nt, nucleotides
Advanced glycation end products increase retinal vascular endothelial growth factor expression
... previously (20). Briefly, the sense probe was prepared from a mouse plasmid DNA construct by cutting with BamHI and transcribed with T3, generating a riboprobe with a length of 650 nucleotides (nt). The ...
7
Characterization of the minute virus of mice P38 core promoter elements.
... between nt 171 to 180 [disrupting the P4 TATA element]); p385UTTLM4 (contains a linker insertion between nt 159 to 168 [disrupting the P4 Sp1 ele- ment]); and p385UTTID1 (contains a linker insertion between ...
8
Transmissible Gastroenteritis Coronavirus Genome Packaging Signal Is Located at the 5′ End of the Genome and Promotes Viral RNA Incorporation into Virions in a Replication-Independent Process
... in the genome within ORF1b, around 20 kb from the genomic 5= end. In addition, a 291-nt region in ORF1b, showing homology with the MHV sequence, was also described as the PS of BCoVs, belonging to the same genus, ...
12
Study on expression of lncRNA RGMB-AS1 and repulsive guidance molecule b in non-small cell lung cancer
... 200 nucleotides (nt) [11], are usually divided into ex- onic lncRNAs, intronic lncRNAs, intergenic lncRNAs and overlapping lncRNAs in accordance with their location relative to the protein-coding ...
7
Overlapping Local and Long-Range RNA-RNA Interactions Modulate Dengue Virus Genome Cyclization and Replication
... 300 nucleotides of the viral genome ...450 nt of the viral genome was probed with N-methylisotoic anhydride (NMIA) employing SHAPE (selective 2=-hydroxyl acylation ana- lyzed by primer extension) technology ...
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In Vivo Translation of the Triple Gene Block of Potato Virus X Requires Two Subgenomic mRNAs
... genomic nucleotides (nt) 4587 to 4945 as described previously (2), the D12K mutant contains a frameshift mutation resulting from the deletion of 11 nt between genomic nt 5240 to 5250, and the ...
5
Identification and transcriptional analyses of the UL3 and UL4 genes of equine herpesvirus 1, homologs of the ICP27 and glycoprotein K genes of herpes simplex virus.
... The UL3 ORF of 470 amino acids aa maps at nucleotides nt 4450 to 3038 from the long terminus, and its predicted 51.4-kDa protein product exhibits significant homology to the ICP27 alpha [r] ...
10
Characteristics and spread to the native population of HIV 1 non B subtypes in two European countries with high migration rate
... HIV-1 pol sequences 857 nucleotides nt long concatenated sequence fragments comprising codon 4 to 99 of the Protease gene and codon 30 to 226 of the Reverse transcriptase gene, were coll[r] ...
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RNA-RNA recombination in Sindbis virus: roles of the 3' conserved motif, poly(A) tail, and nonviral sequences of template RNAs in polymerase recognition and template switching.
... 24 nt but lacking the poly(A) tail is noninfectious; (iii) SIN RNAs which carry 3 * 64 nt or more without the poly(A) tail are infectious and regain their poly(A) tail in vivo; (iv) donor templates lacking ...
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Site-specific binding of the human cytomegalovirus IE2 86-kilodalton protein to an early gene promoter.
... Here we show that specific stimulation of this promoter by IE2 86 in transient-expression assays requires sequences located between nucleotides nt -113 and -58 relative to the transcript[r] ...
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Horizontal Transfer of a Retrotransposon from the Rice Planthopper to the Genome of an Insect DNA Virus
... 32 nucleotides [nt] in length), a small RNA class that was associated with Piwi-subfamily proteins, which might play essential roles in antiviral immunity of the mosquito Aedes aegypti, thereby providing a ...
16
Aichi Virus 2A Protein Is Involved in Viral RNA Replication
... ; nt 3896 to 3919) and G4170M (5 ⬘ -CGGCAACAGCAGCCG AGGCTGCGAT-3 ⬘ ; nt 4170 to 4147; the inserted nucleotide is underlined) were ...; nt 4056 to 4032) and NC-AA4057P (5 ⬘ -GCCGCTACCCACTTCGTCAGCTCCAT ...
5
Recombination in Tula Hantavirus Evolution: Analysis of Genetic Lineages from Slovakia
... To examine the evolution of Tula hantavirus (TUL), carried by the European common vole (Microtus arvalis and M. rossiaemeridionalis), we have analyzed genetic variants from Slovakia, the country where the virus is ...
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De Novo Initiation of RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase
... 98 nucleotides (nt) of HCV positive- strand RNA (11, 20, 22), was cloned into a transcription vector and the 3⬘ X-tail RNA (X-RNA) was transcribed in vitro using T7 RNA ...(341 nt) to produce ...
6
Polypyrimidine Tract-Binding Protein Binds to the Complementary Strand of the Mouse Hepatitis Virus 3′ Untranslated Region, Thereby Altering RNA Conformation
... of nucleotides (nt) 270 to 305 from the 3 ⴕ -UTR completely abolished subgenomic mRNA transcription without affecting minus-strand RNA synthesis ...350 nucleotides complementary to the 3 ⴕ -UTR of ...
7
Heterogeneity and evolution rates of delta virus RNA sequences.
... 5594 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To investigate the geographical divergence of delta virus RNA sequences, 868 nucleotides nt, including the delta an[r] ...
6
A Sequence-Independent, Unstructured Internal Ribosome Entry Site Is Responsible for Internal Expression of the Coat Protein of Turnip Crinkle Virus
... FIG 1 Deletion analysis of a region required for internal expression of the TCV CP. (A) Deletions generated in a 116-nt region immediately upstream of the CP ORF. Deleted regions are boxed in red. Construct ...
16
Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication
... Our finding of only limited conservation of the sequence in the 29-nt region in the M vRNA among our viral clones indi- cated that the nucleotide sequence in this region can be highly diverse without disrupting ...
5
Mutagenic Analysis of the 3′ cis-Acting Elements of the Rubella Virus Genome
... which nt 1 to 5 could be deleted, and the resulting virus was phenotypically ...of nucleotides between this nucleotide (a C) and the poly(A) could be tolerated ...
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Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus
... (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest ...
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