Non Invasive method for SIngle Molecule Analysis for cell Metabolism
Comparing Invasive and Non-Invasive of Isolated Shigella flexneri by Electron Microscopy of Cell Culture, SDS-PAGE and Congo Red Method
... compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye ...Phenotype ...
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Single Cell Tracking Reveals Antibiotic Induced Changes in Mycobacterial Energy Metabolism
... key molecule of cell physiology, but despite its importance, there are currently no methods for monitoring single-cell ATP fluctuations in live ...energy metabolism, because there is a ...
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From single cell to single molecule: elucidation of the intracellular dynamics of the Hypoxia Inducible Factor (HIF)
... one cell, one has to track the nucleus through the time ...BAC cell line, finding a spectrally suitable, non-toxic nuclear marker that will last the duration of the experiment (>24 h) proved ...
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Non invasive, spatio temporal gait analysis for sprint running using a single camera
... Using optimised system setups (i.e. higher and lower image segmentation thresholds), the Gait Analyser identified 100% of foot contacts: 95% LOA for X and Y direction foot contacts were 44.8 ± 232.9 and 44.4 ± 119.7 mm ...
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An Enrichment Method of Cell-free Fetal DNA from Mothers in the 11th Week of Pregnancy; On The Way of Non-invasive Prenatal Diagnosis of Beta-thalassemia as a Single Gene Disorder
... of cell free fetal DNA in maternal plasma has shown many ...However non-invasive prenatal testing (NIPT) for single gene disorders is straggling [1] Thalassemia has been considered as disease ...
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Comparison of the Automatic Non-invasive Express Screening Analyser (ANESA) for Clinical Analytical Parameters
... blood cell and peroxisome proliferator-activated (PPAR) ...on metabolism of amino acids, phospholipids and cholesterol, and water ...The method in whole is widely described in monograph ...
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Noninvasive Prenatal Testing for Fetal Chromosomal Abnormalities Using Massively Parallel Sequencing: Clinical Experience from 7910 Korean Pregnancies
... Traditional invasive methods of fetus prenatal screening, ...novel Non-Invasive Prenatal Test (NIPT) method to analyze fetus aneuploidy using cell-free DNA (cfDNA) in the plasma of ...
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Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems
... the cell and the synthetic potentiality of the ...possible method. Using the microspectral analysis of cells and a large set of luminescent dyes-labels, other useful char- acteristic parameters can ...
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Non invasive and non destructive measurements of confluence in cultured adherent cell lines
... output method to ...‘ cell screen ’ which requires sample manipulation and use of an inverse microscope equipped with 10 objective and a back-illuminated charge-coupled device (CCD) camera which is operated ...
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Localization and abundance analysis of human lncRNAs at single cell and single molecule resolution
... visualize single lncRNA molecules directly inside of cells, we used an established protocol for single molecule RNA FISH [24], where we design 10 to 48 complementary DNA oligonucleotides, each 20 ...
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Nutrients and nipple aspirate fluid composition: the breast microenvironment regulates protein expression and cancer aetiology
... a greater number of epithelial cells, useful for efficient optical and electronic microscopy studies [29–31]. The value of NAF has been extensively described [12, 30, 31, 43, 56], referring to breast secretions as ...
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<p>Radiomic signature: a non-invasive biomarker for discriminating invasive and non-invasive cases of lung adenocarcinoma</p>
... among non-smokers, with a high incidence in women and younger ...pre- invasive lesions, including atypical adenomatous hyperplasia (AAH) and adenocarci- noma in situ (AIS); (b) minimally invasive ...
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3D Traction Forces in Cancer Cell Invasion
... In this study we use reconstituted collagen gels that form spontaneously during self-assembly of collagen fiber networks (Fig. S1a). At a collagen concentration of 2.4 mg/ml, these networks form a porous structure with ...
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Non-invasive prenatal testing for fetal chromosome abnormalities: review of clinical and ethical issues
... and invasive PD in order to filter out most of the screening false positives (added to a subset of women as for contingent screening); or 3) cfDNA screening might replace invasive PD if it is ever ...
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Kidney injury molecule-1 expression in human kidney transplants with interstitial fibrosis and tubular atrophy
... injury molecule-1 - HAVCR1) ID: Hs00273334_m1] according to the manufacturer’s ...comparative method where CT (threshold cycle) value is defined as the point where a statistically signifi- cant increase in ...
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Minimally invasive cell-seeded biomaterial systems for injectable/epicardial implantation in ischemic heart disease
... histological analysis of the LV wall 8 weeks after injection revealed formation of a layer of smooth muscle next to the remaining material, which showed protein expression related to a contractile phenotype, ...
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Non-destructive, mechanical method for measurement of plums' firmness
... The mechanical properties of materials are important from the point of view of a material’s susceptibility to dama- ge and the influence of various external factors during har- vest, transport, storage and processing ...
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The antineoplastic drug, trastuzumab, dysregulates metabolism in iPSC-derived cardiomyocytes
... In summary our transcriptome based approach of tras- tuzumab induced changes in iPSC-derived cardiomyo- cytes identified significant down-regulation of expression of genes associated with ischemic preconditioning and ...
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Test-retest analysis of a non-invasive method of quantifying [11C]-PBR28 binding in Alzheimer’s disease
... Although [ 11 C]-PBR28 is known to have a better signal-to-noise ratio and pharmacokinetic properties, compared to the first-generation TSPO tracer [ 11 C]-R- PK11195, its use in longitudinal studies of AD is limited by ...
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Thermal Decomposition Kinetics of Ursodeoxycholic Acid Drug Crystal
... thermal analysis was carried out on the as grown single crys- tals of ursodeoxycholic acid crystallized from gel growth ...to single crystal X-ray diffraction analysis for the structure ...
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