Leishmania parasites
Evaluation of a Microculture Method for Isolation of Leishmania Parasites from Cutaneous Lesions of Patients in Peru
... Traditional culture of Leishmania spp. is labor intensive and has poor sensitivity. We evaluated a micro- culture method for the diagnosis of cutaneous leishmaniasis in consecutive patients presenting to the ...
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Identification of a RAC/AKT like gene in Leishmania parasites as a putative therapeutic target in leishmaniasis
... in Leishmania donovani, which showed a signifi- cant similarity with mammalian ...in Leishmania parasites, which was suggested to be phos- phorylated under parasite exposure to stressful conditions, ...
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Quantitative Nucleic Acid Sequence Based Assay as a New Molecular Tool for Detection and Quantification of Leishmania Parasites in Skin Biopsy Samples
... quantifying Leishmania parasites following treatment and showed that per- sistence of parasites in the blood after treatment was associ- ated with a high risk of ...
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Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
... detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical ...detecting Leishmania RNA, was evaluated using ...
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Genomic instability at the locus of sterol C24 methyltransferase promotes amphotericin B resistance in Leishmania parasites
... B-resistant Leishmania mexicana ...in Leishmania genomes, its presence associated with resistance to a major antileishmanial drug, with no evidence of associated fitness costs, is a significant ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely ...of ...
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Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine
... of Leishmania parasites in blood ali- quots used in culture and missing in the aliquots used for DNA extraction; thus there is not enough to be amplified by PCR as the buffy coat is the third most ...
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Ethnomedicines and anti-parasitic activities of Pakistani medicinal plants against Plasmodia and Leishmania parasites
... against Leishmania and Plas- modium ...era, parasites are showing resistance to common allopathic drugs, while on the other side medicinal plants have proven their effec- tiveness as anti-parasitic ...
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Natural transmission of Leishmania infantum through experimentally infected Phlebotomus perniciosus highlights the virulence of Leishmania parasites circulating in the human visceral leishmaniasis outbreak in Madrid, Spain
... Specific anti-L. infantum IgG antibody response gener- ated by the analyzed animals was measured by ELISA as described elsewhere [44]. Soluble Leishmania antigen (SLA) was prepared from L. infantum promastigotes ...
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Specific in vitro cleavage of a Leishmania virus capsid-RNA-dependent RNA polymerase polyprotein by a host cysteine-like protease.
... sucrose-purified Leishmania virus particles isolated from infected ...on Leishmania RNA virus 1-4 transcripts, we have developed an in vitro cleavage assay system to explore the possibility that the fusion ...
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Identification of Leishmania tropica and Leishmania aethiopica by DNA sequencing and the detection of Leishmania RNA Virus
... the Leishmania RNA virus has been shown to be responsible for a hyper-inflammatory ...of parasites in the blood and greater footpad swelling (Ives et ...the Leishmania infection; this suggests that ...
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Bacterial diversity of wild caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis
... dissimilarity measurements, were performed on a matrix encompassing the bacterial OTU relative abundance data pertaining to the four tested conditions that were previously explained. Sequencing methods (considering ...
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Canine leishmaniosis in South America
... of Leishmania parasites to dogs in South America is ...of Leishmania infection to different Lutzomyia species ...to Leishmania infection in dogs, keeping in mind that these concepts cannot be ...
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An integrated pipeline for the development of novel panels of mapped microsatellite markers for Leishmania donovani complex, Leishmania braziliensis and Leishmania major.
... In addition to the development of the micro- satellite extractor programme we have also assessed the use of clinical samples and nested-PCR to avoid the need for culturing in the genotyping of Leishmania ...
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Ability of real time PCR for differential diagnosis of various forms of cutaneous leishmaniasis: a comparative study with histopathology
... of parasites from lesions biopsy samples [10, ...of leishmania parasites within the skin lesions would be important not only for diagnostic purposes, but also for an eventual follow-up of a patient’s ...
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A cross sectional study of the filarial and Leishmania co endemicity in two ecologically distinct settings in Mali
... LST is an indication of previous exposure to Leishmania parasites. LST material is supplied as sterile aqueous 2.5 ml suspensions, with each ml containing 6 × 106 killed L. major promastigotes (strain ...
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Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR
... In the present study, we applied a PPIP-PCR method to identify Leishmania parasites. PPIP- PCR analysis showed that among the positive samples, all isolates produced fragments heavy and light. In this size ...
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Toll like receptor 2 (TLR2) plays a role in controlling cutaneous leishmaniasis in vivo, but does not require activation by parasite lipophosphoglycan
... of Leishmania parasites in vitro, in particular via sensing of lipophosphoglycan (LPG), the major surface glycolipid present on the infect- ive promastigote stage ...
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Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
... 21. Van der Meide W, Guerra J, Schoone G, Farenhorst M, Coelho L, Faber W, et al. Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for ...
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Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq
... intact parasites; this may pose a problem particularly in the chronic phase of CL where pa- rasite levels in skin lesions are very ...detect Leishmania species in clinical cutaneous samples and compared ...
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