Mouse hepatitis virus A59
Effect of Ligands to Toll Like Receptors (TLR) 3, 7 and 9 on Mice Infected with Mouse Hepatitis Virus A59
... with mouse hepatitis virus A59 (MHV-A59), an enveloped, positive-strand RNA Co- ronavirus, induce hepatitis, thymus involution, IgG2a-restricted hypergammaglobulinaemia, ...
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Thymus involution induced by mouse hepatitis virus A59 in BALB/c mice.
... Mouse hepatitis virus A59 (MHV-A59) infection of adult BALB/c mice induced a severe, transient atrophy of the thymus. The effect was maximal at 1 week after infection, and thymuses ...
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Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3.
... 3 Mouse Hepatitis Virus A59: mRNA Structure and Genetic Localization of the Sequence Divergence from Hepatotropic Strain MHV-3 Received 9 April 1981/Accepted 26 May 1981 The composition [r] ...
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Restricted replication of mouse hepatitis virus A59 in primary mouse brain astrocytes correlates with reduced pathogenicity.
... Virus-specific proteins present in Sac- cells and mouse brain astrocytes infected with wild-type wt MHV-A59 or its mutant ts342.. Cells were labeled with [35S]methionine.[r] ...
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Targeted Recombination within the Spike Gene of Murine Coronavirus Mouse Hepatitis Virus-A59: Q159 Is a Determinant of Hepatotropism
... recombinant virus wtR10 demyelinated to a similar extent as wild-type parental virus; this level of demyelination is similar to that we have previously reported for wild-type virus ...
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RNA recombination of murine coronaviruses: recombination between fusion-positive mouse hepatitis virus A59 and fusion-negative mouse hepatitis virus 2.
... In addition, both ML-11 and ML-3 probably contain multiple crossovers in gene A, although the presence of the crossovers in gene A of ML-11 is less certain, since only one detectable oli[r] ...
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Binding of the coronavirus mouse hepatitis virus A59 to its receptor expressed from a recombinant vaccinia virus depends on posttranslational processing of the receptor glycoprotein.
... Expression from the vaccinia virus recombinant Vac-MHVR in BHK-21 cells resulted in high levels of MHVR glycoprotein on the cell surface and made these cells susceptible to MHV-A59 infec[r] ...
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Identification of H209 as Essential for pH 8-Triggered Receptor-Independent Syncytium Formation by S Protein of Mouse Hepatitis Virus A59
... pH-triggered and receptor-independent cell-cell fusion assays. Human 293T cells were cotrans- fected with plasmids encoding MHV-A59 S glycoprotein and GFP using PEI. Twenty-four hours later, cells were fed with ...
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Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59.
... We have now characterized the murine CGMs expressed in BALB/c, SJL/J, and CD-1 liver and intestine cells, developed probes specific for mRNAs encoding several different murine CGMs, anal[r] ...
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Fusion-defective mutants of mouse hepatitis virus A59 contain a mutation in the spike protein cleavage signal.
... Mutant viruses isolated from infected glial cultures 16 to 18 weeks postinfection replicate with kinetics similar to those of wild-type virus but produce small plaques on fibroblasts and[r] ...
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Mouse Hepatitis Coronavirus A59 Nucleocapsid Protein Is a Type I Interferon Antagonist
... I virus exhibited resistance to IFN treatment comparable to that of the parental WT infectious clone virus ...the virus, consistent with what was observed for the WT MHV and ⌬ E3L N recombinant ...
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Intracellular Localization and Protein Interactions of the Gene 1 Protein p28 during Mouse Hepatitis Virus Replication
... chitis virus p15 homolog (p16) dimerizes via multiple cysteine residues ...bronchitis virus p16, including complete con- servation of cysteine residues, suggesting that these proteins have similar functions ...
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The function of the spike protein of mouse hepatitis virus strain A59 can be studied on virus-like particles: cleavage is not required for infectivity.
... Previous studies on the relation between cleavage of S and fusion have produced conflicting results. Rapid fusion of L cells induced by MHV-A59 virions purified from 17Cl1 cells required the addition of trypsin ...
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Mutational Analysis of the Virus and Monoclonal Antibody Binding Sites in MHVR, the Cellular Receptor of the Murine Coronavirus Mouse Hepatitis Virus Strain A59
... for virus receptor activity and MAb CC1 receptor ...MHV- A59 at a multiplicity of infection of 1. At 16 h after virus inoculation, the cells were fixed with cold ...convalescent mouse anti-MHV ...
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Importance of the Penultimate Positive Charge in Mouse Hepatitis Coronavirus A59 Membrane Protein
... the mouse hepatitis coronavirus (MHV) A59 M protein was suggested to participate in intermolecular interactions with negative charges in the nucleocapsid (N) ...
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Characterization of two temperature-sensitive mutants of coronavirus mouse hepatitis virus strain A59 with maturation defects in the spike protein.
... The S protein of wt MHV-A59 is synthesized and cotrans- lationally N-glycosylated in the ER to a 150K protein. Folding and oligomerization take place with a half-time of approxi- mately 1 h (21, 37), a process ...
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The murine coronavirus mouse hepatitis virus strain A59 from persistently infected murine cells exhibits an extended host range.
... coronavirus mouse hepatitis virus strain A59 (MHV-A59), expression of the virus receptor glycoprotein MHVR was markedly reduced ...1995). Virus isolated from passage 600 ...
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Genetic Determinants of Virulence for the A59 and JHM Strains of Mouse Hepatitis Virus
... for A59 to disrupt sequences that were toxic and/or unstable in bacteria ...Since A59 and JHM are 94% identical, the genome was divided into fragments in the same fashion, assuming the toxic regions would ...
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Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59
... to assemble a full-length infectious cDNA of TGEV except that it was not usually necessary to introduce mutations and new restriction sites into the wild-type virus genome to direct the assembly cascade (52). ...
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Characterization of the Coronavirus Mouse Hepatitis Virus Strain A59 Small Membrane Protein E
... While numerous viruses have been shown to variously in- duce novel structures in infected cells, rarely have these struc- tures been assigned to the action of a particular viral protein. An interesting exception are the ...
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