PARP 1
Original Article Effect of adriamycin on BRCA1 and PARP-1 expression in MCF-7 breast cancer cells
... with 1 × cell lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% Triton, ...pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na 3 VO 4 , and 1 μg/ ...
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Original Article Function and mechanism of combined PARP-1 and BRCA genes in regulating the radiosensitivity of breast cancer cells
... that PARP-1 inhibitor 3-AB can increase the radiosensitivity of BRCA mutated (MDA- MB-231) and BRCA non-mutated breast can- cer cells (MDA-MB-436), with marked increase of radiation sensitivity in ...
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Olaparib based photo-affinity probes for PARP-1 detection in living cells
... the UV irradiation step was omitted yielding no signal, showing that the observed labeling is UV-dependent rather than circumstantial activation. As an additional control, the gel used for in-gel fluorescence readout was ...
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PARP-1 controls NK cell recruitment to the site of viral infection
... of PARP-1? Macrophages play a crucial role in the control of invading pathogens through the production of reactive oxygen species (ROS) or reactive nitrogen species (RNS) (34), which can damage cellular DNA ...
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A PET imaging agent for evaluating PARP 1 expression in ovarian cancer
... CRISPR/Cas9 deletion of PARP1 in ovarian cancer cells. Using CRISPR/Cas9 gene editing, we mediated the deletion of PARP1 in 2 ovarian cancer cell lines, one with a BRCA1 mutation and another with BRCA1 promoter ...
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Synthesis and evaluation of a radioiodinated tracer with specificity for poly(ADP-ribose) polymerase-1 (PARP-1) in vivo
... PARPi 1 in the pursuit of a novel PARP-1 SPECT imaging ...of 1 to the PARP-1 active site can tolerate structural modifications in the cyclopropane bearing ...of 1 with ...
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Original Article Potential role of HGF-PARP-1 signaling in invasion of ovarian cancer cells
... Total RNA were isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the man- ufacturer’s protocol. The purified RNA was suspended in diethyl pyrocarbonate (DEPC)- treated water. Ten microliters of total ...
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Partial protection against collagen antibody induced arthritis in PARP 1 deficient mice
... integrity. PARP-1 detects DNA breakage generated by several genotoxic agents and synthesizes and transfers ADP ribose units (poly(ADPribosyl)ation activity) into acceptor proteins involved in the ...
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PARP-1 cleavage fragments: signatures of cell-death proteases in neurodegeneration
... caspase-3, PARP-1 is also cleaved by these 3 pro- teases resulting in different fragmentation patterns ...though PARP-1 is a direct substrate for Gra-A it can only cleave intracellular ...
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The Inhibition and Treatment of Breast Cancer with Poly (ADP-ribose) Polymerase (PARP-1) Inhibitors
... The in vitro experiments were followed by in vivo examination of BRCA1+/+ and BRCA1-/- mammary tumors exposed to 5 days of high dose AG14361 treatment. Inhibition of mammary tumor growth was observed two days after the ...
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and PARP-1 inhibitor DPQ on glioma by a Ca 2+ -Mg 2+ -endonuclease pathway
... We found that after 24-48 h treatment, 25-100 μM DPQ induced C6 cell death in a dose-depen- dent manner (Figure 4A). ATA also had good protective function against DPQ-induced C6 cell death (Figure 4B), which means CME ...
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PARP-1 activity is required for the reconsolidation and extinction of contextual fear memory
... that PARP-1 ac- tivity is necessary to induce hippocampal expression of the CREB-target gene c-fos after training in an object recogni- tion task ...that PARP-1 inhib- ition in the hippocampus ...
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Investigation of PARP-1, PARP-2, and PARG interactomes by affinity-purification mass spectrometry
... of PARP-1, PARP-2 and PARG in the regulation of RNA splicing is corroborated by many stud- ...endogenous PARP-1 and PARG regulate pADPr binding to hnRNPs, subsequently altering the ...
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Crosstalk between Autophagy and Apoptosis Modulates T-2 Toxin-Induced Toxicity in Liver Cells
... After treatment, the L02 cells were lysed with RIPA buffer containing complete Mini protease inhibitor cocktail (Beyotime, Nantong, China). After centrifugation, the supernatant was collected and stored at -80°C. Protein ...
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Zinc Inhibits Astrocyte Glutamate Uptake by Activation of Poly(ADP-ribose) Polymerase-1
... merase 1 (PARP-1), thus impairing en- ergy metabolism and glutamate uptake in these ...cells. PARP activity is shared by a family of enzymes, of which PARP-1 is the most abundant ...
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Poly(ADP-Ribose) Polymerase 1 Is Not Strictly Required for Infection of Murine Cells by Retroviruses
... The HIV-sinPPT and HIV-EGFP⌬E vectors are both VSV- G-pseudotyped derivatives of the NL4-3 isolate, but they differ in their composition. The HIV-EGFP⌬E virus was constructed from the NL4-3 proviral clone by removing the ...
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Poly(ADP ribose) polymerase as a novel regulator of 17β estradiol induced cell growth through a control of the estrogen receptor/IGF 1 receptor/PDZK1 axis
... the PARP family of proteins, has initially been described as a DNA repair enzyme playing primarily as a regulatory protein controlling traffic of DNA repair proteins during base excision repair [5, ...of ...
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Simian Virus 40 Infection Triggers a Balanced Network That Includes Apoptotic, Survival, and Stress Pathways
... signaling. PARP-1 is activated by automodification by the addition of poly(ADP-ribose) ...activated PARP-1 may be sig- nificantly larger than nonactive PARP-1 and readily distin- ...
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Disrupted ADP ribose metabolism with nuclear Poly (ADP ribose) accumulation leads to different cell death pathways in presence of hydrogen peroxide in procyclic Trypanosoma brucei
... human PARP crystal structures. Sequence alignment included PARP regula- tory (REG) and ADP-ribosyl transferase (CAT) domains as they are conserved in hPARP1-3 and in TbPARP ...many PARP inhibitors, ...
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Myocardial Postischemic Injury Is Reduced by PolyADPribose Polymerase-1 Gene Disruption
... or PARP-1 -/- isolated perfused mouse hearts to I/R and monitored PARP activity in rat heart slices with the PARIS assay ...the PARP inhibitor benzamide (1 mM). PARP activity ...
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