Multiplex PCR for detecting mobilised P-elements
Utility of a Multiplex PCR Assay for Detecting Herpesvirus DNA in Clinical Samples
... by PCR. A typical gel of the multiplex PCR is shown in Fig ...of PCR, EHV-4 was added to each test sample by incorporating it into the NP-40 used for ...
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Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
... 2.5. Thermal profile and optimization conditions Separate reaction mixtures were carried out for each real- time RT-PCR with parameters adjusted for each virus target. The amplification reaction was performed in a ...
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Multiplex PCR Method of Detecting Pork to Guarantee Halal Status in Meat Processed Products
... Regency. PCR multiplex was a PCR technique that used several primers together in one reaction to amplify several target ...pork. Multiplex PCR testing on meatball samples showed that ...
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A Multiplex PCR Approach for Detecting Dual Infections and Recombinants Involving Major HIV Variants
... for detecting MO recombinants, as demonstrated for the RBF208 recombinant, which was identified as an HIV-1/O strain (but consistent with its recombination pattern) rather than as a ...
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Serotyping Streptococcus pneumoniae by Multiplex PCR
... for detecting epidemiologically important serotypes present in the proposed 11-valent conjugate ...by multiplex PCR. Capsular types identified by the PCR method invariably produced results ...
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Rapid Diagnosis of Leptospirosis by Multiplex PCR
... In this report, we described the development of an mPCR for the rapid detection of leptospiral infection based on the amplification of 2 genes: 16S rRNA gene and LipL32 gene. The mPCR used in this study offers further ...
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... number contains a level of uncertainty exacerbated by differences among virus strains, tissue culture conditions, and the length of time that the virus was stored. In addition, viral RNA is labile and is subject to rapid ...
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Multiplex PCR for Detection and Typing of Porcine Circoviruses
... though PCR was reported to be a useful tool for detecting PCV in clinical specimens from naturally and experimentally infected pigs (12, 14, 25), an investigation such as the one described herein was needed ...
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Multiplex PCR: Optimization and Application in Diagnostic Virology
... in PCR technology, however, may facilitate the development of multiplex ...start PCR (8, 53). Thorough evaluation and validation of new multiplex PCR procedures is ...by PCR ...
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DNA Differential Diagnosis of Taeniasis and Cysticercosis by Multiplex PCR
... of multiplex/nested PCR may be somewhat lower in comparison with that of copro-antigen detection test; however, PCR diag- nosis has an advantage in its ability to differentiate human taeniid cestode ...
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Multiplex PCR for Simultaneous Detection of Virulence Genes in Escherichia coli
... a multiplex PCR method was a tedious but necessary step towards minimizing the cost and labor involved in detecting multiple targets ...The multiplex PCR technique was improved by ...
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Multiplex PCR for Typing and Subtyping Influenza and Respiratory Syncytial Viruses
... of detecting and subtyping influenza virus and RSV, preferably in a single step, and that is not dependent on the presence of infectious ...sought. Multiplex PCR should be able to accomplish these ...
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Troubleshooting for PCR and multiplex PCR
... (multiplexed) locus individually, using an annealing temperature lower than usual. Compare the unspecific products for each locus tested with the unspecific products seen when running the multiplex PCR. ...
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QuantiFast Multiplex PCR Handbook
... For reverse transcription, we recommend using the QuantiTect Reverse Transcription Kit. The kit provides a fast and convenient procedure, requiring only 20 minutes to synthesize first-strand cDNA and eliminate genomic ...
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QIAGEN Multiplex PCR Handbook
... Special Multiplex PCR Applications Sensitive multiplex PCR assays PCR is an exquisitely sensitive technique that can be used to amplify and detect even a single copy of a nucleic acid ...
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... employ multiplex-PCR techniques or reverse transcription (RT)- polymerase chain reaction (PCR) for detection of several infectious agents in co-infected patients simultaneously (7, ...
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BacReady TM Multiplex PCR System
... TM Multiplex PCR System with enzyme (L00203) contains BR-A buffer and 2X PCR ...DNA. PCR premix contains PCR buffer (BR- B buffer), dNTP, Mg 2+ and “HotStart” Script TM DNA polymerase ...
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Platinum Multiplex PCR Master Mix
... Note: A 4% agarose E-gel is recommended. If the template DNA is homozygous for the amplified target, use only the primers that generate a single band with the correct size for multiplex PCR. If a primer ...
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Cryptococcus species identification by multiplex PCR
... The multiplex PCR identifications were in 100% agreement with those obtained through ...The PCR was more accurate than the CGB test in six ...of PCR is about six days faster than that of the ...
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Preserving Mobilised Culture
... As networked publics (Friedland, Hove, & Rojas, 2006) proliferate, traditionally marginalised digital citizen documentation is increasingly ubiquitous. It functions as sousveillance, 1 is included in mainstream news ...
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