Multiplex Ligation-Dependent Probe Amplification
Homozygous deletion of TNFRSF4, TP73, PPAP2B and DPYD at 1p and PDCD5 at 19q identified by multiplex ligation-dependent probe amplification (MLPA) analysis in pediatric anaplastic glioma with questionable oligodendroglial component
... Samples and clinical-pathological data were obtained according to the hospital ethics committee procedures. DNA was isolated from frozen tumor and peripheral blood using the Wizard Genomic DNA purification kit (Promega). ...
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Rapid screening of innate immune gene expression in zebrafish using reverse transcription multiplex ligation dependent probe amplification
... Multiplex Ligation-dependent Probe Amplification (MLPA) is a high resolution method to establish the copy number of around 40 nucleic acid sequences in a one-tube reaction ...
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Comprehensive chronic lymphocytic leukemia diagnostics by combined multiplex ligation dependent probe amplification (MLPA) and interphase fluorescence in situ hybridization (iFISH)
... These aberrations were either detected applying cytogen- etics and/or interphase fluorescence in situ hybridization (iFISH) [3] or more recently multiplex ligation-dependent probe ...
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Promoter hypermethylation in male breast cancer: analysis by multiplex ligation dependent probe amplification
... methylation-specific multiplex ligation-dependent probe amplification; PAX5: paired box 5; PAX6: paired box 6; PCR: polymerase chain reaction; PR: progesterone receptor; PTEN: ...
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Investigation of selected genomic deletions and duplications in a cohort of 338 patients presenting with syndromic obesity by multiplex ligation-dependent probe amplification using synthetic probes
... required. Multiplex ligation-dependent probe amplification (MLPA) enables the simultaneous analysis of multiple targeted loci in a single test, and serves as an important screening tool ...
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Rapid Detection of Hepatitis B Virus Variants Associated with Lamivudine and Adefovir Resistance by Multiplex Ligation Dependent Probe Amplification Combined with Real Time PCR
... a multiplex ligation-dependent probe real-time PCR (MLP-RT-PCR) method was developed to simultaneously detect lamivudine (LAM)- and adefovir (ADV)-resistant HBV mutants (those with the ...
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Identification of Salmonella enterica Serovar Typhi Genotypes by Use of Rapid Multiplex Ligation Dependent Probe Amplification
... standardized multiplex ligation-dependent probe amplification (MLPA) genotyping scheme targeting 11 key phylogenetic markers of the ...
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Multiplex ligation-dependent probe amplification and array comparative genomic hybridization analyses for prenatal diagnosis of cytogenomic abnormalities
... [6,7]. Multiplex ligation-dependent probe amplification (MLPA) was validated as a rapid, cost-effective and multi- allelic approach for prenatal screening of common aneu- ploids, ...
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Altered Methylation at MicroRNA-Associated CpG Islands in Hereditary and Sporadic Carcinomas: A Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach
... Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assays were developed for 11 miRNA loci that were chosen because all could be epigenetically regulated ...
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Quantitative copy number analysis by Multiplex Ligation dependent Probe Amplification (MLPA) of BRCA1 associated breast cancer regions identifies BRCAness
... a Multiplex Ligation-dependent Probe Amplification (MLPA) ...on amplification and relative quantification of the ligated adjacent probes, which can target up to 50 differ- ent ...
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Evaluation of multiplex ligation dependent probe amplification (MLPA) for identification of acute lymphoblastic leukemia with an intrachromosomal amplification of chromosome 21 (iAMP21) in a Brazilian population
... [13]. Multiplex ligation-dependent probe amplification (MLPA) assays represent a quick and cost effective alternative to array based techniques allowing screening of large cohorts for ...
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Multiplex ligation-dependent probe amplification workflow for the detection of submicroscopic chromosomal abnormalities in patients with developmental delay/intellectual disability
... of multiplex ligation-dependent probe amplification (MLPA) and array based comparative genomic hybridization (arrayCGH) techniques for the accurate assessment of copy number changes at ...
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Subtelomeric multiplex ligation-dependent probe amplification as a supplement for rapid prenatal detection of fetal chromosomal aberrations
... whether Multiplex Ligation-dependent Probe Amplification (MLPA) using subtelomeric probe mixes (P036-E2 and P070-B2) is a reliable method for rapid detection of fetal chromosomal ...
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Multiplex ligation dependent probe amplification versus karyotyping in prenatal diagnosis: the M A K E study
... Background: In the past 30 years karyotyping was the gold standard for prenatal diagnosis of chromosomal aberrations in the fetus. Traditional karyotyping (TKT) has a high accuracy and reliability. However, it is labor ...
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Same Day Subtyping of Campylobacter jejuni and C coli Isolates by Use of Multiplex Ligation Dependent Probe Amplification–Binary Typing
... A multiplex ligation-dependent probe amplification-binary typing (MBiT) assay was devel- oped for subtyping Campylobacter jejuni and Campylobacter ...
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Breast carcinomas with low amplified/equivocal HER2 by Ish: potential supporting role of multiplex ligation-dependent probe amplification
... Multiplex Ligation-dependent Probe Amplification (MLPA) (MRC-Holland, Amsterdam, The Netherlands) is a semi quantitative technique which needs only minute quantities of DNA isolated ...
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Can we rely on the multiplex ligation-dependent probe amplification method (MLPA) for prenatal diagnosis?
... out multiplex ligation-dependent probe amplification (MLPA) for the rapid detection of aneuploidies of chromosomes X, Y, 13, 18 and 21 in amniotic fluid cells (10, ...the ...
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Clinical experience with multiplex ligation-dependent probe amplification for microdeletion syndromes in prenatal diagnosis: 7522 pregnant Korean women
... the multiplex ligation-dependent probe amplification (MLPA) P245 assay used for prenatal diagnosis in cases with unusual ultrasonographic findings or specifically where parents wanted ...
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Multiplex ligation dependent probe amplification (MLPA) for rapid distinction between unique sequence positive and negative marker chromosomes in prenatal diagnosis
... of Multiplex Ligation Dependent Probe Amplification (MLPA) (MRC Holland, Amsterdam, The Netherlands) as an alternative approach for identification of euchromatic ...
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Detection of Partial Deletions of Y-chromosome AZFc in Infertile Men Using the Multiplex Ligation-dependent Probe Amplification Assay
... MLPA probe design for this probemix was complicated by the presence of an inverted tandem duplication in AZFc which contains many of the important spermatogenesis ...
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