nucleic acid sequence-based amplification
Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season
... and nucleic acid amplification techniques—nucleic acid sequence-based amplification (NASBA) and re- verse transcription-PCR (RT-PCR)—were applied to detect ...
6
Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... isothermal nucleic acid sequence-based amplification (NASBA) assay was optimized to amplify viral RNA of all four dengue virus serotypes by a set of universal primers and to type the ...
5
Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia
... real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) ...
8
Diagnostic Value of Monitoring Human Cytomegalovirus Late pp67 mRNA Expression in Renal Allograft Recipients by Nucleic Acid Sequence Based Amplification
... Although amplification of the viral ge- nome by PCR proved to be a highly sensitive technique (up to 100%), it does not necessarily correlate with active infection, due to the possible amplification of ...
6
Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... pneumoniae amplification assays. For isothermal nucleic acid sequence-based amplification (NASBA), 16S rRNA has obvi- ously been used as a target, and for PCR, the 16S rRNA genes ...
6
Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples
... real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of human African ...
6
Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis
... of nucleic acid sequence-based amplification (NASBA-ELISA) was developed to fulfil the need for the efficient diagnosis of these ...the amplification of Aspergil- lus RNA by the ...
9
Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
... of nucleic acid sequence based amplification (NASBA) method is highly valuable for diagnosis of live parasite because there is no need for to use Thermo ...
7
Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... isothermal nucleic acid sequence-based amplification (NASBA) was developed to detect Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella ...
7
Nucleic Acid Sequence Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR
... Nucleic acid sequence-based amplification (NASBA) assays were developed for direct detection of Epstein- Barr virus (EBV) transcripts encoding EBV nuclear antigen 1 (EBNA1), latent ...
6
Analytical Performance and Clinical Utility of a Nucleic Acid Sequence Based Amplification Assay for Detection of Cytomegalovirus Infection
... A nucleic acid sequence-based amplification (NASBA) assay for qualitative detection of human cytomegalo- virus (CMV) pp67 mRNA was evaluated in a multicenter ...mRNA ...
6
Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification
... is based on microscopic detection of Plasmodium parasites in Giemsa-stained blood ...PCR based assays are sensitive and can be converted to a quantitative format if SYBR green or molecular probes ...tive ...
6
Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification
... assays based on Plasmodium antigen detection, but some of these tests perform poorly in cases of low parasitemia [7,9,10] or, in contrast, lead to a false positive diagnosis of malaria due to the persistence of ...
7
Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... using nucleic acid sequence-based amplification (NASBA) and detection with molecular beacon ...The amplification in the NASBA reaction involves the action of three enzymes: avian ...
10
RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples
... RNA amplification by nucleic acid sequence-based amplification (NASBA) for the detection of Chlamydia trachomatis infection was ...for amplification (10 23 IFU). In ...
7
Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... To confirm the presence of amplifiable DNA and to assess the effectiveness of the DNase treatments, the NA extracts were assayed by real-time PCR for the -globin gene (forward primer, 5⬘-CAG GTA CGG CTG TCA TCA CTT ...
6
Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... the amplification was 90 minutes (Blais et ...bead- based detection and electrochemiluminescent (ECL) detection and fluorescent correlation spectroscopy (Sergentet et ...
16
Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses
... rapidly detect WN virus in human clinical specimens is also significant, given the nonspecificity of the IgM ELISA and the time required to serologically confirm WN virus infection by the PRNT assay. The difficulty in ...
8
Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence Based Amplification
... (iii) Nucleic acid ...the nucleic acids for 10 min at room tempera- ...and nucleic acids were eluted by incubation at ...extracted nucleic acid was taken for ...
5
Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA
... (14). Based on results obtained with 216 genital swab specimens, the Basic Kit had sensitivities of ...transcription-mediated amplification tests for ...
7