L. interrogans
Isolation of L interrogans Serovar Pomona in 14 Human Cases and an African Lion, All with Chronic Leptospirosis
... Depending on its time of evolution, leptospirosis presents two phases: acute and chronic. The chronic leptospirosis is widely accepted in animals and, until recently, this was denied in humans. Since the 1990s, we have ...
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Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays
... with L. interrogans serovar portlandvere, being dif- ferentiated from serovar canicola by the presence of a single restriction fragment approximately 15 kb in size ...
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Multilocus Sequence Analysis for Typing Leptospira interrogans and Leptospira kirschneri
... Copenhageni strain Fiocruz L1-130, and L. interrogans serovar Lai strain 56601 were used to construct a phylogenetic tree (Fig. 1). Sequenced DNA fragments were concatenated in the following order to ...
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Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species
... Several molecular tools that have been so far described for the characterization of Leptospira are associated with sev- eral drawbacks. Methods like PFGE, RFLP, and REA need large quantity of purified DNA, present ...
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Development of an Enzyme-Linked Immunosorbent Assay Using a Recombinant LigA Fragment Comprising Repeat Domains 4 to 7.5 as an Antigen for Diagnosis of Equine Leptospirosis
... Cloning, expression, and purification of proteins. Primers for the gene segments encoding these truncated proteins are listed in Table 1. The underlined nucleotides indicate the restriction sites added to facilitate ...
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Development of transcriptional fusions to assess Leptospira interrogans promoter activity
... Although L. interrogans is a pathogen known to infect the mammalian host through active penetration, there is uncertainty over the existence of an intracellular phase during this ...alters L. ...
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Protective Immunity and Reduced Renal Colonization Induced by Vaccines Containing Recombinant Leptospira interrogans Outer Membrane Proteins and Flagellin Adjuvant
... ing to 100-fold the 50% lethal dose (LD 50 ) calculated by the method of Reed and Muench (38). Animals surviving after 21 days were considered protected. Protection was calculated as the percentage of animals that were ...
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Roczek, Alexandra (2008): Entwicklung einer quantitativen PCR zum Nachweis von DNA pathogener Leptospiren in Glaskörper- und Kammerwasserproben von Pferden. Dissertation, LMU München: Tierärztliche Fakultät
... von L. interrogans für die Inhibitionskontrolle ausgewählt wurde, war es erstaunlich leicht geeignete Primer zu ...Genospezies L. interrogans ein spezifisches PCR-Produkt ...
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High Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing
... Leptospira interrogans, which is the main agent of leptospirosis ...of L. interrogans serogroups Icterohaemorrhagiae, Australis, Canic- ola, and Grippotyphosa, which are the most prevalent serogroups ...
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Leptospira seroprevalence and associations between seropositivity, clinical disease and host factors in horses
... Serological evidence of leptospiral infection is common in horses. Predominant serovars reported are L. interrogans sv Pomona, L. interrogans sv Bratislava, L. interrogans sv ...
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A Conserved Region of Leptospiral Immunoglobulin-Like A and B Proteins as a DNA Vaccine Elicits a Prophylactic Immune Response against Leptospirosis
... Leptospira interrogans serovar Canicola as DNA vaccines using the pTARGET mammalian expression ...with L. interrogans serovar Copenhageni strain Spool via the intraperitoneal ...
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Vaccination with Leptospiral Outer Membrane Lipoprotein LipL32 Reduces Kidney Invasion of Leptospira interrogans Serovar Canicola in Hamsters
... Histology. Samples from liver, spleen, and kidney were collected from all hamsters at postmortem examination and fixed in 10% (vol/vol) buff- ered formalin. The tissue samples were routinely processed and embed- ded in ...
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Identification of Variable Number Tandem Repeat Loci in Leptospira interrogans Sensu Stricto
... from L. interrogans have been isolated in Asia (2), mainly in southeast Asia (Table ...among L. interrogans clinical strains, therefore demonstrating the usefulness of this PCR- based method ...
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Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia
... for L. interrogans Sensu Stricto, this research highlighted the value of using VNTR as a typing scheme but was limited to the identification of serovar by compar- ing the size of the repeat to that of known ...
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Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L borgpetersenii, and L kirschneri) with species specific DNA probes produced by arbitrarily primed PCR
... The purpose of this study was to develop a strategy for producing nonradioactive DNA probes able to discriminate among the pathogenic Leptospira species. DNA-DNA hybrid- ization data demonstrated the high level of ...
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Use of Luminescent Leptospira interrogans for Enumeration in Biological Assays
... Despite intensive research into the pathogenic mechanisms of L. interrogans, progress has been limited due to the practi- calities of working with these bacteria. One significant techni- cal difficulty is ...
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Determination of Leptospira borgpetersenii serovar Javanica and Leptospira interrogans serovar Bataviae as the persistent Leptospira serovars circulating in the urban rat populations in Peninsular Malaysia
... namely; L. borgpetersenii serogroup Javanica (n = 16) and L. interrogans serogroup Bataviae (n = ...populations: L. borgpetersenii serovar Javanica (41 %), and L. interrogans ...
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Identification of New Repetitive Element inLeptospira interrogans Serovar Copenhageni and Its Application to PCR Based Differentiation ofLeptospira Serogroups
... L. interrogans serovar canicola based on the reference strain pattern. Standard serologic methods were used to confirm the serovar status of clinical isolates. Interestingly, the iRep1 PCR found that two ...
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Serological interrelationship of Leptospira serovar and genus specific antigens by enzyme linked immunosorbent assay
... The serological interrelationship of a sonicated antigen POM-S and an alkali-extracted "fraction 4" antigen POM-F4 from Leptospira interrogans serovar pomona, and an ethanol-precipitated[r] ...
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rpoB Gene Analysis as a Novel Strategy for Identification of Spirochetes from the Genera Borrelia,Treponema, and Leptospira
... FIG. 3. Detection of spirochetes from a mixed bacterial suspension using specific primers for the rpoB gene. The PCR assay was performed under the same conditions as described in the legend to Fig. 2, but with ...
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