Nucleic acid sequence
Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... Nucleic acid sequence-based amplification (NASBA) is a sensitive, isothermal, transcription-based amplification system specifically designed for the detection of RNA ...
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Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples
... real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of human African trypanosomiasis ...on ...
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Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season
... and nucleic acid amplification techniques—nucleic acid sequence-based amplification (NASBA) and re- verse transcription-PCR (RT-PCR)—were applied to detect rhinoviruses ...
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Improved Detection of Rhinoviruses by Nucleic Acid Sequence Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains
... isothermal nucleic acid sequence-based amplification (NASBA) system was applied for the detection of rhinoviruses using primers targeted at the 5 ⴕ noncoding region (5 ⴕ NCR) of the viral ...
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RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples
... In the present study, the suitability of RNA amplification by nucleic acid sequence-based amplification (NASBA) for the detection of Chlamydia trachomatis infection was investigated. When comparing ...
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Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification
... Isothermal nucleic acid sequence-based amplification (NASBA) was applied to the detection of Mycoplasma pneumoniae. M. pneumoniae RNA prepared from a plasmid construct was used to assess the ...
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Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... isothermal nucleic acid sequence-based amplification (NASBA) assay was optimized to amplify viral RNA of all four dengue virus serotypes by a set of universal primers and to type the amplified ...
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Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification
... Alternatively, Nucleic Acid Sequence Based Amplification (NASBA) technology can be applied, which has as advan- tage above real-time PCR assays that it is fast to perform; ...
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Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
... quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania ...
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Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... isothermal nucleic acid sequence-based amplification (NASBA) was developed to detect Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella ...
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Development of novel fluorescent oligonucleotide probes for use in nucleic acid sequence analysis
... The solvents were removed in vacuo and the resulting oil dissolved in CH2Ch 200mL, washed with water 200mL, saturated sodium bicarbonate solution 200mL, brine 150mL and then dried Na2S04[r] ...
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Algorithms for nucleic acid sequence design
... this thesis may benefit from designing multiple unlinked leaves of a decomposition tree simultaneously. The challenge lies in that subproblem complexities are heterogeneous, making it difficult to preallocate resources ...
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Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA
... the amplification product was stored at ⫺20°C. Subsequently, 5 l of diluted amplification product was incubated for 30 min at 41°C with 20 l of a hybrid- ization mixture containing a biotinylated, target-specific ...
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iTriplet, a rule-based nucleic acid sequence motif finder
... contain a post-transcriptionally-added polyA tail that is important for many aspects of mRNA function. According to one bioinformatic study, 54% and 32% of genes in human and mouse, respectively, contain more than one ...
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PCR based assay for Mycoplasma hyopneumoniae
... The kit of claim 2 further comprising a probe compris ing the nucleotide sequence 5‘-GGTAGCCCITCCI'I'I‘GAGGT-3' SEQ ID NO: 3 that hybridizes to an ampli?ed target nucleic acid sequence r[r] ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... genomic sequence detection by reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the ...
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Comparison of PCR-generated fragments of the mce gene from Mycobacterium tuberculosis, M. avium, M. intracellulare, and M. scrofulaceum.
... ORF1 sequence fragment that did not cluster with the other ...only sequence whose amino acid transla- tion would create a truncated ...amino acid position 42. The amber codon is due to a ...
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Inherited Resistance to HIV-1 Conferred by an Inactivating Mutation in CC Chemokine Receptor 5: Studies in Populations with Contrasting Clinical Phenotypes, Defined Racial Background, and Quantified Risk
... The reports conflict on the effect of heterozy- gous CCR5-2 on HIV-1 susceptibility, and race and risk.. The nucleic acid sequence of CCR5-2 has been deposited in.[r] ...
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FASH: A web application for nucleotides sequence search
... Alignment Sequence Heuristics) is a web application, based on the Fast Fourier Transform, for finding remote homologs within a long nucleic acid ...query sequence and a long ...
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Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
... isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of ...
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