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PCR and RT-PCR

Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses

Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses

... conventional PCR using the type-specific T7 polymerase promoter-labeled forward primer and type-specific reverse ...for RT-qPCR and 1e2 to 1e6 copies/reaction for RT-dPCR) were amplified using the ...

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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus

Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus

... I-based RT-PCR and the mul- tiplex PCR differs depending on the template ...green RT- PCR displayed equal or higher sensitivity than the probe-based multiplex ...green ...

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Development of a Microplate Lectin Capture RT PCR (MLC RT PCR) for the Detection of Avian Infectious Bronchitis Virus

Development of a Microplate Lectin Capture RT PCR (MLC RT PCR) for the Detection of Avian Infectious Bronchitis Virus

... Based on the binding capacity of Con A with mannose moieties of viral glycoproteins, this lectin has been used for the separation of RNA viruses, as togaviruses, influ- enzavirus, parainfluenzavirus, and rhabdoviruses ...

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Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR

Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR

... positive RT-PCR marker expression and prognostic indicators were analysed using Fisher's exact ...quantitative RT-PCR multi- marker assay of blood with tumour specific markers reported a ...

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Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR

Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR

... TaqMan RT-PCR detected DOBV RNA in 7 of 474 rodent tissue ...nested PCR yielded a 389-bp band for 2 of 474 rodent tissue ...TaqMan RT-PCR (Ta- ble ...nested PCR by use of the ...

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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis

Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis

... the RT-PCR assay. RT-PCR failed to identify some specimens ...corresponding RT- PCR primers and ...Overall, RT-PCR was relatively easy to perform and more sensitive ...

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RT-PCR Detection of Coxsackievirus B3: A Viral Myocarditis

RT-PCR Detection of Coxsackievirus B3: A Viral Myocarditis

... the RT- PCR ...the RT-PCR assay. Sensitivity of the RT-PCR assays was 1 to 10 -2 TCID50/100 µl in various experiments ...the RT- ...and RT-PCR in order to ...

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A novel method for the normalization of microRNA RT-PCR data

A novel method for the normalization of microRNA RT-PCR data

... with RT-PCR that remains as yet unaddressed by current normalization methods is the systematic bias present within the ...the PCR magnifica- tion at each cycle is an exact doubling of the expres- ...

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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype

Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype

... digital RT-PCR method for rapid detection of H9 subtype influenza was es- tablished by comparing the two methods of digital RT-PCR and real-time quantitative ...

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Development of elisa and rt-pcr assays for rapid and sensitive detection of imperata yellow mottle virus infecting maize in burkina faso

Development of elisa and rt-pcr assays for rapid and sensitive detection of imperata yellow mottle virus infecting maize in burkina faso

... test, RT-PCR was found more sensitive in detecting local IYMV ...sensitive RT-PCR are a practical approach for future epidemiological studies of IYMV and acquiring information about the viral ...

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Nützel, Friedrich
  

(2005):


	Relative Quantifizierung vaskulogenese- und angiogeneseassoziierter Wachstumsfaktoren der frühen Embryonalentwicklung des Huhns (Gallus gallus domesticus) mittels der real-time RT-PCR.


Dissertation, LMU München: Tierärzt

Nützel, Friedrich (2005): Relative Quantifizierung vaskulogenese- und angiogeneseassoziierter Wachstumsfaktoren der frühen Embryonalentwicklung des Huhns (Gallus gallus domesticus) mittels der real-time RT-PCR. Dissertation, LMU München: Tierärztliche Fakultät

... vergleichenden Studie der ersten vier Methoden (Gruffat et al. 1996) zeigte sich die Überlegenheit der Methode nach Chirgwin et al. hinsichtlich Ausbeute und Integrität der RNA. Neben diesen beiden Parametern muss die ...

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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples

Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples

... Background: The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) ...

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Evidence that the intra amoebal Legionella drancourtii acquired a sterol reductase gene from eukaryotes

Evidence that the intra amoebal Legionella drancourtii acquired a sterol reductase gene from eukaryotes

... Although the role of the sterol delta-7 reductase is not clearly identified in prokaryotes, there is evidence that some intracellular bacteria can interfere with the host cho- lesterol metabolism. C. burnetii upregulates ...

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Gene Expression Induced by Copper Stress in the Diatom Thalassiosira pseudonana

Gene Expression Induced by Copper Stress in the Diatom Thalassiosira pseudonana

... posttranscriptional and posttranslational) regulation of antioxi- dant enzymes has been described for a number of organisms (8, 44), and the level of regulation of antioxidant genes in T. pseud- onana is not known at ...

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A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids

A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids

... resulting RT-PCR product was cloned between the EcoRI and StuI sites in the multiple cloning site of the pFastBac1 baculovirus expression vector and named pclone ...7-kb PCR product that was cut with ...

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Evaluation of a Nested Reverse Transcription PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections

Evaluation of a Nested Reverse Transcription PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections

... (n RT-PCR-N) of the bovine respiratory syncytial virus (BRSV) has been developed and optimized for the detection of BRSV in bronchoalveolar lavage fluid cells of ...n RT-PCR-N showed the ...

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Phylogenic analysis of the M genes of influenza viruses isolated from shorebirds

Phylogenic analysis of the M genes of influenza viruses isolated from shorebirds

... Tracheal and cloacal swabs were collected from a total of 195 juvenile shorebirds in the area in 2012 in Eastern Hokkaido, Japan. Viruses showing hemagglutination activity were successfully recovered from 28 bird ...

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Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA

Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA

... real-time PCR and, in parallel, the corresponding single-step real-time PCR ...real-time PCR, are shown in Table 3. For all three assays, seminested real-time (RT-)PCR was clearly ...

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Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay

Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay

... usual PCR, after that, ELISA is performed to receive desired ...time. PCR-ELISA does not require using dangerous and carcinogenic material that have used in many of tech- niques, including of methylene blue ...

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Original Article Development and application of a rapid molecular method for detection of fusion genes in pediatric leukemia

Original Article Development and application of a rapid molecular method for detection of fusion genes in pediatric leukemia

... fluorescent PCR products onto bead-probe complexes in solution, and fluorescence analy- sis on a flow ...multiplex RT-PCR method combined with capillary elec- trophoresis as a multiplex gene ...

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