mecA gene
Comparison of Xpert MRSA/SA Nasal and MRSA/SA ELITe MGB Assays for Detection of the mecA Gene with Susceptibility Testing Methods for Determination of Methicillin Resistance in Staphylococcus aureus Isolates
... the mecA gene, en- coding a specific methicillin-resistant transpeptidase (penicillin- binding protein 2a [PBP2a]) (1), which confers resistance to all  -lactam antibiotics due to low-affinity binding, ...
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Loss of the mecA Gene during Storage of Methicillin Resistant Staphylococcus aureus Strains
... (25.7%) mecA gene-pos- itive strains from the 35 Microbank ...the mecA was detected by PCR directly on a ...of mecA gene-positive isolates; a total of seven mecA ...
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Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of the mecA Gene
... The Portrait Staph ID/R blood culture panel (BCP) is a qualitative, multiplex, nucleic acid-based assay that simultaneously identifies Staphylococcus aureus, Staphylococcus lugdunensis, and various Staphylococcus species ...
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Antimicrobial resistance phenotype and analyses of meca gene in staphylococcus aureus from cheese in Brazil
... The sensible PBP2 protein is normally present in bacteria, participating in the synthesis of cell wall peptidoglycan. In the presence of penicillin, PBP2 protein is inhibited by the binding of the antibiotic to its ...
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Determination of the diversity and antibiotic resistance profiles of Staphylococcus species from dogs with otitis externa and examination of mecA gene occurrence
... Studies on methicillin-resistant staphylococci are focused mainly on farm animals, in Turkey. There have also been a few studies in dogs (Findik et al. 2009; Bagcigil et al. 2012). Findik et al. (2009) re- ported the ...
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Antimicrobial resistance profile and molecular detection of MecA gene in methicillin resistant Staphylococcus aureus from patients in selected general hospitals in Abuja municipal, Nigeria
... were mecA gene positive and is similar to studies previously reported [50-51] and this shows that resistance to oxacillin may not necessarily be due to modification of penicillin binding protein which is ...
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Staph ID/R: a Rapid Method for Determining Staphylococcus Species Identity and Detecting the mecA Gene Directly from Positive Blood Culture
... confused with Staphylococcus species in Gram stain analysis. Be- cause E. faecalis is often confused with Staphylococcus species in Gram stain analysis and is specifically detected with this assay, we added it to the ...
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Use of a Three Dimensional Microarray System for Detection of Levofloxacin Resistance and the mecA Gene in Staphylococcus aureus
... Evaluation of clinical isolates. Microarray analysis was per- formed under both 0.5 ⫻ SSPE and 6 ⫻ SSPE postwashing conditions in 24 MRSA isolates and 3 S. aureus isolates in duplicate. In the RDBH analysis for the ...
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mecA Gene Is Widely Disseminated in Staphylococcus aureus Population
... the mecA gene, which encodes the low-affinity penicillin-bind- ing protein PBP 2A ...The mecA gene is part of a 21- to 60-kb staphylococcal chromosome cassette mec (SCCmec), a mobile genetic ...
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Frequency of MecA Gene in the Clinical Isolates of Staphylococcus epidermidis in Isfahan, Iran
... the mecA gene (F: TGCTATCCACCCTCAA ACAGG; R: AACGTTGTAACCACC CCAAGA) as per the following instructions: 1 µl of the extracted DNA and 1 µl of each primer was added to 12 µl the PCR Master kit (Kappa, USA), ...
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Rapid detection of the mecA gene in methicillin resistant staphylococci by enzymatic detection of polymerase chain reaction products
... Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans[r] ...
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Methicillin Resistant Staphylococcus aureus: Comparison of Susceptibility Testing Methods and Analysis of mecA Positive Susceptible Strains
... the mecA gene used as the “gold standard” ...lacked mecA was weakly positive by the MRSA-Screen latex agglutination ...the mecA gene by Southern blot ...that mecA- and PBP ...
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Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic Device Related Infections Correlated with Patient Outcome
... the mecA gene), supporting previously reported trends ...mec gene complex, including the methicillin resistance gene ...the mecA gene, indicative for the presence of the mobile ...
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Molecular Characterization of Staphylococcus sciuriStrains Isolated from Humans
... sciuri mecA) closely related to the mecA gene of methicillin-resistant Staphylococcus aureus (MRSA) ...the gene, identical to MRSA ...sciuri mecA copy, sustaining our previous proposal ...
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Hypervariability of Biofilm Formation and Oxacillin Resistance in a Staphylococcus epidermidis Strain Causing Persistent Severe Infection in an Immunocompromised Patient
... and mecA-positive wild-type genotype, with the selection and predom- inance of strongly biofilm-forming and oxacillin-resistant variants in the later stages of the ...
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Evaluation of the BD Phoenix Automated Microbiology System for Identification and Antimicrobial Susceptibility Testing of Staphylococci and Enterococci
... included. MecA gene testing with the Light- Cycler instrument (Roche Molecular Systems, Pleasanton, CA) was used to confirm any discrepant results for oxacillin obtained by the agar method and with the ...
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Isolation of methicillin resistant coagulase negative staphylococci from chickens
... With respect to animal MRSA isolates Devriese and Hom- mez (5) reported the isolation of MRSA from the milk of mastitic cows in 1975. From the results of phage typing and as a result of some of the biological ...
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Use of a DNA Microarray for Simultaneous Detection of Antibiotic Resistance Genes among Staphylococcal Clinical Isolates
... resistance gene-positive ...but mecA-positive CoNS (n ⫽ 17), pre- sumably because of the known heterogeneous expression of mecA gene in Staphylococcus in vitro ...resistance gene that ...
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Molecular Identification of Methicillin Resistant Staphylococcus (Mrsa) by Polymerase Chain Reaction (Pcr) Technology
... Staphylococcus aureus to greater than 5 x 10(3) CFU per tube for Staphylococcus epidermidis. Genotyping results obtained by ED-PCR of 161 tested strains from the colonies (97 strains of S. aureus and 64 strains of ...
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Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA
... the mecA probe and PCR results might reflect ...the mecA gene, three different mutations yielding a false- negative result are ...the mecA probe and PCR was different from the colonies used ...
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