PCR / RAPD
oa Durban Museum Novitates - Genetic variation in Arvicanthis (Rodentia: Muridae) from Sudan: an analysis based on cytosystematic and PCR-RAPD data
... Genetic variation in Arvicanthis (Rodentia: Muridae) from Sudan: an analysis based on cytosystematic and PCR- RAPD data.. The genus Arvicanthis Lesson, 1842 (Rodentia; Murida[r] ...
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Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran
... Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various ...
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RAPD-PCR characterization of insecticide resistant and susceptible Anopheles species
... nomic DNA isolated from the two species were used for characterisation of the insecticide resistance and susceptibility using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD- PCR). 15 ...
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RAPD-PCR characterization of Bacillus thuringiensis soil isolates from Mizoram with insecticidal activity against Culex tritaeniorhynchus
... higher larvicidal activity than reference strain against both A. aegypti and C. quinquefasciatus. The variation within the type of cry genes was observed in the chosen Bt isolates. There is no relationship between the ...
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A COMPARATIVE STUDY BETWEEN A NUMERICAL AND RAPD-PCR METHODS FOR THE IDENTIFICATION OF SOME STREPTOMYCETE STRAINS
... On the other hand, a high difference (38.8%) between the two analyses was found when used for the identification of W86 (R/RF/C-/SM) and BB24 (R/RF/C- /SM) Streptomyces strains of group 4 (Table 8). Data also showed ...
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Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
... radiations produce a higher rate of single-stranded breaks (SSBS) in DNA than double-stranded breaks (DSBS), per unit dose, compared to high-LET radiations (10, 11). Gamma irradiation produces oxygen radicals generated ...
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Morphological and molecular studies of six Junonia species of butterflies using RAPD-PCR technique
... RAPD random primers. Primers were ob- tained from Biosciences, India. 10 µl of reac- tion mixture contained 1 µl of 10X PCR buffer, 1 µl MgCl (25 mM), 0.2 µl dNTPs (2 mM), 0.3 µl of Taq polymerase (3 U/µl), ...
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Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques
... in RAPD profiles of Zea mays seeds treated with Verbascum speciosum extract included variation in band intensity, loss of bands and appearance of new bands compared with ...
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SENSITIVITY AND SPECIFICITY OF CONVENTIONAL CULTURE, LIGHT MICROSCOPY, SEROLOGICAL AND MOLECULAR METHODS FOR IDENTIFYING Mycobacterium tuberculosis COMPLEX
... IS6110-targed PCR, 30 and 90% for ELISA, ...IS6110-targed PCR was relatively more useful in TB diagnosis followed by ...identification. RAPD-PCR finding showed some molecular DNA markers that ...
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Different Molecular Methods to Determine Genetic Diversity in Pseudomonas aeruginosa Clinical Isolates
... that RAPD-PCR markers are powerful and effective techniques in identify and initial screening of samples, examining the differences among species and identify at the level of strain as well as compared with ...
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DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan
... of RAPD-PCR. Sixty RAPD makers were selected from BioNeer kits and applied against the 15 DNA ...9 RAPD primers were chosen out of 60 for further ...nine RAPD primers generated 51 ...
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Efficiency of RAPD in assessing genetic variation in commercially viable local varieties of blueberry (Vaccinium)
... of RAPD-PCR, it is possible to differentiate between different varieties of Vac- cinium grown in Chile in order to then optimize blueberry production in terms of time and re- ...
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Molecular Characterization of Invasive and Noninvasive Campylobacter jejuni and Campylobacter coli Isolates
... this RAPD marker ...a PCR using specific primers that ampli- fied a fragment of the iam ...This PCR appears to be useful for the identification of invasive strains, since it accurately classified 82% ...
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Outbreak of Staphylococcus schleiferiWound Infections: Strain Characterization by Randomly Amplified Polymorphic DNA Analysis, PCR Ribotyping, Conventional Ribotyping, and Pulsed Field Gel Electrophoresis
... and PCR-mediated ribotyping, and pulsed-field gel electrophoresis [PFGE] of DNA macro restriction fragments), appeared to be limited both among the clinical isolates and among several control strains obtained from ...
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Application of Randomly Amplified Polymorphic DNA (RAPD) Technique to Estimate Genetic Distance among Some Methicillin Resistant Staphylococcus aureus Isolated from Different Iraqi Hospitals Alyaa Mohammed H.1* Maysaa Adil Ali2 Mohammed Saad Alnasari3 Wedean Al-Hadban3
... simple PCR based method, is used widely for epidemiological studies. RAPD primers can efficiently scan whole chromosomes for the presence of short inverted DNA sequences and amplifies the inverted ...
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Madurella mycetomatis Strains from Mycetoma Lesions in Sudanese Patients Are Clonal
... high-throughput RAPD and newly developed PCR-RFLP tests were applied to detect possible genetic vari- ation among different clinical ...The RAPD results were frustrating: out of 20 primer species ...
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Analysis of Antibiotic Susceptibility Profile and RAPD Typing of Listeria Monocytogenes Isolates
... Genotyping by RAPD-PCR showed that the chicken L. monocytogenes isolates were diverse and heterogeneous. Since the isolates were from different areas and various sampling times, such heterogeneity might be ...
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Molecular diversity of brinjal (Solanum melongena L. and S. aethiopicum L.) genotypes revealed by SSR markers
... The Polymerase Chain Reaction (PCR) based molecular markers such as RAPD, SSR and ISSR have successfully been utilized to assess the genetic diversity among the genotypes of several crop plants. Out of ...
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Antibacterial Activity of Halodule Uninervis Leaves Extracts Against Some Bacterial Pathogens Strains
... Polymorphism was exhibited in twelve of the preselected random primers (RAPD) obtained from the DNAs of seven samples of PC: Pseudomonas aeruginosa Control, PT: Pseudomonas aeruginosa Treatment. Study P. ...
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Assessment of diversity using RAPD and ISSR markers in Sorghum varieties across Gujarat, India
... Spectrophotometer, Thermo Scientific). The extracted DNA samples were then used for RAPD and ISSR analysis. The details of RAPD and ISSR marker used in the study were presented in Table 3. The Polymerase ...
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