loop-mediated isothermal DNA amplification
Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop Mediated Isothermal DNA Amplification with Hybridization to Species Specific Oligonucleotide Probes
... format. DNA amplification was achieved by the recently developed loop-mediated isothermal DNA amplification technology, a promising option for the development of improved ...
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Rapid Detection of Mycoplasma pneumoniae by Loop-Mediated Isothermal Amplification (LAMP) in Clinical Respiratory Specimens
... The LAMP assay is more sensitive and reliable than conventional culture and serology methods for detection of M. pneumoniae. In a cross- sectional study, the LAMP method was com- pared with culture and serology. The LAMP ...
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Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... DNA extraction. The DNA template for the nested PCR and LAMP was prepared as described previously (20). Twenty-five to 50 l of human blood was blotted as a single spot and dried on filter paper. A single ...
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Development and evaluation of an in house single step loop mediated isothermal amplification (SS LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients
... To verify their specificity for the detection of MTBC IS6110 sequence, our selected primers were tested using DNA from 60 MTBC positive isolates provided by the Pasteur Institute of Morocco. These MTBC isolates ...
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Rapid Diagnosis of Human Herpesvirus 6 Infection by a Novel DNA Amplification Method, Loop Mediated Isothermal Amplification
... viral DNA in PBMCs by sensitive PCR cannot differentiate between active infection and latent infection in organ trans- plant ...viral DNA in plasma correlates well with active viral infection ...plasmid ...
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A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification
... The Loop-mediated isothermal amplification (LAMP) is a biotechnique that can amplify deoxyribonucleic acid (DNA) within 60 minutes under isothermal conditions (50-65 ...the ...
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Loop mediated isothermal amplification for the detection of goose circovirus
... on loop-amplification of a specific site of the gene by Bst or Bsm polymerases with DNA-strand displacement ...of DNA strand but lack 5´ ! 3´ exonuclease ...on DNA strand displacement ...
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Loop Mediated Isothermal Amplification for Detection of African Trypanosomes
... Taq DNA polymerase is easily inactivated by tissue- and blood-derived inhibitors, such as myoglobin, heme-blood protein complex, and immunoglobulin G (1, 2, 5, ...
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Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
... Determination of sensitivity of LAMP assay Cloning of the considered locus in pTZ57R vector: TA cloning procedure was done using CloneJET™ PCR cloning kit (Thermo Scientific) according to the manufacturer's ...
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Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives
... non-specificity amplification from specificity amplification and false positive results are easy to appear; and fourthly, the LAMP products can’t be sequenced for the confirmation of the ...
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Loop-mediated isothermal amplification assays for screening of bacterial integrons
... the amplification principle, the specific LAMP reaction generated many ladder-like pattern bands on agarose gel due to its characteristic secondary structure, with sizes ranging from 193 bp for intI1, 148 bp for ...
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Feasibility and Operational Performance of Tuberculosis Detection by Loop Mediated Isothermal Amplification Platform in Decentralized Settings: Results from a Multicenter Study
... FIG 1 Schematic of the TB-LAMP procedure. A wide-bore disposable pipette is included in the kit to transfer 60 l of sputum to a heating tube containing extraction solution. A separate room or bench area is used for ...
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Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... recognize eight distinct regions in the target DNA. A large amount of product is formed, due to the autodisplacement activity of the enzyme, and because of this property, identifi- cation of a positive reaction ...
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Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... residual DNA, the RNA product was purified using a RNA purification kit (Tiangen, Beijing), and its concentration was determined spectrophotometrically using a NanoDrop 2000 (Thermo Table 2 Sources of pathogens ...
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Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification
... the DNA element and primer sets that specifically recognize this template, even in the presence of contaminating human or pathogen DNA, and (ii) a conjugation chemistry that bonds the DNA element to ...
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Development of a Loop Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples
... a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid inexpensive molecular diagnosis of this ...using DNA extracted from 91 clinical isolates of known geographic ...
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Mitochondrial DNA Targets Increase Sensitivity of Malaria Detection Using Loop Mediated Isothermal Amplification
... The mitochondrial primer sets used here recognized all mi- croscopy-positive clinical samples tested, and the high-density sequence data available for numerous malarial mitochondrial sequences isolated from parasites ...
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Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
... Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the rapid detection of the major periodontal pathogens Porphyromonas ...
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Detection of Human Herpesvirus 7 DNA by Loop Mediated Isothermal Amplification
... of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this ...HHV-7 DNA, neither HHV-6 nor human ...
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Evaluation of non instrumented nucleic acid amplification by loop mediated isothermal amplification (NINA LAMP) for the diagnosis of malaria in Northwest Ethiopia
... acid amplification loop-mediated isothermal amplification (NINA-LAMP) compared to standard thick and thin film microscopy and nested PCR as gold standard for the sensitive diagnosis of ...
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