mature DCs
Melanoma immunotherapy using mature DCs expressing the constitutive proteasome
... antigen-loaded DCs can stimulate immune tolerance, almost all clinical DC-based immunotherapy trials have used DCs that have been induced to ...tion, DCs express increased levels of costimulatory ...
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Spatial organization of dendritic cells within tumor draining lymph nodes impacts clinical outcome in breast cancer patients
... of DCs with T ...clustered DCs did not necessarily have a higher number of mature DCs and vice ...clustered DCs was significantly greater than the number of T cells around unclustered ...
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<p>IL-37 induces anti-tumor immunity by indirectly promoting dendritic cell recruitment and activation in hepatocellular carcinoma</p>
... Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (Guangzhou Blood Center, Guangzhou, China) by Ficoll-Hypaque density gradient centrifugation. PBMCs were then seeded (1×10 8 cells) into 75 ...
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Dendritic Cells from Chronic Lymphocytic Leukemia Patients Are Normal Regardless of Ig V Gene Mutation Status
... in mature DCs were not significantly different between the 2 B-CLL sub- ...to DCs generated from normal age-matched ...and mature DCs was small to negligible for both B-CLL-DCs ...
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Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow derived dendritic cells mediated toll like receptor 4
... Im- mature DCs have high antigen-uptake abilities, but after maturation, the cells lose this ability and mature into APCs ...whether mature DCs exhibited reduced antigen-uptake ...of ...
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Original Article Therapeutic and anti-metastatic effect of CIKs on cervical cancer enhanced by modified HPV16 E7-pulsed DCs with SOCS1-knockdown
... The DCs were then pulsed with the HPVm16E7 pro- tein at 25 µg/mL (HPVm16E7 was prepared as we previously reported in [22]) for 6 h, followed by stimulation with TNF-α (10 ng/mL) for 24 h to develop mature ...
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Critical role of the Toll like receptor signal adaptor protein MyD88 in acute allograft rejection
... The Toll-like receptors (TLRs) are recently discovered germline-encoded receptors on APCs that are critically important in innate immune recognition of microbial pathogens. However, their role in solid-organ ...
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Original Article Distribution and expression profiles of dendritic cell subpopulations in human bladder cancer
... Abstract: Bladder cancer is one of the most common cancers worldwide, with transitional cell carcinoma (TCC) being the predominant form. Dendritic cells (DCs) are a heterogeneous population of antigen-presenting ...
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Infectious and Whole Inactivated Simian Immunodeficiency Viruses Interact Similarly with Primate Dendritic Cells (DCs): Differential Intracellular Fate of Virions in Mature and Immature DCs
... immature DCs are susceptible to low-level productive infection with R5 HIV-1 strains, viral replication cannot be achieved in mature ...and mature DCs are able to trap and then transmit both ...
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Original Article Suppressive effect of β, β-dimethylacryloyl alkannin on activated dendritic cells in an imiquimod-induced psoriasis mouse model
... by mature DCs, and hence, it is inferred that the alkannin has shown some suppressive effect on ...in mature DCs derived from human peripheral blood monocytes, suppress the expression of IL-6, ...
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Type I Interferon Limits the Capacity of Bluetongue Virus To Infect Hematopoietic Precursors and Dendritic Cells In Vitro and In Vivo
... BM-derived DCs in the presence of GM-CSF (see Materials and Methods), restricting our study to myeloid precursors, we have approached this issue by infecting BM cells (HSCs) and BM-derived DCs at different ...
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Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine induced killer cells and dendritic cells in vitro
... and DCs were generated from peripheral blood mononuclear cells (PBMCs) of healthy donors and char- acterized as described previously ...into mature DCs (mDCs) (Additional file ...
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Three-Dimensional Imaging of HIV-1 Virological Synapses Reveals Membrane Architectures Involved in Virus Transmission
... by mature DCs (16), and inhibiting the function of the Rho family GTPase Cdc42 dramat- ically reduces the number of membrane protrusions and decreases HIV-1 transfer at infectious synapses formed with ...
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Mature dendritic cells boost functionally superior CD8+ T cell in humans without foreign helper epitopes
... of DCs, with enhanced capacity to stimu- late T-cell immunity ...monocyte-derived, mature DCs leads to rapid enhancement of T- cell responses in humans ...that mature DCs are potent ...
7
Effect of Oxygen Levels on the Physiology of Dendritic Cells: Implications for Adoptive Cell Therapy
... using DCs and T cells from different ...and mature DCs were generated and activated as above from HLA-A*0201–positive donors, pulsed with 100 ng/mL HLA- A*0201– specific MART1 peptide and cocultured ...
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Glycosphingolipid Composition of Human Immunodeficiency Virus Type 1 (HIV-1) Particles Is a Crucial Determinant for Dendritic Cell-Mediated HIV-1 trans-Infection
... and mature DCs can mediate transfer of captured HIV-1 par- ticles to CD4 ⫹ T cells, a mechanism of HIV-1 trans-infection, and DC–T-cell cocultures have been shown to support explo- sive virus replication ...
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A potential role of karyopherin a2 in the impaired maturation of dendritic cells observed in glioblastoma patients
... total mature DCs as well as subsets of mature DCs in patients with glioblastomas were as following: total DCs as proportion of WBC: ...total DCs: ...total DCs: ...of ...
7
Defining novel parameters for the optimal priming and expansion of minor histocompatibility antigen specific T cells in culture
... Antigen-specific T cell lines were generated using 15 × 106 PBMCs as responder cells and cocultured with autologous, peptide-loaded mature DCs as antigen presenting cells APCs at a 1:10 [r] ...
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Original Article MiR-15a promotes phenotypic and functional maturation of LPS-induced dendritic cells through down-regulating DDX39 expression
... significance. DCs are in the central part of the adaptive immunity and play a key role in chronic rejection [12, ...antigens, DCs activate the T cell response by uptaking, processing and presenting the ...
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Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivoin HLA DR1 transgenic mice
... The importance of our finding that blockade of CII uptake pre- vents activation of specific T cells in vitro was tested in vivo. We administered amiloride in vivo and showed reduction in the magnitude of CII-specific, ...
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