PCR ribotype 027
Molecular Analysis of Clostridium difficile PCR Ribotype 027 Isolates from Eastern and Western Canada
... of 027 strains in hospitals might include (i) the existing composition and dynamics of circulating ...where PCR ribotype 027/NAP1 has yet to establish itself as a major hospital clone, ...
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Epidemiology of Clostridium difficile infection in hospitalized adults and the first isolation of C difficile PCR ribotype 027 in central China
... difficile ribotype 027; CDC: the Centers for Disease Control; CDI: Clostridium difficile infection; CDT: Binary toxin; CIs: Confidence intervals; CLSI: Clinical and Laboratory Standards Institute; CRE: ...
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Comparative genomic analysis of Clostridium difficile ribotype 027 strains including the newly sequenced strain NCKUH-21 isolated from a patient in Taiwan
... (PCR ribotype 027 or RT027), which is the same strain characterized with different methods has resulted in outbreaks worldwide, although cases in Asia and Latin America were less reported compared ...
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Typing and Subtyping of Clostridium difficile Isolates by Using Multiple Locus Variable Number Tandem Repeat Analysis
... common PCR ri- botype, 001, and two other, emerging PCR ribotypes of ...as PCR ribotype 027, toxinotype III, pulsed-field gel electrophoresis (PFGE) type NAP1, and re- striction ...
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Effectiveness of various cleaning and disinfectant products on Clostridium difficile spores of PCR ribotypes 010, 014 and 027
... difficile PCR ribotypes 010, 014 and ...difficile ribotype 010 does not produce toxins and therefore is unable to cause CDI in ...difficile PCR ribotype 027 is known for its ...strain ...
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Use of Highly Discriminatory Fingerprinting to Analyze Clusters of Clostridium difficile Infection Cases Due to Epidemic Ribotype 027 Strains
... ficile PCR ribotypes 027 (often referred to as North American pulsed-field type 1 [NAP1], or group BI by restriction endo- nuclease analysis [REA]) and 001; these strains were referred to as pulsovars A and ...
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Comparison of Two Capillary Gel Electrophoresis Systems for Clostridium difficile Ribotyping, Using a Panel of Ribotype 027 Isolates and Whole Genome Sequences as a Reference Standard
... QCGE PCR ribotyping. Gel-view results of QCGE-based PCR ribotyping for all 21 reference strains are shown in ...in PCR amplification, causing a decrease in the band signal strength with increasing ...
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Outbreak of Clostridium difficilePCR ribotype 027 the recent experience of a regional hospital
... Results: Fifty-three inpatients met the case definition of C. difficile-associated infection: 55% males, median age was 78.0 years (interquartile range: 71.0-86.0), 75% had co-morbidities, only 15% had a nonfatal ...
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An Enhanced DNA Fingerprinting Service To Investigate Potential Clostridium difficile Infection Case Clusters Sharing the Same PCR Ribotype
... the PCR-ribotyping and MLVA-finger- printing combinations are being adopted (2, 7, 11, 13, ...(notably, 027, 001, 017, 106, and 078) were ...difficile PCR ribotype 027 isolates from two ...
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Predominance of PCR-ribotypes, 018 (smz) and 369 (trf) of Clostridium difficile in Japan: a potential relationship with other global circulating strains?
... type 027 isolate, which was sus- ceptible to new ...to PCR-ribotype 027 ...type 027 have been reported so ...larly, PCR-ribotype 078 ...of PCR-ribotype ...
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In vitro susceptibility of Clostridium difficile to SMT19969 and comparators, as well as the killing kinetics and post-antibiotic effects of SMT19969 and comparators against C. difficile
... different PCR ribotypes (referred to as the genotypically distinct group), 10 PCR ribo- type 001 isolates, 11 PCR ribotype 027 isolates, 10 PCR ribotype 106 iso- lates and ...
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Comparison of Results Generated by Serotyping, Pulsed Field Restriction Analysis, Ribotyping, and Repetitive Sequence PCR Used To Characterize Penicillin Resistant Pneumococci from the United States
... We know from a previous study reported by van Belkum et al. (14) that 28 isolates of S. pneumoniae could be differenti- ated into as few as 7 epitypes by ribotyping or as many as 21 by Rep-PCR using the BOX repeat ...
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Clostridium difficile Genotypes in Piglet Populations in Germany
... detection, PCR ribotyping, and multiple-locus VNTR analysis ...by PCR for cdd3 (which encodes an ABC-type transport system) and characterized by PCRs for the toxin genes tcdA (toxin A; nonrepeating region, ...
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Genomic Epidemiology of a Protracted Hospital Outbreak Caused by a Toxin A Negative Clostridium difficile Sublineage PCR Ribotype 017 Strain in London, England
... All 37 isolates were confirmed to be RT017 by repeat PCR ribotyping. Eight isolates from the UHL temporal clusters were subtyped using multilocus variable-number tandem-repeat analysis (MLVA): six were found to be ...
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Development and Validation of an Internationally-Standardized, High-Resolution Capillary Gel-Based Electrophoresis PCR-Ribotyping Protocol for Clostridium difficile
... Reduced PCR cycling and electrophoresis times combined with the ability to increase batch sizes (using multiple fluorescent detection dyes) represents a rela- tively rapid, high-throughput and low cost means of ...
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Comparison of Four Molecular Typing Methods for Characterization of Corynebacterium diphtheriae and Determination of Transcontinental Spread of C diphtheriae Based on BstEII rRNA Gene Profiles
... Transcontinental epidemiology. The results show that the Eastern European epidemic was caused by strains of ribotypes D1, D4, and D7. The cases of diphtheria imported into Fin- land, Germany, and the United Kingdom may ...
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A Role for Tetracycline Selection in Recent Evolution of Agriculture-Associated Clostridium difficile PCR Ribotype 078
... Although multiple lines of evidence indicate a role for tetracycline selection in the recent evolutionary history of RT078, the possibility exists that further lineage-specific genetic changes (unrelated to tetracycline ...
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A role for tetracycline selection in recent evolution of agriculture associated clostridium difficile PCR ribotype 078
... Although multiple lines of evidence indicate a role for tetracycline selection in the recent evolutionary history of RT078, the possibility exists that further lineage-specific genetic changes (unrelated to tetracycline ...
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A Role for Tetracycline Selection in Recent Evolution of Agriculture Associated Clostridium difficile PCR Ribotype 078
... ABSTRACT The increasing clinical importance of human infections (frequently se- vere) caused by Clostridium difficile PCR ribotype 078 (RT078) was first reported in 2008. The severity of symptoms (mortality ...
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Molecular Epidemiology of Endemic Clostridium difficile Infection and the Significance of Subtypes of the United Kingdom Epidemic Strain (PCR Ribotype 1)
... difficile PCR ribotype 1 isolates can be subtyped by both ran- domly amplified polymorphic DNA and PFGE fingerprinting techniques and by determination of their susceptibilities to ...difficile PCR ...
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