One-tube RT-PCR
One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3
... a one-tube reverse transcription-PCR (RT-PCR) assay, reference strains of DHAV-1 and DHAV-3 (isolated over a span of 60 years and covering many different countries) were successfully ...
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Development of a streamlined, selective enrichment culture, one tube (RT )PCR enzyme hybridization assay to detect bacterial fish pathogens in covertly infected farmed salmonids
... Therefore while nucleic acid probes are useful for the diagnosis of fish pathogens that are present in numbers sufficient to be found in situ or by blotting methods, for applications whe[r] ...
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Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one tube reverse transcription PCR assay
... each tube to yield final reaction volumes of 25 m ...pharmacy). RT-PCR was performed on a programmable thermocycler (MJR model PTC- 100-60; Fisher Scientific) as follows: 42 8 C for 30 min (cDNA ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... cost-effective RT-PCR assay based on SYBR green I has been developed for general screening of samples from suspected ...the PCR equipment used ...in one PCR run than in a ...
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Original Article Development and application of a rapid molecular method for detection of fusion genes in pediatric leukemia
... study, one pair of universal prim- ers and ten pairs of gene-specific chimeric primers were designed to develop one-step multiplex RT-PCR assay for simultaneous iden- tification of eight ...
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Fast Duplex One Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses
... specific one-step duplex reverse transcriptase PCR (RT-PCR) assay for the simultaneous and differential detection of West Nile (WNV) and Japanese encephalitis (JEV) ...duplex PCR using ...
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Standardization of one step single tube rt pcr for rapid detection of all circulating strains of dengue viruses
... ELISA, RT-PCR etc. RT- PCR assay is more sensitive than virus isolation, Virus isolation is although gold standard but time ...time PCR assay is most rapid, sensitive and specific but ...
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A SYBR Green RT PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
... are one of the main causes of foodborne gastroenteritis ...the one used in this assay [16]. Real-time RT-PCR assays are more and more devel- oped and has become the method of choice for the ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... in one test the detection of nine different microorganisms (enterovirus, influenza A and B viruses, respiratory syncytial virus [RSV], parainfluenzaviruses type 1 and type 3, adenovirus, Mycoplasma pneumoniae, and ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... conventional RT-PCR, the fluorogenic assay presents many advantages, the most important being a closed system in which the tube is never opened post amplification, reducing the possibility of ...
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... multiple PCR and mono RT-qPCR assays have been routinely applied in the well-equipped laboratory for the diagnosis of RSV, HRV and ...Mono RT-qPCR [11, 12, 14] cannot simultan- eously detect RSV, HRV ...
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Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
... on PCR technique offer a suitable alternative to conventional isolation ...The RT-PCR targeting the conserved regions of C-prM gene junction is widely employed for precise confirmation of an ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... The hnRT-PCR technique allows a sensitive, specific and fast diagnosis for rabies virus, even when samples are in a decomposed state. Additionally, the amplified products can be used in techniques such as ...
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Nützel, Friedrich (2005): Relative Quantifizierung vaskulogenese- und angiogeneseassoziierter Wachstumsfaktoren der frühen Embryonalentwicklung des Huhns (Gallus gallus domesticus) mittels der real-time RT-PCR. Dissertation, LMU München: Tierärztliche Fakultät
... vergleichenden Studie der ersten vier Methoden (Gruffat et al. 1996) zeigte sich die Überlegenheit der Methode nach Chirgwin et al. hinsichtlich Ausbeute und Integrität der RNA. Neben diesen beiden Parametern muss die ...
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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
... detection, one primer– probe combination was also selected from combinations proposed by the PRIMER EXPRESS software (Applied Biosystems, USA) according to the manufacturer’s instruc- ...
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Specific detection of H5N1 avian influenza A virus in field specimens by a one step RT PCR assay
... cater for use in the field in regions where real-time PCR platform and equipments may not be available. Clearly, the results would be further strengthened with the inclu- sion of more known H5N1 influenza in ...
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Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... nested PCR was done with a touchdown amplification profile adapted according to Sachadyn et ...round, RT at 53°C for 30 min; PCR with 20 cycles of 95°C for 1 min, 65°C for 1 min, and 72°C for 1 min; ...
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Evidence that the intra amoebal Legionella drancourtii acquired a sterol reductase gene from eukaryotes
... Although the role of the sterol delta-7 reductase is not clearly identified in prokaryotes, there is evidence that some intracellular bacteria can interfere with the host cho- lesterol metabolism. C. burnetii upregulates ...
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Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription PCR with an Armored RNA Internal Control
... an RT-PCR assay in a nonnested format for the detection of EV RNA in clinical specimens in which 2,500 copies of armored IC RNA mimicking the EV target ...
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... and one was incorporated into the probe ...real-time RT-PCR assay, since this strain is representative of H5 clade 3 viruses, which have not been detected since 1997, and can easily be distinguished ...
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