PCR–restriction fragment length polymorphism
Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... 2000 PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the ...
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus ...
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Sensitive PCR Restriction Fragment Length Polymorphism Assay for Detection and Genotyping of Giardia duodenalis in Human Feces
... heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia duodenalis on the basis of polymorphism in the triose phosphate ...
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Recognition of a Nocardia transvalensis complex by resistance to aminoglycosides, including amikacin, and PCR restriction fragment length polymorphism analysis
... 2. PCR-restriction fragment length polymorphism (RFLP) analysis of a 439-bp segment of the 65-kDa heat shock protein gene with XhoI and HinfI produced identical patterns for 53 (91%) ...
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Development of a PCR Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin Producing Escherichia coli
... a PCR-restriction fragment length polymorphism (RFLP) assay for the epidemiological analysis of Shiga toxin- producing Escherichia coli (STEC) on the basis of the diversity of region ...
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Comparison of PCR Restriction Fragment Length Polymorphism Analysis and PCR Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants
... utilizing PCR-restriction fragment length polymorphism (RFLP) in the Helicobacter pylori genes is widely used to differentiate ...specific restriction site can be ...the ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... A PCR strategy selectively amplifies genomic DNA from molds belonging to the genera Absidia, Mucor, Rhizopus, and Rhizomucor, excluding human DNA and DNA from other filamentous fungi and ...
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Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... of restriction fragments as deduced by sequence analysis and allowed identification of mycobacteria whose PRA pat- terns were unequivocally identified by fragments shorter than 60 ...yielded fragment ...
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Molecular typing of Borrelia burgdorferi sensu lato by PCR restriction fragment length polymorphism analysis
... The molecular analysis of individual isolates of B. burgdorferi and correlation of particular bacterial characteristics with spe- cific disease manifestations are limited to culturable isolates of B. burgdorferi sensu ...
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Routine use of PCR restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media
... a PCR capability was to adopt a PCR-based procedure that would allow us to identify acid-fast isolates growing in BACTEC 12B and 13A ...other PCR-based procedures capable of identifying multiple ...
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Identification of Mycobacterium Species by PCR Restriction Fragment Length Polymorphism Analyses Using Fluorescence Capillary Electrophoresis
... five restriction enzymes to digest amplimers of the 16S rRNA gene hypervariable region that were fluorescently la- beled at one ...endmost restriction fragments of both polymorphic ...
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Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... Our PCR protocol is one of the best alternatives for the former, because we have clearly demon- strated that PCR amplification of the aroA gene is specific for ...
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PCR Restriction Fragment Length Polymorphism Analysis for Identification of Bacteroides spp and Characterization of Nitroimidazole Resistance Genes
... HpaII PCR-RFLP profiles consistent with nimB, but the TaqI PCR-RFLP profiles were inconsistent with reported nim genes (Table 2 and ...gene PCR products indicated that these strains contained a novel ...
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Identification of Oral Peptostreptococcus Isolates by PCR Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes
... this PCR-RFLP method as an alternative and more accurate method than phenotypic meth- ods for species-level identification of clinical isolates tenta- tively identified by culture methods as Peptostreptococcus ...
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Novel PCR Restriction Fragment Length Polymorphism Method for Determining Serotypes or Serogroups of Streptococcus pneumoniae Isolates
... simple PCR system based on portions of the capsu- lation locus because of the diversity of gene arrangement in the capsulation cassettes of different ...similar PCR protocol to amplify the capsulation locus ...
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Molecular Identification of Capnocytophaga spp via 16S rRNA PCR Restriction Fragment Length Polymorphism Analysis
... rRNA PCR-RFLP (36), and 16S rRNA sequence analysis (6, ...rRNA PCR-RFLP) are labor-intensive, costly, and time-consuming and therefore not suitable for most microbiology laboratories, especially when ...
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PCR Restriction Fragment Length Polymorphism for Rapid, Low Cost Identification of Isoniazid Resistant Mycobacterium tuberculosis
... MspA1I PCR-RFLP rapid test described above showed 100% specificity and 80% sensitivity in the routine identifica- tion of isoniazid-resistant ...by PCR-RFLP for three isolates that failed to return results ...
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Rapid Screening of Fluoroquinolone Resistance Determinants in Streptococcus pneumoniae by PCR Restriction Fragment Length Polymorphism and Single Strand Conformational Polymorphism
... quencing. The four-digit code that represents the SSCP profile for an isolate also provides an objective way of recognizing the QRDR mutations associated with that strain. If the SSCP patterns for a ...
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Comparison of Two Target Genes for Detection and Genotyping of Giardia lamblia in Human Feces by PCR and PCR Restriction Fragment Length Polymorphism
... A PCR assay targeting the tpi gene was developed to detect and to genotype Giardia lamblia in human ...described PCR-restriction fragment length poly- morphism (RFLP) assays, which are ...
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Differentiation of Bartonella like isolates at the species level by PCR restriction fragment length polymorphism in the citrate synthase gene
... of PCR amplification was only 2 to 3 h, while sample prepa- ration, amplification, and visualization of amplified products were accomplished in 1 ...for PCR-based anal- yses, as described in this study, ...
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