NS2B/NS3 protease
Dengue Virus NS2B/NS3 Protease Inhibitors Exploiting the Prime Side
... virus NS2B/NS3 ...NS2/NS3 protease is a promising target for inhibitor ...DENV protease active site, resulting in highly hydrophilic and nonspecific ...DENV protease binding ...
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Identification of Linked NS2B-NS3 Protease Conformation of the Zika Virus with Allosteric Bioactive Compound Antagonists
... Pharmacokinetic studies showed that NITD008 GI absorption is in low level in plasma when taken orally. But the value of this absorption can be enhanced by covalently linking isobutyrate acid to the 3' and 5' hydroxyl ...
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Identification of Residues in the Dengue Virus Type 2 NS2B Cofactor That Are Critical for NS3 Protease Activation
... 2 NS3 serine protease (protein database identifier 1BEF) (31) and the hepatitis C virus NS3/NS4A complex (protein database identifier 1JXP) (49) were obtained from the protein database (Brookhaven ...
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Inhibition of dengue NS2B NS3 protease and viral replication in Vero cells by recombinant retrocyclin 1
... short peptides can be produced in a soluble form in E. coli. However, it is often challenging to have correct di- sulphide bond formation in cysteine rich peptides. We have produced RC-1 as a soluble recombinant peptide ...
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Upregulation of signalase processing and induction of prM-E secretion by the flavivirus NS2B-NS3 protease: roles of protease components.
... viral protease and signalase cleavage sites are denoted below the ...the NS2B protein is ...into NS2B, and the open bar in the NJH cassette represents the JE virus-specific ...
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Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity.
... The data presented above suggested that the 40-aa domain of DEN4 NS2B from residues 1396 to 1435 might be the only region required for protease activity in cis other than the residues di[r] ...
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Formation of the flavivirus envelope: role of the viral NS2B-NS3 protease.
... Expression of the wild-type and mutated cassettes in vivo and in vitro. In general, expression using the vaccinia virus-T7 polymerase system (15), cell lysis, and immunoprecipitation were done as described earlier (46) ...
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Processing of the intracellular form of the west Nile virus capsid protein by the viral NS2B-NS3 protease: an in vitro study.
... Using Ci.t and C., translation products as molecular markers, we have observed processing of the intracellular form of the West Nile capsid protein by the viral protease in vitro both up[r] ...
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IDENTIFICATION OF INHIBITORS OF DENGUE VIRUS (DENV1, DENV2 AND DENV3) NS2B/ NS3 SERINE PROTEASE: A MOLICULAR DOCKING AND SIMULATION APPROACH
... against NS2b/NS3 serine protease of above three serotypes of DENV, whose infection results in DF using Autodock ...the NS2b/NS3 protease without changing the conformation of the ...
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Ligand-Bound Structures of the Dengue Virus Protease Reveal the Active Conformation
... (DENV-3) protease as well as the serine-protease inhibitor aprotinin bound to the same ...DENV protease. In the presence of the peptide, the DENV-3 protease forms the closed confor- mation in ...
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Structural Analysis and Molecular Modeling Studies of Fatty Acids and Peptides Binding with NS2B/NS3 Dengue Protease
... the NS2B/NS3 protease, the interactions and the glide score were relatively higher and the interaction with active site residues were favorable when the docking was carried out with 2M9P as target ...
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4-Thiazolidinone coumarin derivatives as two-component NS2B/NS3 DENV flavivirus serine protease inhibitors: synthesis, molecular docking, biological evaluation and structure–activity relationship studies
... virus NS2B/NS3 protease helps immensely in the prediction of their interaction ...DENV NS2B/NS3 protease was obtained from PDB (PBD ID: 2FOM), at a resolution of ...virus ...
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Flavanones: Potential antidengue targets in silico approach
... Flavanones are known to exhibit anti HIV [5], antimalarial [6], anticancer [7] and antibacterial [8] activities. Flavanones like rutin, fisetin are reported to exhibit antidengue activity [9]. Moreover flavones have ...
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Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3
... virus NS3 protease and therefore suggest that the residues selected in this study have probably a major function for substrate binding and ...virus NS3 protease, presumably as structural ele- ...
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Mutagenesis of the NS3 Protease of Dengue Virus Type 2
... DEN2 NS2B-NS3 sequence ...parental NS2B-NS3 sequence ...the NS3 mutant plasmids that are inherent to the parental pTM1-NS2B-NS3 construct are cor- rected except the silent ...
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Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3
... pestivirus NS3 conformation states with various protease cleav- age ...viral protease termini likely play a critical role to generate free proteases or modulate protease activity to facilitate ...
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Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures.
... The VP have a striking similarity in their ultrastructure to cryosections of special lysosomes which are multivesicular ma- jor histocompatibility complex (MHC) class II-containing com- partments and act as peptide ...
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NS3-4A of hepatitis C virus is a chymotrypsin-like protease.
... HCV protease NS3 is a good therapeutic target molecule for use in developing anti-HCV drugs since its activity is consid- ered to be essential for viral ...of NS3 and NS3-4A have been ...
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Generation and Characterization of a Hepatitis C Virus NS3 Protease-Dependent Bovine Viral Diarrhea Virus
... HCV NS3 and BVDV core fusion proteins. A single-chain HCV NS3 protease domain (H77 isolate), in which the NS4A cofactor peptide (GSVVIVGRIVLS) was fused in frame to the N terminus of the ...
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Nonstructural Protein 5A (NS5A) and Human Replication Protein A Increase the Processivity of Hepatitis C Virus NS5B Polymerase Activity In Vitro
... at 4°C (0.56 ml of saturated ammonium sulfate per 1 ml of cell extract), and the precipitates were pelleted by centrifugation at 10,000 ⫻ g for 30 min. The precipitates were resuspended in 4 ml of ice-cold buffer B (20 ...
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