PCR-amplification
Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... influence amplification of the target gene and act as PCR ...investigate PCR amplification differences between these species of goatfishes and to optimize protocols for PCR ...
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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
... of PCR- amplified libraries of cloned 18S rRNA genes, suggest that this single species is the predominant alga in the Nymph Creek mat at temperatures above ...
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Rapid Determination of Quinolone Resistance in Acinetobacter spp
... that PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) and base composition analysis of PCR amplification products can quickly and accurately identify quinolone resistance ...
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Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay
... and PCR typing are laborious and ...on PCR amplification of the target region of the ent genes with degenerate primers, followed by characterization of the PCR products by microchip ...
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Optimization and validation of multi coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)
... (PCR) amplification of the polymorphic genes encoding the merozoite surface proteins 1 and 2 (MSP1 and MSP2) and the glutamate-rich protein (GLURP) ...genes, amplification generates DNA frag- ments ...
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Comparison of Different DNA Fingerprinting Techniques for Molecular Typing of Bartonella henselaeIsolates
... PCR amplification and restriction analysis of the spacer region between the 16S and 23S ...the amplification of the spacer region separating the 16S and 23S ...The amplification was performed ...
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Simple PCR Based DNA Microarray System To Identify Human Pathogenic Fungi in Skin
... of PCR amplification and DNA microarray assay (designated PCR-DM), in which PCR-amplified DNA from the internal transcribed spacer region of the rRNA gene was hybridized to a DNA microarray ...
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Are Molecular Tools Solving the Challenges Posed by Detection of Plant Pathogenic Bacteria and Viruses?
... on PCR or RT-PCR now routinely applied ...of PCR amplification and very especially on real-time PCR are leading to high throughput, faster and more accurate detection methods for the ...
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Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens
... Preparation of samples for PCR amplification. Genomic DNAs were ex- tracted from all strains by the guanidium thiocyanate method (20). The extracted DNAs were dissolved in water, and solutions containing 25 ...
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A METHODOLOGICAL COMPARISON AMONG DNA SOURCE TYPES FOR MOOSE GENOTYPING
... all PCR to bind potential inhibi- tors and improve amplification specificity (Kreader ...to PCR was initially implemented to increase ampli- fication success using DNA extracted from fecal ...to ...
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Rapid determination of clonality by detection of two closely linked X chromosome exonic polymorphisms using allele specific PCR
... (13), PCR-ASOH (14), and PCR amplification of specific allele (PASA) (15) into a simpler as- say (allele–specific PCR) for genotype screening and clonality determination based on G6PD and p55 ...
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Molecular Differentiation of SevenMalassezia Species
... PCR amplification of the ITS region with primers ITS 1 and ITS 4 (Table 2) readily distinguished ...five PCR-REA types, desig- nated types C, C⬘, D, D⬘, and E ...unique PCR-REA type, C⬘, ...
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Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays
... IAC amplification is ...the PCR has occurred and the test for that sample is not ...a PCR in which two reactions with different kinetics proceed simulta- ...approach, PCR amplification ...
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Specificities and Functions of the recA and pps1 Intein Genes of Mycobacterium tuberculosis and Application for Diagnosis of Tuberculosis
... through PCR amplification of mycobacterium-specific genes, designed to shorten diagnostic delay, demon- strated reliability and high ...the PCR amplification of recA and pps1 intein genes as a ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... volume of 25 l with 20 ng template DNA per reaction mixture, under the following PCR conditions: denaturation at 95°C for 10 min followed by 40 cycles at 95°C for 20 s, 62°C for 40 s, and 72°C for 30 s. Melt ...
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Detection of group B and C rotaviruses by polymerase chain reaction
... Ethanol precipitation of the remaining 45 gil of the eluate before PCR/C amplification, instead of using only 5 gil of the eluate directly for amplification, did not alter the detection [r] ...
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Internal amplification control of PCR for the Glu1-Dx5 allele in wheat.
... internal amplification control is a DNA sequence that is amplified in all samples and co-amplified in the same PCR of the target ...internal amplification control, the control sign will be produced ...
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Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets
... amplifying RNA targets (SMART), designed to minimize current limitations in the various amplification schemes (Fig. 1) (22). The SMART (modified NASBA) does not amplify the RNA molecule itself but rather the ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the rapid diagnosis and identification of dengue virus serotypes and have ...
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PCR based study of conserved and variable DNA sequences of Tritrichomonas foetus isolates from Saskatchewan, Canada
... Single-band PCR products were verified by Southern blotting and hybridization with labeled oligonucleotide ...probes. PCR products were transferred from agarose gels to Nytran (Schleicher and Schuell) by ...
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