Large T-Antigen
Localization of the phosphorylations of polyomavirus large T antigen.
... Hydroxylamine cleavage of large T antigen labeled with [35S]methionine yielded peptides that matched electrophoretically the two phosphorylated fragments.. A third fragment 60K fragment [r] ...
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Activation of CREB/ATF Sites by Polyomavirus Large T Antigen
... Polyomavirus large T antigen (LT) has a direct role in viral replication and a profound effect on cell ...of large T function is related to its effects on tumor suppressors of the ...
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Interaction between Simian Virus 40 Large T Antigen and Insulin Receptor Substrate 1 Is Disrupted by the K1 Mutation, Resulting in the Loss of Large T Antigen-Mediated Phosphorylation of Akt
... The cellular kinase Akt is a key controller of cellular metabolism, growth, and proliferation. Many viruses activate Akt due to its beneficial effects on viral replication. We previously showed that wild-type (WT) simian ...
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... is large T antigen (T-ag), the initiator protein from simian virus 40 ...of T-ag that has ATPase and minimal unwinding activity is the hexamer, pro- cessive unwinding and bidirectional ...
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Characterization of an immortalizing N-terminal domain of polyomavirus large T antigen.
... 668 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary [r] ...
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Polyomavirus Large T Antigen Binds the Transcriptional Coactivator Protein p300
... the T antigens, and in the HPV E7 protein, the status is not as clear with respect to ...SV40 large T antigen (SV40LT) was recently found to bind p300 and its relatives (4, 10), amino acid ...
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J Domain-Independent Regulation of the Rb Family by Polyomavirus Large T Antigen
... polyomavirus large T antigen (LT) to promote cell cycling, to immortalize primary cells, and to block differentiation has been linked to its effects on tumor suppressors of the retinoblastoma ...
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Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen.
... Fusion proteins of large T antigen lacking the first 200 N-terminal amino acids bound specifically to polyomavirus origin DNA; however, deletions beyond this site resulted in unstable pr[r] ...
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Evidence for transmembrane orientation of acylated simian virus 40 large T antigen.
... 541 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In mKSA cells a simian virus 40-transformed BALB/c mouse tumor cell line, plasma membrane-associated large T antigen[r] ...
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Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin.
... To determine whether binding of large T antigen was responsible for the defective replication of these DNAs and, more generally, to show that binding of large T antigen to the origin was[r] ...
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... small-t antigen did not play a role in causing this phenotype came from virus infection ex- periments, as shown in ...of large-T antigen than does WT SV40 ...of large-T ...
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Detection and characterization of multiple forms of simian virus 40 large T antigen.
... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ...
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The Minimum Replication Origin of Merkel Cell Polyomavirus Has a Unique Large T-Antigen Loading Architecture and Requires Small T-Antigen Expression for Optimal Replication
... MCV large T protein (LT). The origin includes a poly(T)-rich tract and eight variably oriented, GAGGC-like pentanucleotide sequences (PS) that serve as LT recognition ...small T protein (sT), ...
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Cyclin-dependent kinase regulation of the replication functions of polyomavirus large T antigen.
... mutant large T Ag by the cdc2-cyclin B kinase ...Py T Ags were capable of being phosphorylated by the CDK complex (upper ...Py T Ag peptides spanning residues 60 to 240 and 24 to 521, ...
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Metabolic turnover of phosphorylation sites in simian virus 40 large T antigen.
... Thus, for all lysates, the total 32P radioactivity incorporated in 88K large T molecules 0 is shown, as well as the 32p radioactivity associated with the N-terminal P-Ser site l, the N-t[r] ...
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High-frequency recombination mediated by polyomavirus large T antigen defective in replication.
... We show here that in another cell line, designated Hy5, polyomavirus large T-Ag can promote different recombination events independently of its replicative function and that recombinatio[r] ...
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The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence.
... To determine whether the inability of these deletion mutants to transform cells was due to the short half-life of the protein within the cell and not to the disruption of a potential tra[r] ...
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Mapping the transcriptional transactivation function of simian virus 40 large T antigen.
... Two mutants with lesions in the DNA-binding domain that were unable to bind to SV40 DNA were completely defective for transactivation of both promoters, while a third mutant with a lesio[r] ...
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Polyomavirus tumor induction in mice: effects of polymorphisms of VP1 and large T antigen.
... 335 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest By testing recombinants between "high tumor" inducing a high incidence of tumors and "low tumor" inducing a low inci[r] ...
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ...
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