N-terminal amino acid sequence
Isolation by high-performance liquid chromatography and partial characterization of a 57,000-dalton herpes simplex virus type 1 polypeptide.
... Further characterization was achieved by amino acid analysis, determination of the N-terminal amino acid sequence in a gas-phase sequencer and by reaction with monoclonal antibodies dire[r] ...
6
Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase.
... Ultimately, the reading frame assignments were made by the identification of the N-terminal amino acid sequence of the isolated proteins and comparing these with the sequences predicted [r] ...
6
Mycobacterium tuberculosis HBHA Protein Reacts Strongly with the Serum Immunoglobulin M of Tuberculosis Patients
... Identification and characterization of serologically active mycobacterial antigens are prerequisites for the development of diagnostic reagents. We examined the humoral immune responses of active tuberculosis (TB) ...
7
Isolation of a lentivirus from a macaque with lymphoma: comparison with HTLV-III/LAV and other lentiviruses.
... The N-terminal amino acid sequence of the purified p28 protein of MnIV was determined by Edman degradation and compared with the N-terminal sequences of the major gag proteins of other l[r] ...
8
Proteome analysis of soybean leaves, hypocotyls and roots under salt stress
... The sequence shown is the N-terminal amino acid sequence determined by protein sequencing; c) Accession No, Accession number; d) ...SC, Sequence coverage by peptide mass ...
15
Biochemical and immunological characterization of the duck hepatitis B virus envelope proteins.
... In serum, the pre-S proteins were shown to exist predominantly in DHBV-DNA-free particles associated with a 17-kilodalton protein which, by N-terminal amino acid sequence analysis, was s[r] ...
6
Secretion of the mycobacterial 19-kilodalton protein by Escherichia coli, a novel method for the purification of recombinant mycobacterial antigens.
... codon. N-terminal sequence analysis of the purified protein revealed that the signal peptide of MPB70 was cleaved by an endopeptidase of ...at amino acid residue ...
7
Antigenic structure of rabies virus glycoprotein: ordering and immunological characterization of the large CNBr cleavage fragments.
... To map the CNBr peptides within the amino acid sequence of the glycoprotein deduced from the glycoprotein gene cDNA sequence, we determined the N-terminal sequence of each peptide.. Fluo[r] ...
8
Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication
... the N-terminal amphipathic helix in viral protein 2C was replaced by orthologous sequences from other picornaviruses or from the NS5A protein from hepatitis C virus ...seven amino acid changes ...
11
The Hantaan virus M-segment glycoproteins G1 and G2 can be expressed independently.
... Both the Uukuniemi virus and HTV M segments contain a hydrophobic N-terminal sequence that can serve as a signal sequence for glycoproteins, a hydrophobic amino acid stretch close to the[r] ...
8
Role of the Rous Sarcoma Virus p10 Domain in Shape Determination of Gag Virus-Like Particles Assembled In Vitro and within Escherichia coli
... of amino acid residues from the N terminus, using the standard numbering for the Prague C strain of RSV ...wild-type sequence at this location. The 25-aa sequence corresponding to the ...
9
Nucleotide sequence and protein analysis of a complex piscine retrovirus, walleye dermal sarcoma virus.
... nucleotide sequence of a DNA clone of WDSV, the N-terminal amino acid sequences of the major proteins, and the start site for ...long terminal repeat is 590 bp in length, with ...
12
Purification, crystallization and cloning of tributyrin esterase from Lactococcus : a thesis presented in partial filfillment of the requirements for the degree of Master of Technology in Biotechnology in the Institute of Molecular Biosciences at Massey University, New Zealand
... 1.4.1.2 Primary Amino Acid Sequence of Tributyrin Esterase Comparison of all the known N-terminal sequences of esterases from starter bacteria Table 1.2 reveals a striking similarity bet[r] ...
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Synthesis and evaluation of a new radiolabeled bombesin analogue for diagnosis of GRP receptor expressing tumors
... It has been shown that 7-14 amino acid sequence of BN with amidated c-terminus is necessary for receptor binding affinity and n-terminal of peptide can be used for labeling (7).. The[r] ...
9
Domains for protein-protein interactions at the N and C termini of the large subunit of bacteriophage lambda terminase.
... The derived amino acid sequence was found to be quite different from the A prohead binding domain for the C-terminal 32 amino acids, but the predicted secondary struc[r] ...
8
Glutamate dehydrogenase, its role, regulation and characterisation in carrot cell suspension cultures
... However, this acceptance came into question in the early 1970's. The relatively high in vitro Km of GDH for ammonia indicates a low affinity of the enzyme for its substrate. Ammonia at the higher concentrations needed ...
210
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications.
... random amino acids (excluding cysteine) were introduced at Loops 1 and 2 by replacing four and three amino acid codons, respect- ively, by using codon-selected semi-trinucleotide cassette syn- ...the ...
11
Investigation of rhizobial dehalogenase control system in dehalogenase gene expression
... 3.3 Nucleotide sequencing and oligodeoxyribonucleotide synthesis Plasmid DNA was prepared using the Wizard Kit (Promega). Sequencing of both DNA strands were carried out using Applied Biosystem-ABI PRISM 377 (BigDye® ...
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Interspecific comparisons of the structure and regulation of the Drosophila ecdysone-inducible gene E74.
... Amino acid sequence comparisons of the E74A protein reveal a highly conserved C-terminal region that is rich in basic amino acid residues and which has been propo[r] ...
9
Structural and biochemical studies on the biosynthetic pathways of cyanobactins
... and N-terminal cleavage (PatApr) because the leader sequence must be removed to allow the N-terminus of the core peptide to bond directly with its C-terminus ...
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