label-free quantitative proteomics
Label-free quantitative proteomics identifies transforming growth factor β1 (TGF-β1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells
... our label-free quantitative proteomics analysis identified a total of 139 proteins dif- ferentially expressed in rosiglitazone-treated cells com- pared with DMSO-treated cells, among which ...
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Label-free quantitative proteomics of CD133-positive liver cancer stem cells
... spectrometry-based label-free quantitative proteomics developed in the current study can be used to gain the insights into liver cancer stem ...
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Label-Free Quantitative Proteomics of Lysine Acetylome Identifies Substrates of Gcn5 in Magnaporthe oryzae Autophagy and Epigenetic Regulation
... Overall, we infer that Gcn5 may catalyze acetylation on Pk lysine 188, which facilitates its degradation in an autophagy-dependent manner. This observation is consistent with a recent report for cancer cells that ...
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Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics
... Dimethyl sulfoxide (DMSO) has been advocated as a beneficial additive to electrospray solvents for peptide analysis due to the improved ionisation efficiency conferred. Previous reports have shown that the resultant ...
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Label free quantitative proteomics reveals the role of miR-200b in androgen-independent prostate cancer cells
... searching. Label-free quantification was carried out in MaxQuant, as described elsewhere ...spectrometry proteomics data have been deposited to the ProteomeXchange Consor- tium via the PRIDE [1] ...
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Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB)
... date, quantitative proteomic methods have been applied in the study of various can- cer, such as breast cancer, lung cancer, pancreatic cancer and gastric cancer ...
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Label free quantitative proteomics reveals regulation of interferon induced protein with tetratricopeptide repeats 3 (IFIT3) and 5' 3' exoribonuclease 2 (XRN2) during respiratory syncytial virus infection
... the influence of RNA virus infection on the cellular pro- teome, since novel viral proteins are introduced to the cel- lular environment and immediate changes are likely to occur on the posttranslational level. In ...
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Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics
... Several acetylome analyses have been performed to understand the function of acetylation proteins in dif- ferent human cancers [16–18]. To the best of our knowl- edge, there are no reports on large scale analyses of ...
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Comparative Proteomic Analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield
... using label- free quantitative proteomics based on the exponentially modified protein abundance index (emPAI) [13-15] to investigate the molecular and metabolic mechanisms underlying the ...
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Elucidation of the Cellular Interactome of Ebola Virus Nucleoprotein and Identification of Therapeutic Targets
... Table 1: Cellular proteins that have a higher probability of forming protein-protein interactions with EBOV/NP. Shown are candidate proteins identified using the (A) EBOV EGFP-NP, (B) EBOV NP-EGFP fusion proteins and ...
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Application of label free mass spectrometry based proteomics to biomarker discovery
... In contrast to DDA, MS E acquisitions collect alternating low energy MS scans which are also used for quantitation, with elevated energy scans for peptide identification. Assuming a sufficiently fast scan rate, LC-MS E ...
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Quantifying raft proteins in neonatal mouse brain by 'tube-gel' protein digestion label-free shotgun proteomics
... not quantitative, therefore, it is difficult to assess the abundance of a particular protein from the MS data per ...with label-free LC-MS/ MS shotgun proteomics [29,53-57] revealed a ...
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Quantitative proteomics in lung cancer
... progression. Quantitative proteomics provides the relative different protein abundance in normal and cancer patients which offers the information for molecular interactions, signaling pathways, and ...
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Novel mass spectrometry based approaches for the characterisation of systems of biological interest
... in label-free experiments. The recent developments in quantitative proteomics have resulted in an exponential increase in data volume and ...
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A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development
... Label-free proteomics allows for the quantification of peptides using spectral characteristics such as retention time, m/z ratio and peak intensity by comparing the direct mass spectrometric signal ...
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Label-free proteomics identifies Calreticulin and GRP75/Mortalin as peripherally accessible protein biomarkers for spinal muscular atrophy
... would make it much easier to obtain quick, repeated tissue samples for monitoring purposes. Therefore, we next asked whether GRP75/Mortalin and Calreticulin protein could be reliably identified and measured in skin and ...
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Development and application of label free quantitative proteomic methods
... a free N‐terminal group and a C‐terminal lysine or arginine which are both sites where a positive charge can be added through protonation ...up proteomics can also be performed with no initial gel based ...
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Label-free quantitative phosphorylation analysis of human transgelin2 in Jurkat T cells reveals distinct phosphorylation patterns under PKA and PKC activation conditions
... several proteomics studies have suggested transgelin2 as a poten- tial biomarker candidate of tumorigenesis and metastasis, the functional roles of transgelin2 have predominantly been investigated using various ...
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Molecular signatures of sanguinarine in human pancreatic cancer cells: A large scale label-free comparative proteomics approach
... the proteomics changes incurred by sanguinarine treatment in pancreatic cancer ...a label-free nano- ESI ultra high resolution mass spectrometry approach employing Q-Exactive hybrid ...
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A comparative study of analysis methods in quantitative label free proteomics
... Each laboratory performs biological experiments using the instrumentation that they have available, or which they have chosen (from a small or wide range of options) as most appropriate for a given experiment, and the ...
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