleic acids plays a central role in the initiation of antiviral immunity. Nucleic acid recognition by PRRs results in the secretion of type I interferon (IFN) cytokines and IFN-stimulated ...
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Non canonical Activation of the DNA Sensing Adaptor STING by ATM and IFI16 Mediates NF κB Signaling after Nuclear DNA Damage
... Both IFI16 and p53 assemble on STING soon after etoposide treatment, and interaction between p53 and IFI16 has been shown to be direct (Liao et ...Using IFI16-deficient HaCaT cells, we observed that ...
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IFI16 is Required for an Oligodeoxynucleotide with CCT Repeats to Induce Type I Interferon Production in U937 Cells
... The oligodeoxynucleotide with CCT repeats (CCT ODN) is a human microsatellite DNA sequence-based immunosuppressive ODN and displayed selectively negative regulatory role on TLR9 activation [1-3]. It can restrain TLR9 ...
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The Viral Ubiquitin Ligase ICP0 Is neither Sufficient nor Necessary for Degradation of the Cellular DNA Sensor IFI16 during Herpes Simplex Virus 1 Infection
... fluorescent protein (EYFP)- linked ICP4, and viruses expressing ICP0 mutant proteins FXE ( ⌬ RING), D13 (with mutations of residues 633 to 680 [ ⌬ 633-680]), and E52X ( ⌬ 594-775) have been described previously ...
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Viral DNA Sensors IFI16 and Cyclic GMP AMP Synthase Possess Distinct Functions in Regulating Viral Gene Expression, Immune Defenses, and Apoptotic Responses during Herpesvirus Infection
... of IFI16 to nuclear peripheral foci upon her- pesvirus infections and its well-characterized binding to viral DNA (6) suggest that these foci may be sites of viral DNA deposi- ...endogenous IFI16 in newly ...
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The impact of sex and species of cells in herpes simplex virus infection
... subsequent protein levels of cGAS (Su and Zheng, ...dsRNA-activated protein kinase (PKR), ribonuclease L (RNase L) and JAK1 due to its ribonuclease activity (Murphy et ...vhs protein is involved in ...
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The molecular determinants and consequences of recombination in the evolution of human enteroviruses
... RdRp always switched from pT7Rep3-L in either the small remaining region of PV3 VP1 (of which only 84 nts were present) or the 3’ terminus of luciferase, but never from the 2A region. These subgenomic replicons were ...
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Protein-protein interactions
... The docking of one protein with another is a complex process that involves two basic types of complementarity; geometric and chemical. The majority of docking algorithms are based upon methods of evaluating and ...
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Algorithmic approaches to protein-protein interaction site prediction
... data, protein structural datasets are non-standardized and virtually all structure-based predictors have varying criteria for processing and fil- tering this ...query protein, whether by structural, ...
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A study on the failure of transport protein
... transport protein is misfolded as a mutant, like prion protein, its binding angle to the carrier (substrate) of the side-chain rotamers is changed and its carrying capacity is ...particular protein, ...
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