minus-strand RNA synthesis
The AAUAAA Motif of Bamboo Mosaic Virus RNA Is Involved in Minus-Strand RNA Synthesis and Plus-Strand RNA Polyadenylation
... positive-sense RNA genome with a 5 ⴕ -cap structure and a 3 ⴕ poly(A) ...genomic RNA appeared to diminish coat protein accumulation to 2% ...with RNA transcript, the accumulations of viral coat ...
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Recognition of the Core RNA Promoter for Minus-Strand RNA Synthesis by the Replicases of Brome Mosaic Virus andCucumber Mosaic Virus
... in minus-strand RNA synthesis by the BMV and CMV replicases, our results suggest that different features presented by the SLC are recognized by the two ...restored RNA synthe- sis to ...
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Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers.
... BMV RNA 3 were changed from the wild-type sequence of 5 9 -ACCA-3 9 to 5 9 -GCCA-3 9 ...stimulated minus-strand RNA synthesis to the same level that GpG did, would no longer be ...
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Alphavirus Minus-Strand RNA Synthesis: Identification of a Role for Arg183 of the nsP4 Polymerase
... viral RNA-dependent RNA polymerase activities ...genome RNA and copies it into a minus-strand template. The minus strand, in turn, is used as a template to produce both ...
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Specific Sindbis virus-coded function for minus-strand RNA synthesis.
... In cultures infected with tsll of complementation group B, the synthesis of viral minusstrand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h[r] ...
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Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis.
... 1874-1885 0022-538X/94/$04.00+0 Copyright © 1994, American Society for Microbiology Regulation of Sindbis Virus RNA Replication: Uncleaved P123 and nsP4 Function in Minus-Strand RNA Synt[r] ...
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Identification of the cis-acting signal for minus-strand RNA synthesis of a murine coronavirus: implications for the role of minus-strand RNA in RNA replication and transcription.
... We further showed that the DI clones which actively transcribed a subgenomic mRNA from an internal intergenic sequence synthesized much less minus-strand RNA than those clones which did [r] ...
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Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis.
... The replication of genomesized plus- and minus-strand RNA by the rhabdovirus vesicular stomatitis virus required continued synthesis of viral nucleocapsid protein 3, 4, 16, 32, but the t[r] ...
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In Vitro Synthesis of Minus-Strand RNA by an Isolated Cereal Yellow Dwarf Virus RNA-Dependent RNA Polymerase Requires VPg and a Stem-Loop Structure at the 3′ End of the Virus RNA
... plus-strand RNA genomes lack a 7-meth- ylguanosine cap structure but instead have a covalently linked virus-encoded protein (VPg) at the 5 ⬘ ...plus-strand RNA viruses, which are ...
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Requirements at the 3′ End of the Sindbis Virus Genome for Efficient Synthesis of Minus-Strand RNA
... for minus-strand ...genomic RNA has previously been proposed as the initiation site for minus-strand RNA synthesis, giving rise to minus-strand RNA ...
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The Synthesis of Minus-Strand RNA of Bamboo Mosaic Potexvirus Initiates from Multiple Sites within the Poly(A) Tail
... an RNA pseudoknot required for BaMV RNA replication. Recombinant RNA-dependent RNA polymerase (RdRp) of BaMV binds to the pseudoknot poly(A) tail in gel mobility shift assays ...initiate ...
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De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase
... viral RNA synthesis by transfection of BHK-21 cells with full-length RNA transcribed in vitro from wild-type and mutant EAV cDNA ...protein synthesis was monitored by immunofluorescence assays ...
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Sindbis virus RNA-negative mutants that fail to convert from minus-strand to plus-strand synthesis: role of the nsP2 protein.
... plus-strand RNA viruses, including members of the alphavirus superfamily (1, 9, 11, ...expressed RNA-stimulated ATPase and GTPase activities but not unwinding activity ...plus- strand ...
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A Pseudoknot in a Preactive Form of a Viral RNA Is Part of a Structural Switch Activating Minus-Strand Synthesis
... ization of positions 312 to 327 and selection for satC fitness in six plants (in vivo functional selection) revealed that all satC recovered in the initially inoculated plants contained sequence between positions 312 to ...
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Rotavirus RNA Replication Requires a Single-Stranded 3′ End for Efficient Minus-Strand Synthesis
... the RNA polymer- ...G8-59-B1 RNA can efficiently bind VP1 but is replication defective supports the conclusion that the association of the polymerase with the template RNA is not the sole factor ...
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Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis.
... These data showed that increased replication of the CR3.36 and CR3.39 genomes occurred during complementation and indicate that the ts defect is most likely a defect in nsP3 or nsP3-cont[r] ...
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Specific Interaction of Heterogeneous Nuclear Ribonucleoprotein Particle U with the Leader RNA Sequence of Vesicular Stomatitis Virus
... 39-terminal RNA sequence of the genome RNA is the binding site of the VSV RNA polymerase (2, 14, 15) to initiate ...genome RNA and its complement (leader-sense [LS]) RNA are the two ...
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The role of the pseudoknot at the 3' end of turnip yellow mosaic virus RNA in minus-strand synthesis by the viral RNA-dependent RNA polymerase.
... endogenous RNA, which is suggested to be of negative polarity ...both minus- and plus-strand production, the protein com- position of the RdRp we use to transcribe positive-sense RNA may ...
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Characterization of a Novel 5′ Subgenomic RNA3a Derived from RNA3 of Brome Mosaic Bromovirus
... positive-sense RNA viruses utilize various strategies for expression of their RNA genomes (36), most notably via subgenomic RNAs ...viral RNA polymerase (RdRp) on genomic minus-sense RNAs (20, ...
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Synthesis of plus- and minus-strand RNA in rotavirus-infected cells.
... This excess may be possible if preexisting plus-strand RNA Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have made a comprehensive study of the synthesis of viral [r] ...
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