Ligase activity
Depletion of Intracellular Zinc Inhibits the Ubiquitin Ligase Activity of Viral Regulatory Protein ICP0 and Restricts Herpes Simplex Virus 1 Replication in Cell Culture
... ubiquitin ligase activity, cDNAs encoding the RING domains of ICP0-FXE (8) and -RF (22) were transferred into the bacterial expression vector pGEX241 ...
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Role of TRIM5α RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus
... ubiquitin ligase activity of TRIM5 ...ubiquitin ligase activity in vitro, resulting in self-ubiquitylation ...uitin ligase activities of TRIM5 ␣ ...
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Smac/Diablo antagonizes ubiquitin ligase activity of inhibitor of apoptosis proteins
... ligase activity. Our observation that Smac/Diablo inhibits the Ub ligase activities of the mammalian IAPs suggests that IAP-mediated modification of caspases may contribute to their ability to act as ...
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The RING Finger Domain of Varicella-Zoster Virus ORF61p Has E3 Ubiquitin Ligase Activity That Is Essential for Efficient Autoubiquitination and Dispersion of Sp100-Containing Nuclear Bodies
... ubiquitin ligase activity, which is required for autoubiquitination and targeting host proteins for proteasomal degradation, maps to its RING finger domain (1, 7) and, re- cently, a fragment of ORF61p ...
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Latency-Associated Nuclear Antigen E3 Ubiquitin Ligase Activity Impacts Gammaherpesvirus-Driven Germinal Center B Cell Proliferation
... We also assessed mLANA distribution within cells. In mLANA- m4TR episome-containing cells (Fig. 3), mLANA (green) was concentrated into dots along mitotic chromosomes (red) and in the nuclei of interphase cells (overlay ...
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Human BRCA1 BARD1 Ubiquitin ligase activity counters chromatin barriers to DNA resection
... Ub ligase promotes a subset of DNA repair functions attributed to BRCA1 and participates in promoting resection steps after CtIP and MRE11-mediated commitment to ...BRCA1 ligase activity and SMARCAD1 ...
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Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae
... SUMO ligase activity is deficient, exhibits defects in the ex- port of ribosomal ...SUMO ligase activity of Mms21 is required for Smc5/6 complex-mediated DNA repair (Bermudez-Lopez et ...SUMO ...
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NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner
... Nedd4 family might also be Nedd8 liagses. On the other hand, we demonstrated that NEDL2 regulates GDNF/ Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase ...
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The ubiquitin E3 ligase activity of BRCA1 and its biological functions
... E3 ligase activity of BRCA1 immunocomplexes precipitated from IR treated cells when incubated with ubiquitin, E1 and ATP, but not with ...E3 ligase activity was accompanied by dissociation of ...
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Neddylation and CAND1 Independently Stimulate SCF Ubiquitin Ligase Activity in Candida albicans
... SCF (Skp1– cullin/Cdc53–F-box protein) ubiquitin ligases bind substrates via the variable F-box protein and, in conjunction with the RING domain protein Rbx1 and the ubiquitin-conjugating enzyme Ubc3/Cdc34, catalyze ...
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Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus
... coli, uninfected animal cells grown and ligase induction, indicating that the increased in tissue culture, or cells infected with SV40, activity in infected cells probably represents de [r] ...
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Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast
... Even less is known about how the APC/C is inactivated as cells exit meiosis II. This is an important question as APC/C inactivation is important for normal embryonic development in Drosophila [40]. Similarly, we find ...
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Modulating Parkin E3 Ubiquitin Ligase Activity Using Phospho-Ubiquitin Variants
... Reactive oxygen species (ROS) are commonly observed at increased levels in brain tissue samples of individuals with AD, ALS and PD. Increased ROS is related to the relatively high metabolic and low cellular regeneration ...
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Human BRCA1 BARD1 Ubiquitin ligase activity counters chromatin barriers to DNA resection
... BRCA1 loss in murine cells has recently been associated with de-repression of heterochromatic silencing (Zhu et al., Nature, 2011. 477(7363): p. 179-84.). Similarly H2A mono-ubiquitination at K119 of H2A is associated ...
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Functional Characterization of Residues Required for the Herpes Simplex Virus 1 E3 Ubiquitin Ligase ICP0 To Interact with the Cellular E2 Ubiquitin-Conjugating Enzyme UBE2D1 (UbcH5a)
... This activity allows the virus to evade the cytotoxic T-cell ...ligase activity. While the RING finger domain of ICP0 has biochemical activity in vitro in the presence of UBE2D1 and UBE2E1 (2, ...
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Mind bomb 2, a founder myoblast specific protein, regulates myoblast fusion and muscle stability
... presumed ligase activity of the RING-finger domains appear dispensable for the Mib2 functions related to muscle stability, we inferred that Mib2 might have a structural role in maintaining muscle ...
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Ubiquitin phosphorylation in Parkinson’s disease: Implications for pathogenesis and treatment
... Mitochondrial dysfunction triggers PINK1 localization and activation on the OMM of damaged mitochondria [29, 35, 44, 49, 50], resulting in Ser65-phosphorylation of ubiquitin chains that are already conjugated to OMM pro- ...
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Massively Parallel Functional Analysis of BRCA1 RING Domain Variants
... E3 ligase activity for variants with missense substitutions ranged from completely nonfunctional (scores of zero) to nearly three times higher than wild ...E3 ligase scores to previous work by ...
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Kaposi's Sarcoma-Associated Herpesvirus RTA Promotes Degradation of the Hey1 Repressor Protein through the Ubiquitin Proteasome Pathway
... replication (18, 37). RTA controls lytic replication through transcriptional activation of a number of lytic genes including polyadenylated nuclear (PAN) RNA, K8, and viral interleu- kin-6 (18, 37, 45, 46, 49, 50, 66) ...
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N-Terminal Phosphorylation Sites of Herpes Simplex Virus 1 ICP0 Differentially Regulate Its Activities and Enhance Viral Replication
... ubiquitin ligase, ICP0 acts as a scaffold, binding both the E2 ubiquitin-conjugating (Ubc) en- zyme and its substrates, thereby facilitating ubiquitin chain transfer and conferring substrate ...Ub ligase ...
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