PCR restriction enzyme analysis
Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR Restriction Enzyme Analysis of hsp65
... the restriction enzyme; that tolerance was different from the position tolerances of ...using restriction enzymes that generated DNA fragments in the 800- to 100-bp range that were separated on ...
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Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... avium PCR-restriction enzyme analysis (PRA) variants were identified, differing from the ...by PCR with DT1-DT6 and IS1245 primers, nucleic acid hybridization with the Accu- Probe ...
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PCR Restriction Enzyme Analysis for Detection ofCandida DNA in Blood from Febrile Patients with Hematological Malignancies
... the PCR-REA with respect to the development of disseminated candidiasis was quite high ...the PCR-negative patients were taken off amphotericin B therapy after 2 to 3 days (because they were afebrile and ...
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Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR restriction enzyme analysis, 16S rRNA gene sequencing, and DT1 DT6 PCR
... DT1 PCR, which did not fail to detect any of the 19 isolates studied here (considered 100% detection), ...DT1-DT6 PCR (they were DT6 negative and DT1 positive) and tentatively identified as ...DT1 ...
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Differentiating Taenia solium andTaenia saginata Infections by Simple Hematoxylin Eosin Staining and PCR Restriction Enzyme Analysis
... of PCR and restric- tion enzyme analysis (REA), which can be used to identify these tapeworms by using DNA from proglottids whether they are gravid or not, or from ...The PCR-REA we describe ...
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Identification of various medically important Candida species in clinical specimens by PCR restriction enzyme analysis
... 378C for 15 min. After centrifugation (10,000 3 g for 30 min), the pellets were resuspended in 0.5 ml of 1 M sorbitol–50 mM phosphate buffer (pH 7.5) con- taining 2% b-mercaptoethanol and 2 mg of yeast lytic ...
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Comparison of Restriction Enzyme Analysis, Arbitrarily Primed PCR, and Protein Profile Analysis Typing for Epidemiologic Investigation of an Ongoing Clostridium difficileOutbreak
... [PFGE], PCR, arbitrarily primed PCR [AP-PCR], restriction enzyme analysis [REA], plasmid profile analysis, and ribotyping) and nongenomic typing methods (pro- tein profile ...
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An Outbreak of Keratitis Caused by Mycobacterium immunogenum
... by PCR restriction enzyme analysis of the hsp65 gene and PCR restriction enzyme analysis of the 16S-23S rRNA internal transcribed spacer (ITS) was isolated from ...
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Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp capripneumoniae by PCR and restriction enzyme analysis
... a PCR system by which a segment of the 16S rRNA gene from all mycoplasmas of the ...The PCR product is then analyzed by restriction enzyme cleavage for the identification of ...by ...
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Molecular Analysis of Mycobacterium kansasii Isolates from the United States
... by PCR restriction enzyme analysis (PRA) of the 441-bp Telenti fragment of the hsp-65 gene and pulsed-field gel electrophoresis (PFGE) of genomic DNA with the restriction endonucleases ...
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Clinical Application of PCR Restriction Enzyme Pattern Analysis for Rapid Identification of Aerobic Actinomycete Isolates
... of PCR-restriction enzyme pattern analysis (PRA) for routine identification of aerobic actinomycete clinical isolates were evaluated for 299 cultures submitted to the Mycobacteria/Nocardia ...
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Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... this enzyme has polymorphic repeat regions in its 3′ end sequence, it is thought that RFLP analysis of the coa gene could be useful for typing of ...distinct PCR amplification of this region and lead ...
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Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome
... on restriction enzyme digestion of a large amount of high quality (high molecular weight) genomic ...further analysis, such as gel electrophoresis or Sanger sequencing, is required to confirm insert ...
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Use of PCR Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene Based Identification of Nocardia Species
... Analysis of the clinical isolates indicated that (i) species discrimination was better with hsp65 than with 16S rRNA (larger distances between the closest species pairs) and (ii) combined analysis of the ...
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Potential Value of Major Antigenic Protein 2 for Serological Diagnosis of Heartwater and Related Ehrlichial Infections
... by PCR cloning methods, and restriction enzyme anal- ysis was performed and compared with that for the previously reported map2 gene of the Crystal Springs ...strain. Restriction map ...
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Rapid detection and characterization of foot and mouth disease virus by restriction enzyme and nucleotide sequence analysis of PCR products
... with PCR was used for the detection of foot-and-mouth disease virus serotypes A, C, and O in organ extracts from experimentally infected ...isolates restriction enzyme cleavage and nucleotide ...
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Identification of murine helicobacters by PCR and restriction enzyme analyses
... the PCR assay developed in this study, which detects and identifies multiple Helicobacter species, an ...species-specific PCR assay was recently described by Battles and colleagues ...species-specific ...
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Detection and identification of mycobacteria in formalin fixed, paraffin embedded tissues by nested PCR and restriction enzyme analysis
... Preparation of PET specimens. By following the protocol of Wright and Manos (24), PET specimens were used as the test material for the diagnostic PCR assay. Briefly, two sections from PET blocks (10 m m thick) ...
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Restriction enzyme digestion analysis of PCR amplified DNA of Blastocystis hominis isolates
... the PCR product of about 1,770 bp with all the 7 Blastocystis isolates ...The restriction fragment length polymorphism (RFLP) patterns yielded by 13 out of 25 restriction endonucleases showed that ...
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Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
... polymorphism analysis (PCR-RFLP) PCR was followed by PCR-RFLP ...the PCR product, 4 μl of PCR product was digested with 10U of the restriction enzyme; ApaI ...
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