Induced-Pluripotent Stem Cells
Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer
... Induced pluripotent stem cells (iPSCs) share similar characteristics with ESCs of indefinite in vitro growth, and may therefore serve as a useful tool for the targeted genetic modification of ...
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Generation of disease specific induced pluripotent stem cells from patients with rheumatoid arthritis and osteoarthritis
... Insights into the pathophysiology of RA have been derived largely from animal models such as collagen-induced arth- ritis, collagen antibody-induced arthritis, IL-1RaKO mice, and KBX/N serum transfer mice. ...
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Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte like cells
... (DE) cells. These cells correspond to the common pro- genitor from which the liver, lung, pancreas, alimentary tract and thyroid ...DE cells are differentiated into FP cells (stage 2, days ...
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Derivation of induced pluripotent stem cells from orangutan skin fibroblasts
... In addition to the aforementioned conservation efforts, orangutans have also been studied because of their com- plex behaviors [22] and their phylogenetic relationships with humans and the African great apes [23]. While ...
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A SY Stematic approach towards understanding stem cell biology
... various stem cell research ...how stem cells make ‘ decisions ’ during early development and how stem cells function in the ...embryonic stem cells (ESCs), induced ...
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The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells
... Induced pluripotent stem cells (iPS) have become crucial in medicine and ...cancer stem cells (CSCs) and a propensity to form ...normal cells) based on the expression ...
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Temporal specification of blood progenitors from mouse embryonic stem cells and induced pluripotent stem cells
... embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to generate a broad range of differentiated progeny in culture provides a powerful model system for ...
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OCT4 activity during conversion of human intermediately reprogrammed stem cells to iPSCs through mesenchymal epithelial transition
... Human induced pluripotent stem cells (iPSCs), first generated by the ectopic co-expression of four reprogramming factors, octamer- binding protein 4 (Oct4 also known as Pou5f1), SRY-box ...
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Single cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage
... progenitor cells (fNPCs) during the process of reprogramming to induced pluripotent stem cells (iPSCs) and, in reverse, the differentiation of iPSCs towards the neural ...of ...
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iPSC derived β cells model diabetes due to glucokinase deficiency
... whether stem cell–derived β cells recapitulate molecular-physiological phenotypes of a diabetic subject, we generated induced pluripotent stem cells (iPSCs) from subjects with ...
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Programming and reprogramming the brain: a meeting of minds in neural fate
... that cells can naturally be reprogrammed or can be forced to do so, most famously by using one or several transcription ...When induced experimentally, the identity of the chosen transcription factor(s) ...
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Novel approaches using human induced pluripotent stem cells and microRNAs in the development of relevant human hepatocyte models for drug-induced liver injury
... Despite the development of various hepatic models for use in screening for adverse effects of new drugs and to aid mechanistic understanding of hepatotoxicity, drug-induced liver injury (DILI) in humans remains a ...
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BMP4 directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell state
... human pluripotent stem cells (hPSCs), including embryonic (hESCs) and induced pluripotent stem cells (hiPSCs), as useful models for trophoblast differentiation; ...
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Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond
... human induced pluripotent stem cells (iPSCs) have emerged as an attractive platform for overcoming these conventional limitations of animal models for disease modeling and drug discovery (Shi ...
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The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
... when pluripotent stem cells are induced to differentiate, and that glutathione reductase is an important enzyme involved in the cellular response to oxidative stress, we decided to study Gsr ...
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Inducible Gata1 suppression expands megakaryocyte erythroid progenitors from embryonic stem cells
... with stem cell factor (SCF), thrombopoi- etin (TPO), and dox (Supplemental Figure ...in cells expressing the corresponding shRNAs (Supplemental Figure ...ES cells ceased after about 2 weeks, while ...
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Hurdles to clinical translation of human induced pluripotent stem cells
... Poor stem cell survival and engraftment after delivery is partly due to cellular rejection triggered by the host’s immune response (37). The lack of an effective method of inducing immune tolerance to maintain ...
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Molecular mechanisms of pluripotency and reprogramming
... ICM cells and ES cells and is an essential factor in most reprogramming experiments ...ES cells showed that Oct4 binds to as many as 92 ...ES cells, but some are ubiquitously expressed in all ...
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Temporal epigenetic modifications differentially regulate ES cell like colony formation and maturation
... lentivirus-infected cells had higher levels of Oct4 expression than that observed in control, VPA- or 5-aza-treated non-infected, and non-treated Oct4- lentivirus infected cultures (Figure ...embryonic stem ...
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Ten years of induced pluripotency: from basic mechanisms to therapeutic applications
... The CiRA/ISSCR symposium ‘ Pluripotency: From Basic Science to Therapeutic Applications ’ brought together an inspiring mix of speakers (Fig. 2) and covered a broad range of topics. Despite the diversity, the underlying ...
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