Lineage commitment
Single-cell reconstruction of differentiation trajectory reveals a critical role of ETS1 in human cardiac lineage commitment
... We next sought to determine the functional implications of such divergence and its possible role in cardiac lineage commitment. Analysis of differentially expressed genes (DEGs) between the cardiac ...
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The miRNA Biogenesis Machinery Modulates Lineage Commitment During αβ T Cell Development
... promote commitment to the CD8 and CD4 lineages, respectively. Thus, lineage commitment is impaired in the absence of ...and lineage commitment during αβ T cell ...
125
Geminin cooperates with Polycomb to restrain multi lineage commitment in the early embryo
... We previously defined Geminin (Gem or Gmnn) as another putative cell intrinsic regulator of early embryogenesis. Geminin is a small predominantly nuclear protein that can promote neural gene expression at the expense of ...
12
Side branching and luminal lineage commitment by ID2 in developing mammary glands
... There are four different forms of Inhibitor of DNA-binding (ID) protein: ID1, ID2, ID3 and ID4. Although they all have a helix-loop- helix (HLH) domain (Norton et al., 1998), their respective functions in mammary glands ...
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Global Analysis of Dynamic Epigenetic Marking and Transcriptional Regulation Underlying T-Cell Lineage Commitment
... settling progenitors, and PU.1 deficiency leads to a developmental block at DN1-like stage (Scott et al., 1994; Spain et al., 1999; Dakic et al., 2005). We have shown that PU.1 global occupancy pattern in DN1 (and DN2a) ...
182
Histone demethylase KDM2B regulates lineage commitment in normal and malignant hematopoiesis
... and Supplemental Table 2) preferentially enriched at gene promot- ers (Supplemental Figure 7B). KDM2B binds a core of 780 genes in all cell lines as well as distinct, cell-type–specific gene sets (Figure 9A). IPA of the ...
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Biophysical Regulation of Stem Cell Osteogenic Lineage Commitment: A Role for the Cytoskeleton
... The objective of this thesis was to investigate how changes in the bone marrow mechanical environment under physiologically relevant loading regimes can affect the osteogenic potential of mesenchymal stem cells; with a ...
196
Mechanically induced osteogenic lineage commitment of stem cells
... Upon application of oscillatory fluid flow, MSCs exhibited immediate increases in intracellular calcium mobilization. Twenty-four hours after fluid flow, proli- feration rates of human MSCs derived from bone mar- row ...
10
Hippo signaling promotes lung epithelial lineage commitment by curbing Fgf10 and β catenin signaling
... Growth factor signaling provides another layer of Yap/ β -catenin- mediated control over branching and differentiation programs, as Fgf10/Kras and Fgf10/ β -catenin signaling induce the expression of the branching ...
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Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points
... Results: To characterize the order of cell fate decisions during hematopoiesis, we collected primary cells from mouse bone marrow and isolated 10 hematopoietic populations to generate transcriptomes and genome-wide maps ...
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Developmental fate and lineage commitment of singled mouse blastomeres
... To visualize these data, we employed principal component analysis (PCA) based on the expression of these genes in the TE, ICM and 1/32LS blastomeres. Projection of the blastomere expression patterns onto component 1 and ...
10
T cell development and lineage commitment: Studies based on differential gene expression in thymocyte subsets
... Initial analysis of HY transgenic mice, in which the TCRaP transgene recognises a male-specific peptide in the context of H-2D^ (Fenton et al., 1988; Kisielow et al., 1988) seem ed to support the competitive model. ...
304
Numb regulates somatic cell lineage commitment during early gonadogenesis in mice
... Fig. 8. Culture with DAPT rescued some aspects of the Numb/Numbl mutant phenotype. E11.5 control and mutant gonads were dissected and cultured with DMSO or 100 µM DAPT for 48 h. (A,B) Control XY gonads treated with DMSO ...
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Fas Receptor Modulates Lineage Commitment and Stemness of Mouse Neural Stem Cells
... Figure 5. LPR NPCs have enhanced differentiation into Neuronal and OPC lineages. (a) Representative fluorescence histo- gram for A2B5 positivity after 2 days in minimal media (red = isotype control [ISO]); (b) ...
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Regulation of human hematopoietic stem cell lineage commitment
... My thesis projects focus on identifying and characterizing regulatory factors involved in myeloid commitment steps for human hematopoietic stem and progenitor cells (HSPCs), so as [r] ...
143
FOXF1 inhibits hematopoietic lineage commitment during early mesoderm specification
... regulators including NKX2.5, GATA4, HAND1/2 and MEF2C (Bondue et al., 2008; Lindsley et al., 2008). In contrast, little is known about the specification of the mesoderm to the mesothelium that forms an epithelial ...
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T-Lineage Specification and Commitment Requires Constraint of Myeloid Gene Expression Programs by Hes1
... of T-lineage commitment is thought to be complete by the DN2b stage, which is distinguished from the DN2a stage based on decreased levels of Kit expression. While DN2a and DN2b cells express CD44 and Kit, ...
177
The Effect of Cardiogenic Factors on Cardiac Mesenchymal Cell Anti-Fibrogenic Paracrine Signaling and Therapeutic Performance
... with lineage commitment-mediated adaptations in CMC anti-fibrogenic paracrine ...cardiomyogenic lineage commitment or simply off-target effects elicited by an otherwise non-specific epigenetic ...
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<p>Orcinol glucoside facilitates the shift of MSC fate to osteoblast and prevents adipogenesis via Wnt/β-catenin signaling pathway</p>
... Traditional Chinese medicine has been used to treat bone diseases and to promote bone healing, among which Rhizoma Curculiginis (Xianmao) is a well-known “ bone- tonifying ” herb. Rhizoma Curculiginis has been used to ...
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Mammary development in the embryo and adult: a journey of morphogenesis and commitment
... An interesting counterpoint to the STAT6 and GATA3 stories is the role of Notch signalling in lineage commitment. The Notch pathway is crucial also in Th1/Th2 determination (Ansel et al., 2006). Notch ...
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