Inducible expression
A novel pair of inducible expression vectors for use in Methylobacterium extorquens
... Here we describe the construction of two IncP-based, inducible expression vectors for use in M. extorquens, and possibly numerous other proteobacteria with minor modification. The novelty of these vectors ...
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Inducible expression of cancer-testis antigens in human prostate cancer
... restricted expression and immunogenicity ...heterogeneous expression of CTAs in a wide variety of cancers, including primary PC ...induce expression of immunologically potentially relevant CTAs in ...
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Inducible Expression of Human Gamma Interferon
... As we mentioned in the introduction, IFN-γ is supposed to be a cytotoxic for cell proliferationat high concentration. Therefore, for cloning and expression IFN-γ, we used the cumate-regulatable expression ...
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Human immunodeficiency virus vectors for inducible expression of foreign genes.
... To determine whether H5 HA expression could be detected following infection with GB112, virus was produced via COS cell transfection as described above by using GB111 or GB112 as the vec[r] ...
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6008.pdf
... Here we experimentally test the Complexity Hypothesis. We measure the fitness cost of a set of proteins transferred into E. coli from Vibrio cholerae and Staphylococcus aureus, which have diverged from E. coli by ...
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Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis
... the expression cassette, AscI-NotI restriction sites were engineered just downstream of nucA ...For inducible expression of the scaffolds, we replaced the P59 promoter with P nisA from ...
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Stabilization of histone demethylase PHF8 by USP7 promotes breast carcinogenesis
... (Dox-inducible) expression of stably integrated ...Dox-inducible expression of FLAG-PHF8 were collected and subjected to affinity purification using an anti-FLAG affinity ...
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Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans
... gene expression in ...gene expression in ...gene expression, the caGFP reporter gene was placed under the control of a Tet-dependent promoter, obtained by fusing a minimal promoter from ...gene ...
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Construction of a doxycycline inducible adipogenic lentiviral expression system
... gene expression by binding to the Tet Operator (tetO) within the tetracycline response element (TRE) to either switch off expression (tet-Off) or switch on expression ...On expression system ...
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An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis
... gene expression in an ...gene expression is an alternative when heterologous expression has failed ...appropriate expression vectors for ...of expression systems for Enterococcus are ...
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Mohr, Hermine (2012): Cytomegalovirus replicon-based regulation of gene expression in vitro and in vivo. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... novel inducible expression system, namely the replicon vector, which is induced by infection with wt ...Transgene expression of the replicon vector was reliably silenced in all tested cell ...other ...
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Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis
... gene expression can be tightly controlled via the addition of the appropriate concentration of ...agmatine-inducible expression of the gene encoding ...ACE expression system could be a very ...
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Original Article High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats
... Hypoxia increases the expression of inducible nitric oxide synthase (iNOS). Nitric oxide (NO) generated from NOS is also involved in many physiological and pathological processes, including hypoxic ...
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An inducible transgene expression system for zebrafish and chick
... conditional expression system amenable to both spatial and temporal ...transgene expression by drug withdrawal (Knopf et ...different inducible GAL4 systems have been used in Drosophila (RU486) ...
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Differential expression of three T lymphocyte activating CXC chemokines by human atheroma associated cells
... differential expression of the 3 IFN-g–inducible CXC chemokines — IFN-inducible protein 10 (IP-10), monokine induced by IFN-g (Mig), and IFN-inducible T-cell a chemoattractant (I- TAC) — by ...
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TLR2 signal influences the iNOS/NO responses and worm development in C57BL/6J mice infected with Clonorchis sinensis
... gene expression, which led to different infection outcomes ...iNOS expression dropped rapidly in TLR2 mutant C57BL/6 mice at 120 dpi, and dead worms were generally observed in the histological sec- ...
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Development of diabetes mellitus in aging transgenic mice following suppression of pancreatic homeoprotein IDX 1
... IDX-1 expression in the pathogenesis of diabetes, we developed a model for the inducible impairment of IDX-1 expression in pancreatic β cells in vivo by engineering an antisense ribozyme specific for ...
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Developing tTA Transgenic Rats for Inducible and Reversible Gene Expression
... conditional expression of desired genes in rats, we generated several lines of the transgenic rats carrying the tetracycline-controlled transactivator (tTA) ...widespread expression of tTA in various ...
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Androgen receptor expression in human prostate cancer cell lines
... COS-1 cells were transiently transfected with the four AR expression vectors (pcDNA-AR, pSVARo, pCMVhAR, and pTRE-AR). Total RNA, isolated 48 hours after transfection, was reverse transcribed into cDNA which was ...
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Evolution of Steroid-Inducible RP2 mRNA Expression in the Mouse Kidney
... T o determine if this response is conserved within the Mus genus, we examined kid- ney RP2 mRNA levels in normal and testosterone- treated females from the other species.[r] ...
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