Multiphoton excitation
Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation
... pulsed-laser excitation beam which may then be scanned across a sample to construct a fluorescence ...that multiphoton excitation shifts the required wavelength to the red or near-infrared (NIR) ...
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Three dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation
... pulsed-laser excitation beam which may then be scanned across a sample to construct a fluorescence ...that multiphoton excitation shifts the required wavelength to the red or near-infrared (NIR) ...
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Long-lived metal complexes open up microsecond lifetime imaging microscopy under multiphoton excitation: from FLIM to PLIM and beyond
... of multiphoton absorption is critically depen- dent on the laser peak power, and so the energy per laser pulse was increased whilst reducing the repetition rate of the laser together with the average power at the ...
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Investigation of multiphoton induced fluorescence from solutions of 5 hydroxytryptophan
... by multiphoton excitation of the normal UV emission, but not by hyperluminescence, has also been reported 22,23 ...such multiphoton excitation indicate that the fluorescence lifetime is ...
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Nanoscale hydroxyl radical generation from multiphoton ionization of tryptophan
... nm is not observed). Addition of millimolar concentrations of hydrogen peroxide resulted in increased emission intensity at 500 nm characteristic of luminescence from the 5HTrp photoproduct generated by ...
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New Approaches to Photodynamic Therapy from Type I, II and III to Type IV Using One or More Photons
... two-photon excitation (2PE) of PSs in the NIR between 600 - 1100 nm (“tissue window”, Figure 4C) has been suggested as an innovative approach compared with conventional ...the excitation light in tissues ...
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Two-photon phosphorescence lifetime imaging of cells and tissues using a long-lived cyclometallated (NpyridylCphenylNpyridyl)-C-boolean AND-N-boolean AND Pt(II) complex
... two-photon excitation enables one to achieve the essential for life sciences sub-micron spatial resolution and NIR-excitation in the range of relative tissue ...two-photon excitation also requires a ...
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Nanoparticles for highly efficient multiphoton fluorescence bioimaging
... to multiphoton imaging is the investigation of the photon- order excitation dependence and how it is affected by the excitation ...the multiphoton excitation power (intensity) for each ...
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Wide field multiphoton imaging through scattering media without correction
... out-of-focus excitation in line-scanning multiphoton microscopy ...efficient multiphoton excitation when compared to stan- dard point-focusing where spatial speckle greatly reduces the photon ...
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Wide-field multiphoton imaging through scattering media without correction
... out-of-focus excitation in line-scanning multiphoton microscopy ...efficient multiphoton excitation when compared to stan- dard point-focusing where spatial speckle greatly reduces the photon ...
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Single and multi photon excited fluorescence from serotonin complexed with B cyclodextrin
... FIGURE 4 Fluorescence (hyperluminescence) lifetimes of the biexponential decay from serotonin (1 mmol dm -3 ) on multiphoton excitation at 750 nm and pH 7 with increasing β-cyclodextrin concentration. ...
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Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds
... and horseradish peroxidase (HRP) [23, 24]. When the cells are cultured in hydrogel-based scaffolds, they are grown in- side the hydrogel in 3D. Especially, gelatin-based hydrogels have excellent biocompatibility and ...
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New Approaches to Photodynamic Therapy from Type I, II and III to Type IV Using One or More Photons
... two-photon excitation (2PE) of PSs in the NIR between 600 - 1100 nm (“tissue window”, Figure 4C) has been suggested as an innovative approach compared with conventional ...the excitation light in tissues ...
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Innovations of Wide-Field Optical-Sectioning Fluorescence Microscopy: Toward High-Speed Volumetric Bio-Imaging with Simplicity
... single-point-scanning multiphoton excitation microscopy ...of-focus excitation of our time-multiplexed system and a non-time-multiplexed system, we chose a microlens array where the aperture formed ...
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Near infrared multiphoton induced generation and detection of hydroxyl radicals in a biochemical system
... have a slope of 5.07 ± 0.16 in the log-log plot indicating an overall 5-photon process at 750 nm, compared with a 6-photon excitation reported at 830 nm [27]. The power dependence of the weaker emission at 510 nm ...
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Use of confocal and multiphoton microscopy for the evaluation of micro-optical components and emitters
... Since the introduction of high resolution reliable commercial confocal microscopes in the mid 1980’s they have become one of the key tools for life science researchers, enabling routine three dimensional visualisation of ...
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Resonance enhanced multiphoton ionization spectroscopy oflaser ablated copper atoms
... LaVa source, where it flowed over the metal rod. The resulting hot, metal-containing plasma is collisionally cooled by the argon as it passes along a 1.5 mm diameter exit channel (length 20 mm) before expansion into the ...
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Modal Analysis of a Non-rotating Inflated Tire using Experimental and Numerical Methods
... The rim was hanged from a metal structure in free-free condition by a flexible sling. This experiment is performed twice in to different condition with two accelerometers. In the first condition an excitation ...
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A generic π* shape resonance observed in energy dependent photoelectron angular distributions from two colour, resonant multiphoton ionization of difluorobenzene isomers
... Continuum multiple scattering Xα calculations reproduce this behaviour well, and allow the vi- sualisation of the continuum shape resonances. The resonances are unusually narrow in energy (<1 eV), but nevertheless ...
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Multiphoton Confocal Microscopy (in vivo Imaging) in the Study of Early Response of Macrophages/Microglia in Damaged Midbrain of Juvenile Chum Salmon Oncorhynchus keta
... used multiphoton confocal microscopy for the in vivo study of early response of macrophages/microglia in the damaged midbrain of juvenile chum salmon Oncorhynchus ...using multiphoton confocal microscopy in ...
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