Peptide nucleic acid
Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... with peptide nucleic acid (PNA) probes for identification of Staphylococcus aureus directly from positive blood culture bottles that contain gram-positive cocci in clusters (GPCC) is ...
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Design and Evaluation of Peptide Nucleic Acid Probes for Specific Identification of Candida albicans
... rRNA-directed peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) probes, P-Ca726 (targeting a novel region of the ribosome) and P-CalB2208 (targeting a previously reported region), ...
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Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens
... detects nucleic acid sequences by means of a fluorescently labeled probe that hybridizes specifically to its complementary target sequence within the intact cell ...recently, peptide nucleic ...
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Peptide Nucleic Acid Based Array for Detecting and Genotyping Human Papillomaviruses
... We describe a novel array for accurate and reliable genotyping of human papillomavirus (HPV) using peptide nucleic acid (PNA) probes. In order to exploit the superior hybridization properties of PNA ...
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Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi probe real time PCR method into sputa samples
... Peptide nucleic acids (PNAs) are artificially synthesized DNA analogues with an uncharged peptide ...and peptide bond-linked backbone ...novel peptide nucleic acid (PNA) ...
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<p>Preparation and SPECT/CT Imaging of <sup>177</sup>Lu-Labeled Peptide Nucleic Acid (PNA) Targeting CITED1: Therapeutic Evaluation in Tumor-Bearing Nude Mice</p>
... 15 Peptide nucleic acid (PNA) was developed from oligonucleotides (ODN), which can block the function of RNA polymerase structu- rally and thus interferes with gene transcription and down- regulates ...
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Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
... using peptide nucleic acid (PNA) probes for differentiation between species of the Mycobacterium tuberculosis complex (MTC) and nontubercu- lous mycobacteria (NTM) in acid-fast ...
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Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles
... uses peptide nucleic acid (PNA) probes for identification of Candida albicans directly from positive-blood-culture bottles in which yeast was observed by Gram staining (herein referred to as ...
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A Peptide Nucleic Acid to Reduce Type I Collagen Production by Fibroblast Cells
... In this study, we prepared a peptide nucleic acid (PNA) against the gene coding for the human alpha 1 chain of type I collagen. This PNA was incorporated into normal human fibroblast cells by ...
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Peptide Nucleic Acid (PNA) Antisense Effects in Escherichia coli
... Peptide nucleic acid (PNA) is a DNA mimic with nucleobases attached to a pseudo-peptide backbone ...The peptide backbone provides superior hybridisation (10), resistance to enzymatic ...
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Peptide Nucleic Acid Array for Detection of Point Mutations in Hepatitis B Virus Associated with Antiviral Resistance
... The detection of antiviral-resistant hepatitis B virus (HBV) mutations is important for monitoring the response to treatment and for effective treatment decisions. We have developed an array using peptide ...
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A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array
... Abstract: E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes ...
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Identification of Fusarium Species in Formalin Fixed and Paraffin Embedded Sections by In Situ Hybridization Using Peptide Nucleic Acid Probes
... Fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of Fusarium from Aspergillus and Scedosporium is particularly difficult because these fungi may induce similar ...
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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
... on peptide nucleic acid (PNA) technology coupled to immunofluorescence microscopy (PNA-FISH) for the specific detection at a single cell level of BCR-ABL T315I mutation thus improving both, ...
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An Introduction to Peptide Nucleic Acid
... It is tempting to regard PNA as a DNA analogue, but as it’s chemical structure shows (Figure 1), it is more correctly a protein and peptide like molecule, and working with PNA and when developing new procedures ...
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Synthesis of 2-thiouracil and 2-thiothymine peptide nucleic acid monomers compatible with fmoc-based oligomerization
... sugar. Nucleic acid strands can undergo hybridization between complementary base pairs adenine and thymine, and cytosine and guanine to form a double stranded ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... cleic acid greatly helped us study biological evolution, gene analysis, disease diagnosis, as well as drug dis- ...clear acid. Among these analogs, peptide nucleic acid (PNA) and locked ...
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<p>Biological Activity Of miRNA-27a Using Peptide-based Drug Delivery Systems</p>
... ● miRNA mimic transfection control with Dy547 (FluoNTC) comprising the sequence CUCUUUCUA GGAGGUUGUGA 5´ Dy547 (MW 14 566.9 g/mol) to perform fl uorescence experiments in-vitro RNase-free water (VWR, Vienna, Austria) was ...
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
... Peptide Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and ...synthetic nucleic acids and artificial analogues, PNA has been extensively ...
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Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase Negative Staphylococci Directly from Blood Culture Bottles in Less than 30 Minutes
... There are a number of methods currently used for identifica- tion of staphylococci in clinical samples, and they vary greatly in accuracy, speed, and cost. The tube coagulase test detects the pres- ence of free coagulase ...
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