OLIGONUCLEOTIDE PROBES
Species specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA directed oligonucleotide probes
... Oligonucleotide probes directed against rRNA targets have been used by several groups for the detection, species identification, and typing of isolates of several different organisms, in[r] ...
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Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities
... based oligonucleotide probes which are suitable for detection and quantification of Comamonas testosteroni in mixed cultures using PCR and/or fluorescent in situ hybridization ...
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Polymorphism of murine endogenous proviruses revealed by using virus class-specific oligonucleotide probes.
... 2 show clearly that the oligonucleotide probes can be used to detect the different families of endogenous nonecotropic proviruses, not only in HRS/J but also in other inbred strains, imp[r] ...
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Detection with synthetic oligonucleotide probes of nucleotide sequence variations in the genes encoding enterotoxins of Escherichia coli
... Oligonucleotide probes representing three regions of the genes encoding LT of human origin LTIh, the LT1 oligonucleotide probe from the coding region of the A subunit and the LT2 and LT3[r] ...
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Development of verotoxin 2 and verotoxin 2 variant (VT2v) specific oligonucleotide probes on the basis of the nucleotide sequence of the B cistron of VT2v from Escherichia coli E32511 and B2F1
... Development of Verotoxin 2- and Verotoxin 2 Variant VT2v-Specific Oligonucleotide Probes on the Basis of the Nucleotide Sequence of the B Cistron of VT2v from Escherichia coli E32511 and[r] ...
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Evaluation of oligonucleotide probes for identification of shiga like toxin producing Escherichia coli
... Four synthetic oligonucleotide probes representing different regions of the Shiga-like toxin I SLT-I structural genes and one oligonucleotide derived from the SLT-II gene of Escherichia [r] ...
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Development of novel fluorescent oligonucleotide probes for use in nucleic acid sequence analysis
... The solvents were removed in vacuo and the resulting oil dissolved in CH2Ch 200mL, washed with water 200mL, saturated sodium bicarbonate solution 200mL, brine 150mL and then dried Na2S04[r] ...
216
Species specific oligonucleotide probes for rRNA of Clostridium difficile and related species
... Given that nucleotide sequences in rRNA are known to reflect phylogeny, species-specific rRNA probes should be feasible if the sequences found in closely related species are different.. [r] ...
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M protein gene typing of Streptococcus pyogenes by nonradioactively labeled oligonucleotide probes
... When dot-blotted M-49 genomic DNA was hybridized to a probe designated emm49H whose sequence corresponded to nucleotide positions 164 to 193 i.e., spanning the codons of amino acids 5 to[r] ...
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Detection and identification of Candida species in experimentally infected tissue and human blood by rRNA specific fluorescent in situ hybridization
... Germany. Oligonucleotide O20 (5 9 -CCCC CTTTCCTAAACCAATCCGGA-3 9 ) is complementary to a sequence in the 18S rRNA of ...(12). Oligonucleotide Cpara (5 9 -CCCCCTTCTCTAAACCAATCCGGA-3 9 ) contains two ...
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Diagnostic Examination of Human Intestinal Spirochetosis by Fluorescent In Situ Hybridization forBrachyspira aalborgi, Brachyspira pilosicoli, and Other Species of the Genus Brachyspira(Serpulina)
... Human intestinal spirochetosis, characterized by end-on attachment of densely packed spirochetes to the epithelial surface of the large intestines as a fringe has been associated with the weakly beta-hemolytic ...
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Simian immunodeficiency virus displays complex patterns of RNA splicing.
... Colonies representing the PCR amplification products of the primer pairs PCRAR-PCR25 and PCRSTATPCR25 were initially screened by hybridization with oligonucleotide probes specific for sp[r] ...
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A Novel Class of Developmentally Regulated Noncoding RNAs in Leishmania
... end-labeled oligonucleotide probes for both the sense and antisense orientations were not equalized, so for this reason it was not possible to make a quantitative statement about the number of transcripts ...
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Oligonucleotide Gold Nanoparticle Networks for Detection of Cryptosporidium parvum Heat Shock Protein 70 mRNA
... The oligonucleotide probes were function- alized with thiol groups (carbon 6 S-S), hexaethylene glycol (18-atom), and poly(A) (15-nucleotide) spacers, followed by a 21-nucleotide sequence comple- mentary to ...
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Identification of Clinically Relevant Viridans Streptococci by an Oligonucleotide Array
... Hybridization procedures. Unless indicated otherwise, the hybridization pro- cedures were carried out at room temperature in a hybridization oven with a shaking speed of 60 rpm. All reagents except the buffers used for ...
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Glycoprotein hormone genes are expressed in clinically nonfunctioning pituitary adenomas
... specific oligonucleotide probes for the messenger (m)RNAs encoding growth hormone, prolactin, ACTH, and the glycoprotein hormone subunits, alpha, luteinizing hormone (LH)beta, follicle-stimulating hormone ...
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Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time resolved fluorescence based hybridization assay
... In this assay format, the PCR is run by standard procedures and the subsequent hybridization reaction is carried out in solution by using two oligonucleotide probes, one biotinylated and[r] ...
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Oligonucleotide Array Based Identification of Species in the Acinetobacter calcoaceticus A baumannii Complex in Isolates from Blood Cultures and Antimicrobial Susceptibility Testing of the Isolates
... of oligonucleotide probes. Four oligonucleotide probes (18- to 30- mers) based on the ITS sequences (Table 2) were designed to identify the four (genomic) species in the ...designed ...
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Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes
... 80 oligonucleotide probes were synthesized and printed onto the CombiMatrix CustomArray TM 4×2K microarray support to generate the prototype chips for the different influenza viruses and different sequences ...
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Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice
... create probes that can detect the presence or absence of indel sequences in a genomic DNA sample, the probes need to be complementary to a unique single copy ...where oligonucleotide probes ...
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