PCR-restriction fragment length polymorphism
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... equipment, PCR-restriction fragment length polymorphism analysis (PRA) (26) of the hsp65 gene present in all mycobacteria (9, 20, 21) offers an easy, rapid, and inexpensive procedure to ...
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Diversity of Staphylococcus Species Strains Based on Partial kat (Catalase) Gene Sequences and Design of a PCR Restriction Fragment Length Polymorphism Assay for Identification and Differentiation of Coagulase Positive Species (S aureus, S delphini, S hyicus, S intermedius, S pseudintermedius, and S schleiferi subsp coagulans)
... degenerate PCR primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal ...nucleotide polymorphism revealed in this gene could be exploited ...
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Phylogenetic Analysis and PCR Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences
... 517-bp fragment of the groEL gene was developed and used for PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of 68 strains representing 11 Campylobacter ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... A PCR strategy selectively amplifies genomic DNA from molds belonging to the genera Absidia, Mucor, Rhizopus, and Rhizomucor, excluding human DNA and DNA from other filamentous fungi and ...this ...
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Can the fliC PCR Restriction Fragment Length Polymorphism Technique Replace Classic Serotyping Methods for Characterizing the H Antigen of Enterotoxigenic Escherichia coli Strains?
... fliC PCR-restriction fragment length polymorphism (RFLP) to investigate whether this technique would be better than classic serotyping for the characterization of the H antigen in ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species ...
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Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... appropriate restriction buffer (10⫻), and 15 l of autoclaved distilled ...one, restriction fragments were electrophoresed on a 3% agarose gel at 120 V for 2 h, and in the other, restriction ...
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... by PCR with forward primer SSU- bact-27f (5⬘-AGA GTT TGA TCM TGG CTC AG-3⬘) and reverse primer SSU-bact-907r (5 ⬘ -CCG TCA ATT CMT TTR AGT TT-3 ⬘ ), which have been described previously ...
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Molecular typing of Borrelia burgdorferi sensu lato by PCR restriction fragment length polymorphism analysis
... The molecular analysis of individual isolates of B. burgdorferi and correlation of particular bacterial characteristics with spe- cific disease manifestations are limited to culturable isolates of B. burgdorferi sensu ...
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Simple, Low Cost Molecular Assays for TR34/L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole Resistant Aspergillus fumigatus Isolates
... of PCR products was run on 2% agarose ...a PCR product purification kit (Qiagen) according to instructions sup- plied with the ...generate PCR-restriction fragment length ...
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Clostridium difficile Genotyping Based on slpA Variable Region in S Layer Gene Sequence: an Alternative to Serotyping
... by PCR from serogroup reference strains and various clinical isolates chosen ...after restriction enzyme digestion and DNA ...that PCR-restriction fragment length ...
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Mycobacterium bovis subsp caprae Caused One Third of Human M bovis Associated Tuberculosis Cases Reported in Germany between 1999 and 2001
... The prevalence of the Mycobacterium bovis subsp. caprae and M. bovis subsp. bovis among German tubercu- losis cases caused by the bovine tubercle bacillus from 1999 to 2001 was determined. Isolates from 166 patients ...
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Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... amplified fragment length polymorphism (5, 15), flagella gene PCR-restriction fragment length polymorphism (PCR-RFLP) (1, 4, 23, 24, 26), ribotyping (6, 25, ...
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Madurella mycetomatis Strains from Mycetoma Lesions in Sudanese Patients Are Clonal
... species-specific PCR restriction fragment length polymorphism (RFLP) tests were ...seven PCR RFLP tests were successfully performed on the 38 different ...
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Identification of Mycobacterial Species by PCR Sequencing of Quinolone Resistance Determining Regions of DNA Gyrase Genes
... require PCR sequencing of long DNA ...using PCR- restriction fragment length polymorphism, and some laborato- ries found it simple and inexpensive (10, 12, 17, 19, ...of ...
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Genotyping, Drug Susceptibility and Prevalence Survey of Trichomonas vaginalis among Women Attending Gynecology Clinics in Hamadan, Western Iran, in 2014-2015
... and PCR-restriction fragment length polymorphism (RFLP) ...and PCR-RFLP analy- sis identified three actin type; A (9, 56%), I (6, 38%) and E (1, ...
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The involvement of Kras gene 3'-UTR polymorphisms in risk of cancer and influence on patient response to anti-EGFR therapy in metastatic colorectal cancer: a meta-analysis
... allele-specific PCR; PCR, polymerase chain reaction; PCR-RFLP; PCR–restriction fragment length polymorphism; BEL, Belgium Ovarian Cancer study; BWh, Brigham Women’s ...
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A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
... CHROMagar, PCR-restriction fragment length polymorphism (PCR-RFLP), and PCR-fragment size polymorphism (PCR-FSP) assays in the identification of ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... including PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism (RFLP), and amplified fragment length polymorphism (AFLP) ...
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Comparative Typing of Campylobacter jejuni by Heat Stable Serotyping and PCR Based Restriction Fragment Length Polymorphism Analysis
... and PCR-based restriction fragment length polymorphism (RFLP) analysis of the lipooligosaccharide (LOS) biosynthesis genes of ...DNA fragment of the LOS gene cluster was ...
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