mass spectrometric protein identification
Identification of Novel Diagnostic Serum Biomarkers for Chagas' Disease in Asymptomatic Subjects by Mass Spectrometric Profiling
... and 3). ApoA1 is the major structural and functional protein component of high-density lipoprotein (HDL), where it serves as a cofactor for esterification (15) of plasma cholesterol. In- teraction between T. cruzi ...
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Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences
... with identification of proteins through peptide mass fingerprinting (PMF) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is the widely used technique ...
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Constituents of SH1, a Novel Lipid-Containing Virus Infecting the Halophilic Euryarchaeon Haloarcula hispanica
... determined protein N termini, the initiating methionine had been re- ...seven protein bands yielding no detectable signals in N-terminal sequencing (VP5, VP6, VP8, VP11, VP13, VP14, and VP15) were all minor ...
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Label-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy
... cal identification of a large number of proteins with a changed ...brain-released protein biomarkers might be useful as predictive, diagnostic, prognostic and/or ther- apy-monitoring detection tools for the ...
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Automated integration of monolith based protein separation with on plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples
... analytes [47]. This is essential in proteomics studies, where a wide dynamic range of protein concentration presents a great challenge. Considering that the droplet size is dependent on the surface tension and ...
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Mass Spectrometric Identification of Proteotypic Peptides from Clinically Used Tumor Markers
... for protein identification in the mass spectrometry/proteomics facility at Johns Hopkins School of Medicine and the support of trans-proteomic pipeline Software tools available from Aebersold group ...
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MALDI TOF Mass Spectrometric Analysis of Brain Tumor Cyst Fluid Reveals a Protein Peak Corresponding to ApoC1 and LuzP6
... DOI: 10.4236/ojmn.2018.83021 261 Open Journal of Modern Neurosurgery On the other hand, high molecular weights are difficult to analyze, since our analysis algorithm allows for a deviation of 0.3% according to the ...
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A proteome-wide immuno-mass spectrometric identification of serum autoantibodies
... CUZD1 protein (see below) for 2 h with ...CUZD1 protein in 6% bovine serum albumin (BSA) or with pancreatic lysate (extracted from human pancreas) diluted 100-fold in 6% ...
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Mass Spectrometric Identification of Mtb81, a Novel Serological Marker for Tuberculosis
... Knowledge of serological markers for TB patients coin- fected with HIV would facilitate the development of diagnostic tests for TB. A study designed to characterize the target of the antibody response revealed that an ...
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Analysis of dynamic tyrosine phosphoproteome in LFA 1 triggered migrating T cells
... The identification of proteins undergoing tyrosine phosphorylation in response to LFA-1 integrin stimulation is essential for understanding the molecular mechanisms and relevant signalling pathways that regulate ...
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In-depth mass spectrometric mapping of the human vitreous proteome
... with their accession number, molecular weight, isoelectric point (pI), Mascot score, number of unique peptides and sequence coverage. 242 of these proteins were found in all of the three samples, 137 in two samples, ...
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Mass Spectrometric Analysis of Organic Aerosol Composition: Laboratory and Ambient
... Organic matter is ubiquitous in atmospheric aerosols and ac- counts for a major fraction of particulate matter mass (Zhang et al., 2007a). Most particulate organic matter (POM) is sec- ondary in origin, comprising ...
203
Centrosomes in the zebrafish (Danio rerio): a review including the related basal body
... centrosomal protein, called “ pericentrin, ” with a molecular weight of about 220 kDa ...binding protein called CDK5RAP2 via a CM-2-like motif that is also conserved in zebrafish ...
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Modern mass spectrometric techniques applied to occupational hygiene analysis
... IPBC has been used as a consumer and industrial fungicide in the production o f paints, vinyl wallpapers, wall paper adhesives, metal working fluids, and cosmetics [1]. The author is aware o f one other group which has ...
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Mass spectrometric studies of ionization in shock heated gas mixtures
... 2.. Arrangement of accelerating slits. x-t diagram near end wall. Arrhenius plot of argon ions. Arrhenius plot of hydrogen ions.. The predominant impurity ions can be found a[r] ...
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Determination of Isotopic Composition and Concentration of Uranium and Boron by Thermal Ionization Mass Spectrometric Isotopic Dilution Technique
... Precision and accuracy of boron measurements were calculated and compared with the certified value as shown in Table 1. To evaluate the net peak heights of the masses 88 and 89, a correction was made for oxygen-17 ...
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Analysis of intravenous lipid emulsions and their effect on cellular structure and function
... lonisation-Tandem Mass Spectrometry As shown in Figures 5-3 PE species were not detected in positive ion ...that identification of PE ions can be achieved with negative ion ESI- MS/MS by employing a ...
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The study of thione thiol tautomerism of 4 amino 5 (4 nitrophenyl) 2,4 dihydro 3H 1,2,4 triazole 3 thione by HPLC MS method
... Interpretation of the mass spectrum b Fig.3 based on chromatographic and mass spectrometric data Peak, releasing on the 8,552 minute can be characterized on the basis of analysis of the [r] ...
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Fundamental studies and instrumental methodology in the mass spectrometric analysis of low mass polymeric systems
... alone sufficient achieve an for large ionisation aggregate systems still generally unknown although potentials with they would be theorised as being lower that the corresponding individu[r] ...
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Application of tandem two dimensional mass spectrometry for top down deep sequencing of calmodulin
... Investigation of the horizontal scans represents the equivalent of adding MS 3 information to the MS 2 information provided by the analysis of the autocorrelation line. The cleavage coverage map of Figure 3.a shows in ...
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