Mutational analysis of the EBPI binding site
Mutational analysis of a catalytically important loop containing active site and substrate-binding site in Escherichia coli phytase AppA
... active site loop to the AppA activity, alanine and glycine scanning mutagenesis was ...mutation site against mutant activity revealed that the loop residues located closer to the substrate have greater ...
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Mutational analysis of a proposed structural element in the SRP9/14p binding site of Schizosaccharomyces pombe SRP RNA
... 8 suggests that, specifically, a single-stranded purine is required at this position (lia o et a/., 1002 ). The goal of my senior thesis research is to further characterize the SRP0/14p binding site as the ...
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Transcription Factor Binding Site Analysis
... Several of these differentially methylated sites were validated by bisulfite pyrosequencing. For the Decision Tree classifiers predicting transcription factor target genes, Shyr C, Pearson Correlation and Spearman ...
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Mutational Analysis of the Bovine Hepacivirus Internal Ribosome Entry Site
... 39). Mutational analysis of the seed match site revealed only a minor influence of miR-122 abundance and properties of the binding site on translational ...
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Mutational Analysis of the GB Virus B Internal Ribosome Entry Site
... entry site (IRES) bounded at its 5 ⴕ end by structural domain II, a location analogous to the 5 ⴕ limit of the IRES in both the HCV and pestivirus 5 ⴕ ...NTRs. Mutational analysis confirmed the ...
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Inhibition of Viral Replication by Ribozyme: Mutational Analysis of the Site and Mechanism of Antiviral Activity
... The use of nucleic acids to achieve the selective inhibition of gene expression has been broadly investigated by the use of antisense, triplex, ribozyme, and RNA interference strategies (for reviews, see references 1, ...
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Structural analysis of the biliary glycoprotein (BGP) binding site
... an d C term ini are situ ated at op p o site en d s of the stru ctu res. An unusual quasi a-helix connects p-strands C an d D. These structures w ere found to be rem arkably like the im m unoglobulin fold, in ...
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Mutational and Metal Binding Analysis of the Endonuclease Domain of the Influenza Virus Polymerase PA Subunit
... specific binding of the cap structure and an endonuclease ...cap-binding site is situated in the central part of the PB2 subunit and that the endonuclease activity is situated in the N-terminal ...
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Analysis of the receptor-binding site of murine coronavirus spike protein.
... receptor-binding site of rhinoviruses is thought to form a so-called canyon, a small concave region too small for im- munoglobulin molecules to gain access ...active site but instead probably ...
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Mutational Analysis of the Binding Pockets of the Diketo Acid Inhibitor L-742,001 in the Influenza Virus PA Endonuclease
... proposed binding mode of the endonuclease inhibitors in the con- text of the entire viral polymerase complex, as present in influenza virus-infected ...in binding and anti- viral activity of L-742,001, here ...
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Phosphorylation of AKT: a Mutational Analysis.
... active site [35]. A mutation of this site is therefore expected to have a negative effect on AKT functions, and this is confirmed in the transformation and signaling studies (Table 1, ...
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Mutational analysis of the human immunodeficiency virus type 1 env gene product proteolytic cleavage site.
... The structural requirements for proteolytic cleavage of the human immunodeficiency virus type 1 env gene product, gpl60, to gpl20 and gp4l have been assessed by specific mutagenesis of t[r] ...
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Expression of the varicella-zoster virus origin-binding protein and analysis of its site-specific DNA-binding properties.
... It was previously shown, by using a protein A fusion protein, that the product of gene 51 is a site-specific DNA-binding protein which binds to sequences within the VZV origin (Stow et a[r] ...
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Transient expression and mutational analysis of the rotavirus intracellular receptor: the C-terminal methionine residue is essential for ligand binding.
... Use of the T7-vaccinia virus transient expression system to deliver a range of mutant forms of NS28 has provided further evidence that this protein acts as the intracellular receptor for[r] ...
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Analysis of site-directed mutants of the FX-binding region of photosystem I and second site revertants in Chlamydomonas reinhardtii
... on the "borderline" o f being com petent in photosynthesis. O ne o f the factors w hich determ ines the rate o f electron transfer is the distance betw een carriers. A \ has not been exactly located in the current 4Â ...
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Ligand docking and binding site analysis with pymol and autodock in elimination of lymphatic filariasis
... Analysis of docking results (Fig. 5): Docking poses generated by the docking programs can be directly loads into PYMOL through the plugin. Poses for multiple ligands may be handled simultaneously using an ...
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The degree of intratumor mutational heterogeneity varies by primary tumor sub-site
... Statistical analysis Due to the small sample size, medians and ranges are reported for continuous patient and tumor ...chi-square analysis was performed to determine if dichotomous mutation levels are ...
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Mutational Analysis of the Lassa Virus Promoter
... Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany Received 29 June 2006/Accepted 19 September 2006 The promoter sequences directing viral gene expression and genome ...
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Mutational Analysis of the Drosophila homothorax Gene
... The analysis of hth mutations demonstrates that the homeodomain of HTH is not required for nuclear localization of EXD and that the MH domain-containing first 240 residues are sufficient for nuclear localization ...
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Functional analysis of the secondary HIV 1 capsid binding site in the host protein cyclophilin A
... ary site mutants without folding defects to determine whether its interaction with the wild type CA lattice of authentic viral cores was impaired compared to wild type ...The binding affinity and the number ...
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