murine leukemia virus vector
Enhancers Are Major Targets for Murine Leukemia Virus Vector Integration
... MLV vector are also valuable for the gene therapy ...MLV vector in CD34 ⫹ ...the murine xenograft ...the vector during ex vivo transduction of CD34 ⫹ ...MLV vector integration sites ...
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The Ataxia Telangiectasia-Mutated and Rad3-Related Protein Is Dispensable for Retroviral Integration
... titis virus G protein (VSVG) ...mutant virus was constructed with ...The murine leukemia virus vector was produced as de- scribed above for lentivirus vectors except the ...
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Gene transfer into mammalian cells by a Rous sarcoma virus-based retroviral vector with the host range of the amphotropic murine leukemia virus.
... the vector into cultured chicken embryo fibroblasts (CEF). High- titer virus stocks can be prepared simply by passaging trans- fected cells and allowing the virus to ...competent virus, RCAS ...
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Diminished Potential for B-Lymphoid Differentiation after Murine Leukemia Virus Infection In Vivo and in EML Hematopoietic Progenitor Cells
... the murine lymphohematopoi- etic progenitor cell line EML, a bone marrow-derived, stem cell factor-dependent progenitor cell line capable of differen- tiation along erythroid, myeloid, and lymphoid ...retrovirus ...
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Identification of Directly Infected Cells in the Bone Marrow of Neonatal Moloney Murine Leukemia Virus-Infected Mice by Use of a Moloney Murine Leukemia Virus-Based Vector
... While the experiments reported here identified osteoclasts as at least one cell type initially infected by M-MuLV in the bone marrow after i.p. inoculation, there were limitations to the conclusions. First, we did not ...
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Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus.
... 3 Construction of a Mammalian Transducing Vector from the Genome of Moloney Murine Leukemia Virus ELI GILBOA,* MARGRET KOLBE, KEVIN NOONAN, AND RAJU KUCHERLAPATI Department of Biochemica[r] ...
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Foamy Virus Envelope Glycoprotein-Mediated Entry Involves a pH-Dependent Fusion Process
... with murine leukemia virus or FV vector pseudotypes in the presence of various lysosomotropic ...stomatitis virus glycoprotein G (VSV-G)-mediated uptake, FV Env-mediated entry was ...
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High rate of genetic rearrangement during replication of a Moloney murine leukemia virus-based vector.
... PG13 cell clones containing MSTK vector proviruses were established by infection with virus produced in GP+E-86 helper cells at low multiplicity of infection and used in the forward muta[r] ...
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Construction and Characterization of a Hybrid Mouse Mammary Tumor Virus/Murine Leukemia Virus-Based Retroviral Vector
... the murine leukemia virus (MLV) U3 region, carrying the two 72-bp repeat enhancer elements, were deleted, creating self-inactivating ...the vector have also been ...the vector, the ...
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Nonreciprocal Pseudotyping: Murine Leukemia Virus Proteins Cannot Efficiently Package Spleen Necrosis Virus-Based Vector RNA
... Viral RNAs were isolated to examine the packaging of these vector RNAs in viral particles generated by PG13 (Fig. 4B and C). For each set of experiments, supernatants from pools of PG13 cells infected with JS12, ...
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Influence of DNA Methylation and Core Binding Factors in Expression of Leukemia Virus in Hemapoietic Stem Cells Manoj G. Tyagi 1* and Aniket Kumar1
... Moloney murine leukemia virus (MLV), such as the MFG vector (Walsh et ...of murine Moloney leukemia virus provirus DNA in virus-infected ES ...
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Replacement of Murine Leukemia Virus Readthrough Mechanism by Human Immunodeficiency Virus Frameshift Allows Synthesis of Viral Proteins and Virus Replication
... By using the two-step PCR extension method (12) schema- tized in Fig. 1, the HIV-1 frameshift signal, encompassing both the slippery heptamer and stem-loop, was thus inserted at the junction of the gag and pol gene in ...
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Functional Interplay Between Murine Leukemia Virus Glycogag, Serinc5, and Surface Glycoprotein Governs Virus Entry, with Opposite Effects on Gammaretroviral and Ebolavirus Glycoproteins
... Measurement of MLV entry into susceptible cells. In order to localize the block more precisely, we needed a way to monitor the entry of MLV particles into new host cells. One such assay has been described by Kolokotsov ...
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Transfer of Primer Binding Site-Mutated Simian Immunodeficiency Virus Vectors by Genetically Engineered Artificial and Hybrid tRNA-Like Primers
... MLV-based vector (B) are ...MLV-based vector pPBS-X2 (28) into the packaging cell line BOSC 23. Vector titers in the supernatant of the transfected cells were determined on NIH 3T3 ...
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TM domain swapping of murine leukemia virus and human T-cell leukemia virus envelopes confers different infectious abilities despite similar incorporation into virions.
... panel, virus particles released into the medium were ultracentrifuged at 24,000 rpm through a 20% sucrose cushion prior to solubilization in lysis ...panel, virus particles released in the medium were ...
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Rescue of Murine Sarcoma Virus from a Sarcoma-Positive Leukemia-Negative Cell Line: Requirement for Replicating Leukemia Virus
... 690-694 Copyright © 1971 American Society for Microbiology Rescue of Murine Sarcoma Virus from a SarcomaPositive Leukemia-Negative Cell Line: Requirement for Replicating Leukemia Virus R[r] ...
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Temperature-sensitive viral RNA expression in Moloney murine sarcoma virus ts110-infected cells.
... The splicing defect of the tsllO Moloney murine sarcoma virus viral RNA in 6m2 cells cannot be complemented by acute Moloney murine leukemia virus superinfection, since no 3.5-kb tslO RN[r] ...
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Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses
... The viruses were derived from a pBR328 subclone of lSL3-95 (19), T464, containing a functional SL3-3 provirus surrounded by sequences of genomic DNA. A deletion mutant, T464(DPvuI), lacking the 59 LTR region and part of ...
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Proviral genome of radiation leukemia virus: molecular cloning of biologically active proviral DNA and nucleotide sequence of its long terminal repeat.
... per ml and inoculated as 50-,lI samples intrathymically Radiation leukemia virus RadLV is an in vivo-passaged murine leukemia virus MuLV complex, isolated from thymic lymphomas of X-ray-[r] ...
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Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome
... monkey virus (MPMV), human foamy virus (HFV), retic- uloendotheliosis virus strain A (REV-A), human T-cell leukemia virus type 1 (HTLV-1), feline leukemia virus (FeLV), ...
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