Live cells
Protein fishing from single live cells
... of cells is lysed, the contents are separated by gel electro- phoresis, followed by detection using antibodies that tar- get the specific protein(s)-of-interest ...studying live cells ...of ...
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Interplay between distribution of live cells and growth dynamics of solid tumours
... MR1 cells (the original data are shown in panel C of figure 2 in ...of live cells F(r) from these experimental data by calculating the ratio between the measured rim thickness of viable cells ...
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Measuring DNA content in live cells by fluorescence microscopy
... staging live cells within the cell cycle but is limited temporally to the end of the time-lapse ...of live cell cycle reporters can be ...clarify cells that are in S phase from those in G1 and ...
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Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells
... in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical ...various live cancer cells ...
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Inferring diffusion in single live cells at the single-molecule level
... living cells is a fundamental feature of biological ...in live cells is fluorescence microscopy, especially so combining total internal reflection fluorescence (TIRF) with the use of fluorescent ...
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TOR1 and TOR2 Have Distinct Locations in Live Cells
... The concentration of TOR1-3XGFP near the vacuolar membrane is consistent with some reports in the literature. The loss of TOR1 is synthetically lethal with loss of class C VPS (vacuolar protein sorting) genes (43). This ...
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Improved molecular toolkit for cAMP studies in live cells
... cAMP is a major cellular second messenger that activates and integrates multiple intracellular signaling pathways and modulates a large range of cellular processes, includ- ing gene transcription [1], cell adhesion and ...
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Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
... However, one problem associated with incorporating live cells into an electrospun scaffold is the difficulty following aseptic technique. Due to undesirable paths to electrically ground the electrospinning ...
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Accurate position tracking of optically trapped live cells
... bone cells and used a viscous drag force method to calibrate the optical trapping force ...T cells and antigen presenting cells to orientate the cell pair with respect to the imaging plane of their ...
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Modelling the interactions between tumour cells and a blood vessel in a microenvironment within a vascular tumour
... of live cells and the oxygen tension attained maxima at the blood vessel (see Figures ...dead cells) due to oxygen starvation, is a transient property in all cases except where the oxygen consumption ...
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A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... with live cells expressing green fluorescent ...in live cells is exemplified by observation of time-dependent FRET between the Epithelial growth factor receptor (EGFR) and the adapter protein ...
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Monitoring Early Fusion Dynamics of Human Immunodeficiency Virus Type 1 at Single-Molecule Resolution
... Current assays cannot probe early fusion dynamics at single-molecule resolution in live cells and in real time. Traditional assays have provided an important mechanistic understanding of the fusion process, ...
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Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography
... on cells to provide complementary ...living cells. However, when comparing cells with different morphologies, it is difficult to know whether spectral differences arise from molecular differences ...
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Glutaraldehyde cross-linking of amniotic membranes affects their nanofibrous structures and limbal epithelial cell culture characteristics
... the Live/Dead Viability/Cytotoxicity Kit (Molecular Probes, Eugene, OR, USA), which contains calcein ace- toxymethyl and ethidium homodimer ...living cells, which cleaves the calcein acetoxymethyl to ...
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Clinical use of Dieletrophoresis separation for live Adipose derived stem cells
... Flow activated cytometry cell separation (FACS) or mag- netic antibody cell separation (MACS) have also been pro- posed as a solution, yet these too are fraught with difficulties of finding FDA approved good ...
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Factors influencing the reactivity of Legionella antigens in immunofluorescence tests
... On the basis of the above results, we used relative reactivity of live cells that were heat CYE agar at 25°C for experiments relative to the fixed on the slide, heat-killed cells, cells [r] ...
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Chemical Tools for Protein Imaging in Live Bacterial Cells
... cells did not express the target protein or were treated only with 1, did not show significant fluorescence (Figure S4.10 and S4.11). These results demonstrate that 4 and 5 should be broadly useful for resolving ...
185
Live imaging of multicolor labeled cells in Drosophila
... of live imaging would be that data could be collected at multiple time-points from the same ...from live imaging is analyses of animal behavior at single-cell ...tendon cells as they undergo complex ...
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Raman spectroscopy for grading of live osteosarcoma cells
... of cells but not tissues because Raman spectroscopy has the advantage of subcellular ...for live human tissues in a much shorter time frame with greater sensitivity and ...
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Efficiency of Cell-Free and Cell-Associated Virus in Mucosal Transmission of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus
... N ovel microbicide and vaccine candidates for human immu- nodeficiency virus type 1 (HIV-1) are being evaluated pre- clinically for efficacy by assessing their ability to protect nonhu- man primates against cell-free ...
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