Implications for adeno-associated viral vector design
The Impact of Peptide Insertions on Adeno-Associated Viral Vector Fate
... the vector dose in case of the HSPG- non-binder mutants A2 and ...Second, vector uncoating could have been impaired in case of proteoglycan- dependently internalized vectors, and third, vector ...
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Vector uncoating limits adeno-associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity
... associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity Axel Rossi 1,2 , Léa Dupaty 3 , Ludovic Aillot 1,8 , Liang Zhang 4 , Célia Gallien 1 , Michael ...
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Adeno-Associated Viral Vector-Driven Expression of Coagulation Proteins for Treatment of Hemophilias and Cancer
... efficacious vector doses in order to prevent anti-AAV capsid CD8 + cytotoxic T cells from destroying the transduced hepatocytes and resulting in loss of transgene ...that vector doses for HB can be ...
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Incorporation of Antigens into Viral Capsids Augments Immunogenicity of Adeno-Associated Virus Vector-Based Vaccines
... FIG 1 Western blot of AAV preparations. Four different AAV vectors (Ag85A-AAV:Ag85A, Ag85A-AAV:GFP, AAV:Ag85A, and AAV:GFP) were assayed for induction of humoral immune responses. Equal genomic particles of each ...
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Adeno-associated virus vector integration junctions.
... shuttle vector, and integrated proviruses containing flanking human DNA were recovered as bacterial plasmids for further ...the vector terminal repeats with no apparent site ...intact. Vector ...
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Vector design influences hepatic genotoxicity after adeno associated virus gene therapy
... and vector integration profiles play import roles in determining whether AAV integrations manifest in genotoxicity and highlight the difficulties of relying heavily upon animal toxicology studies to predict human ...
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Changing Viral Tropism Using Immunoliposomes Alters the Stability of Gene Expression: Implications for Viral Vector Design
... reason viral particles, when deliv- ered into a cell using immunovirosomes or virosomes, are unable to enhance the integration of the virally derived DNA into the genome is not ...
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In vivo model of adeno-associated virus vector persistence and rescue.
... Study design for rescue of AAV-CFTR ...case viral cultures of BAL and nasal lavage fluid were performed prior to Ad2HR405 infection and at 3, 7, 14, and 21 days after ...
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Recent advances in recombinant adeno-associated virus vector production
... rAAV vector produc- simplified method for generating rAAV based on stable tion was the development of helper virus-free packaging HeLa cell lines containing integrated copies of the AAV systems that use a DNA ...
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Adeno associated virus: from defective virus to effective vector
... the viral encoding regions, rAAV design can follow the whole- gene-removal or "gutless" vector rational of, for instance, retrovirus-based vectors in the sense that the cis-acting ele- ments involved ...
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Influence of the SUMOylation pathway on adeno-associated virus vector transduction
... non-degraded viral particles enter the nucleus and are uncoated to release the AAV genome, which is converted to double-stranded (ds) DNA that is used for the expression of the encoded proteins through (Grieger ...
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Creation of a cardiotropic adeno associated virus: the story of viral directed evolution
... One classic method to modify the tropism of a viral vector was to insert a ligand on the virus surface to tar- get the specific cell type. Initially, the targeting ligand was isolated via an indirect ...
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Improving safety and establishing episomal maintenance of Adeno-associated viral vectors
... AAV vector transduction is increased in Ku80-defective ...AAV vector transduction. 177 Furthermore, AAV vector transduction was increased in vitro upon administration of drugs which induce transient ...
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Structure of Adeno-Associated Virus Serotype 8, a Gene Therapy Vector
... Here we report the crystal structure of the AAV8 viral capsid determined to 2.6-Å resolution. The VP topology is very similar to those of previously reported AAV structures, but with variations in regions ...
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Recombinant Adeno-Associated Viral Vectors Are Deficient in Provoking a DNA Damage Response
... p5GFP-AAV2 vector is therefore able to induce a DNA damage ...p5GFP-AAV2 vector led to phospho- RPA32 staining, although this was not within clear foci, as seen with WT ...rAAV2 vector containing the ...
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Host Cell DNA Repair Pathways in Adeno-Associated Viral Genome Processing
... of cells was infected when scAAVGFP-cd reagents were tested. In addition, in order to measure only expression due to circularization of monomeric genomes and not from concate- merized genomes, we used a multiplicity of ...
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Adeno-Associated Virus Vector Genomes Persist as Episomal Chromatin in Primate Muscle
... episomal viral forms were assimilated into ...AAV vector-specific WPRE-pA probe (prior to the cmEPO probe), a nucleosomal ladder was observed in Mac 1, the injected primate, but not in Mac C2, the ...
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Adeno-associated virus as a gene therapy vector: strategies to neutralize the neutralizing antibodies
... AAV viral vectors Recent studies have shown that gene therapy has stunning results for some genetic disorders, including lipoprotein lipase ...applications, viral vectors should have sev- eral features, ...
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Optimization of Recombinant Adeno-Associated Virus (aav) Vector Production in Saccharomyces Cerevisiae
... industrial vector production: Adenovirus (AdV), Herpesvirus (HSV) and Baculovirus (BV) complementation ...challenges associated with the manufacturing ...during vector production refers to the ...
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Characterization of Wild-Type Adeno-Associated Virus Type 2-Like Particles Generated during Recombinant Viral Vector Production and Strategies for Their Elimination
... recombinant vector stocks were not exactly like the wt AAV genome because of differences in the patterns of hybridization following digestion with various restriction endonucleases and Southern blot ...vCMVp-lacZ ...
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