Length heterogeneity localised to the intergenic spacer
High-Frequency Intragenomic Heterogeneity of the Ribosomal DNA Intergenic Spacer Region in Trichophyton violaceum
... RFLPs in the rDNA of T. violaceum isolates. Hybridization of an rDNA probe to immobilized EcoRI restriction fragments of genomic DNA identified extensive RFLPs in the rDNA of 32 Taiwanese isolates of T. violaceum (Fig. ...
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Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis
... the length polymorphism of the intergenic spacers lying between 16S and 23S rRNA genes (6) or between tRNA genes (2, 27) is useful for identifying microorganisms to the species ...
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Rapid Identification of Clinically RelevantLegionella spp by Analysis of Transfer DNA Intergenic Spacer Length Polymorphism
... phism by tDNA-ILP assay. We previously reported a similar discrepancy for tDNA-ILP patterns of viridans group strepto- cocci (4). Our results were obtained in 8 h, starting with 72-h-old bacterial growth on solid medium, ...
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Extraordinary Ribosomal Spacer Length Heterogeneity in a Neotyphodium Endophyte Hybrid: Implications for Concerted Evolution
... of length heterogeneity was found in the ribosomal DNA (rDNA) of an asexual hybrid Neotyphodium grass endophyte, isolate ...the length heterogeneity was not found in either of these progenitor ...
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The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
... We next used a bioinformatics tool to further evaluate the distinctiveness among these species taking into ac- count the nucleotide composition, regardless of base order and length. We extracted from the whole ...
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Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species
... fragment length values that are present in the fingerprints of its different isolates, and some also include negative values to distinguish between highly sim- ilar ...
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Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species
... the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus ...in length from 195 to 719 ...
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Dynamic Changes in the Distribution of a Satellite Homologous to Intergenic 26-18S rDNA Spacer in the Evolution of Nicotiana
... showed that inserts contained all kinds of oligomers The A1/A2 subrepeats of the IGS have dispersed into up to pentamers with dimers and trimers being most the genome of section Tomentosae and were then carried abundant. ...
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Species Identification and Strain Differentiation of Dermatophyte Fungi by Analysis of Ribosomal DNA Intergenic Spacer Regions
... indeterminate length of the NTS region was polymorphic in size between ...the length of this fragment varied over the range ...in length to the sum of the sizes of the first two ...
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Molecular analysis of the hot spot region related to length mutations in wheat chloroplast DNAs. I. Nucleotide divergence of genes and intergenic spacer regions located in the hot spot region.
... sequence comparison among closely related species such as wheat and Aegilops is advantageous for clari- fying the nature of nucleotide substitutions in the hypervariable [r] ...
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Comparison of internal transcribed spacers and intergenic spacer regions of five common Iranian sheep bursate nematodes.
... Previous studies have indicated that se- quences of the ITS and IGS regions of rDNA are useful targets to find genetic markers (7-9, 15) for the differentiation between nematode species. In the present study we have ...
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A PCR Based Intergenic Spacer Region Capillary Gel Electrophoresis Typing Method for Identification and Subtyping of Nocardia Species
... Limitations of this study include the relatively small number of less common nocardiae studied. In addition, isolates in our study may have different profiles from those found in other parts of the world. For example, ...
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Identification of Acinetobacter isolates in the A calcoaceticus A baumannii complex by restriction analysis of the 16S 23S rRNA intergenic spacer sequences
... rRNA intergenic spacer ...restriction length polymorphism in the spacer sequences between the 16S and 23S rRNA genes as a method for the identification of isolates in the ...
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Identification of Clinically Relevant Nonhemolytic Streptococci on the Basis of Sequence Analysis of 16S 23S Intergenic Spacer Region and Partial gdh Gene
... A high degree of heterogeneity within the S. anginosus spe- cies has been reported previously (27). It was suggested that the species contains several subspecies or new species. In our study, we included only one ...
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Intra-genomic internal transcribed spacer region sequence heterogeneity and molecular diagnosis in clinical microbiology
... transcribed spacer region; sequencing; heterogeneity; yeast; molecular identification ...and/or intergenic regions in the fungal genome and direct nucleotide sequencing of the purified PCR ...
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Molecular Typing of Papillomatous Digital Dermatitis Associated Treponema Isolates Based on Analysis of 16S 23S Ribosomal DNA Intergenic Spacer Regions
... Interestingly, only sequences of PDD-associated Treponema isolates that are related to T. denticola have been PCR ampli- fied from PDD lesions of cattle in the United Kingdom (4, 14). The availability of the 16S-23S rDNA ...
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Intergenic 16S rRNA gene (rDNA) 23S rDNA sequence length polymorphisms in members of the family Legionellaceae
... L. cincinnatiensis exhibited an apparent single spacer band of approximately 1,250 bp (Fig. 1). The strong intensities of the ethidium bromide-stained PCR products exhibited by L. du- moffii and L. cincinnatiensis ...
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Application and Evaluation of the Interlaboratory Reproducibility of tRNA Intergenic Length Polymorphism Analysis (tDNA PCR) for Identification of StreptococcusSpecies
... Discriminatory power. Most species were easily distinguish- able using tDNA-PCR (Table 6). The closely related species S. bovis, S. alactolyticus, and S. gallolyticus showed resembling but distinctive tDNA fingerprints. ...
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Molecular Assessment of 16S 23S rDNA Internal Transcribed Spacer Length Polymorphism of Aeromonas hydrophila
... In the present investigation, there was one common band (750 bp) which was present in all these isolates of A. hydrophila except the AH21. Several other bands were also amplified and used for construction of the finger- ...
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Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes
... Furthermore, a detailed understanding of the transmission of T. mentagrophytes strains between people and insights into the pathogenic mechanisms of this species will require a means of distinguishing between strains. ...
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