MIN6 cells
Transcriptome Profiles and Gene Expression of Min6 Cells Are Altered by Pancreatic Stellate Cells
... from Min6 cells employing RNeasy kit (Qiagen, Ger- many) as per the manufacturer’s ...cocultured Min6 cells samples employing Ion Total RNA-Seq kit V2, including Ion RNA-Seq Core kit v2, Ion ...
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Lipotoxicity is glucose-dependent in INS-1E cells but not in human islets and MIN6 cells
... INS-1E cells but not in islets obtained from healthy individuals and in MIN6 cells might be explained by limited inhibition of fatty acid oxidation by malonyl-CoA in the latter and may speak in favor ...
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Original Article Effect of glucagon on insulin secretion through cAMP signaling pathway in MIN6 cells
... of MIN6 cells were trypsin- ized, 1000 rpm centrifuged at room tempera- ture for 5 minutes, then added 100 µl electro- poration buffer and 10 µg ICUE3 or ...transfection, cells were cultured in ...
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Suppression of transcription factor PDX 1/IPF1/STF 1/IDX 1 causes no decrease in insulin mRNA in MIN6 cells
... Suppression of PDX-1 expression by antisense ODN. Using the antisense ODN strategy, we suppressed the expression of PDX-1 in the pancreatic b cell–derived MIN6 cells (19). A set of antisense, sense, and ...
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Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-κB/c-Myc Pathway in Pancreatic β cells
... treatment, MIN6 cells were collected for immunoprecipitation of RIP1 using an antibody against ...of MIN6 was remarkably disturbed by ...and MIN6 cell ...
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Loss of Nix in Pdx1 deficient mice prevents apoptotic and necrotic β cell death and diabetes
... insulinoma MIN6 cells to deplete Pdx1 and found that expression of proapoptotic genes, including NIP3-like protein X (Nix), was ...in MIN6 and pancreatic islet b cells induced programmed cell ...
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Reduced levels of SCD1 accentuate palmitate-induced stress in insulin-producing β-cells
... We next used a proteomic approach to identify mechan- isms by which reduced SCD1 levels affected MIN6 cells. Cellular proteins obtained from SCD1 KD and mock- transfected cells were separated, ...
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Native low density lipoprotein induces pancreatic β cell apoptosis through generating excess reactive oxygen species
... of MIN6 cells were assessed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine tri- phosphate-biotin nick end labeling (TUNEL) method using an in situ apoptosis kit (Takara, Otsu, ...the ...
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Detecting de novo insulin synthesis in embryonic stem cell derived populations
... labeled MIN6 lysate and plotting this against the concentration of insulin as measured by ELISA in this dilution series ...in MIN6 cells routinely down to levels below 5 ng/ml, we were unable to ...
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In vitro Enhancement of Pancreatic Cells MIN6 Proliferation by Insulinotropic Gymnema sylvestre Aqueous Extracts: Evidence-based Regenerative Therapeutic Capacity of a Medicinal Herb
... kit. MIN6 cells were subcultured on 96-well plates (10,000 cells/well) in growth medium containing different concentrations of ...of MIN6 cells was evaluated with a colorimetric ELISA- ...
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TDP 43 regulates early phase insulin secretion via CaV1 2 mediated exocytosis in islets
... in MIN6 cells by performing in vitro knockdown ...in MIN6 cells (Figure ...into MIN6 cells with mCherry (Figure ...mCherry-positive MIN6 cells with glucose ...
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Coenzyme Q10 suppresses apoptosis of mouse pancreatic β-cell line MIN6
... line MIN6 and staurosporine (STS). Here, we report that CoQ10 protects MIN6 cells against apoptosis caused by STS and describe the more detailed apoptotic ...of MIN6 cells was induced ...
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Myosin Va transports dense core secretory vesicles in pancreatic MIN6 β cells
... PC12 cells (Rudolf et ...(A) MIN6 cells were transfected with 60 pmol of anti-MyoVa or scrambled siRNAs (see Materials and ...(B) Cells were transfected with siRNA for 36 h then infected with ...
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Conditional ablation of HDAC3 in islet beta cells results in glucose intolerance and enhanced susceptibility to STZ-induced diabetes
... line, MIN6 cells with siRNA-mediated HDAC3 silence, showed decreased insulin gene transcription, which was mediated, at least partially, through the upregulation of suppressors of cytokine signaling 3 ...
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Metformin Plays a Dual Role in MIN6 Pancreatic β Cell Function through AMPK-dependent Autophagy
... medium; MIN6 cells, mouse insulinoma cells; 3-MA, 3-methyladenine; AICAR, 5-Aminoimidazole- 4-carboxyamide ribonucleoside; DMSO, dimethyl sulfoxide; PS, phosphotidylserine; mTOR, mamma- lian target ...
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Pancreas enriched miRNA refines endocrine cell differentiation
... Fig. 5. Overexpression of miR-7 in vivo reduces the expression of endocrine genes. (A) Schematic of the targeting construct for conditional miR-7-IRES-GFP misexpression. LoxP sites are indicated by arrowheads. (B) miR-7 ...
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Original Article miR-375 is downregulated by promoter hypermethylation in MIN6 insulinoma cells
... insulinoma MIN6 cells by Mas- sARRAY spectrometry and employed the DNA demethylating drug 5-aza-2’-deoxycytidine (5-aza-CdR) to treat MIN6 cells to explore the methylation patterns of the ...
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The protective effect of peroxiredoxin II on oxidative stress induced apoptosis in pancreatic β-cells
... of MIN6 β -cell line with proinflammatory cytokines, palmitic acid or streptozotocin dose- or time-dependently increased apoptosis, which was associated with reduced endogenous expression levels of ...in ...
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Atherogenic, fibrotic and glucose utilising actions of glucokinase activators on vascular endothelium and smooth muscle
... beta cells leading to increased glucose uptake and insulin production and secretion ...rat MIN6 pan- creatic beta cells expressing GK enzyme and found that GKAs (RO28-1675) and Compound A both ...
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Wolfram syndrome 1 gene negatively regulates ER stress signaling in rodent and human cells
... 832/13 cells overexpressing ...these cells, whereas there was no significant change in protein levels of the other 2 master regu- lators of the UPR, IRE1 and PERK (Figure ...β cells overexpressing ...
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