Phi X174
Cloned bacteriophage phi X174 DNA sequence interferes with synthesis of the complementary strand of infecting bacteriophage phi X174.
... Analysis of cells that contain plasmids with the "reduction sequence" showed that i the adsorption of the phages to the host cells is normal, ii in a single infection cycle much less pha[r] ...
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Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.
... Howsince rho activity in a rho mutant is already at ever, under conditions where the major DNA a marginal level for survival 4, 11, the mutant repair mechanisms are functioning, the actu[r] ...
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Electrophoretic characterization of intracellular forms of bacteriophage phi X174 DNA: identification of novel intermediate of altered superhelix density.
... Electrophoretic analysis of RF DNA after controlled denaturation suggests the existence of four populations of closed circular RF: i molecules of native superhelix density RFI; ii a popu[r] ...
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Cleavage of phi X174 single-stranded DNA by gene A protein and formation of a tight protein-DNA complex.
... Less than 0.5% and 0.1% of the total 32p label remained resistant to DNase I and micrococcal nuclease digestion, respectively, measured as trichloroacetic acid-precipitable The introduct[r] ...
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Mechanism of replication of bacteriophage phi X174 XX. Sensitivity of nascent DNA to single-strand-specific nucleases.
... When a mixture of 3H-labeled nascent viral DNA labeled late in infection and 32P-labeled non-nascent 4X viral strand DNA molecules of approximately the same size was exposed to spleen ex[r] ...
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Mutations in the J-F intercistronic region of bacteriophages phi X174 and G4 affect the regulation of gene expression.
... Comparison of the amount of gene D protein synthesized in infected cultures to the amounts of capsid proteins F, G, and H indicated a consistent relative decrease of gene D protein in al[r] ...
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Selective cloning of a DNA single-strand initiation determinant from phi X174 replicative-form DNA.
... To identify SS initiation determinants ssi that might be contained within either the viral or complementary strand of ,X174 duplex DNA, we cloned restriction fragments of 4X174 RF into t[r] ...
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Expression of the cloned bacteriophage phi X174 A* gene in Escherichia coli inhibits DNA replication and cell division.
... Upon induction of expression, DNA synthesis in Escherichia coli carrying the recombinant plasmid is severely inhibited; however, these same cells permit 1-galactosidase induction at a ra[r] ...
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DNA synthesis in Escherichia coli cells infected with gene H mutants of bacteriophage phi X174.
... Thus, it is likely that in the H mutant-infected cells, full-length ssDNA synthesis is occurring, and the various H mutant phages have different relative successes in incorporating these[r] ...
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Bacteriophage phi X174-specific mRNA synthesis in cells deficient in termination factor rho activity.
... We found that i normal termination sites of 4X174 mRNA synthesis are recognized in nitA cells, ii the rho mutation relieves polar effects caused by nonsense mutations in the phage genome[r] ...
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Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage phi X174.
... In an effort to elucidate the possible protein interactions between gpB and other phage proteins, we undertook a second site analysis of csB polypeptides generated by g[r] ...
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Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.
... Isolation of second-site suppressors of a cs exter- nal scaffolding protein: All of the second-site sup- pressors, except one, conferred amino acid substitu- tions in [r] ...
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Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.
... The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA.. [r] ...
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Evolution of bacteriophage phi X174. IV. Restriction enzyme cleavage map of St-1.
... Digestion of HaeIII fragments with HincII Fragments obtained by digesHaeIII fragments tion with HincIIa HaeIIIl HincII7 263 Uncut The size of each fragment produced by digestion by the s[r] ...
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Application of Arrhenius kinetic theory to viral eclipse: selection of bacteriophage phi X174 mutants.
... Thus, the ratelimiting step in the intracellular development of the mutant is the same as that for the wild type at all temperatures between 40 and 250C, and only one Arrhenius plot is r[r] ...
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Mechanism of replication of bacteriophage phi X174 XIX. Initiation of phi X174 viral strand DNA synthesis at internal sites on the genome.
... Initiation of 4X174 Viral Strand DNA Synthesis at Internal Sites on the Genome Received 3 June 1981/Accepted 16 November 1981 Bacteriophage PX174 viral strand DNA molecules shorter than [r] ...
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Role for the J-F intercistronic region of bacteriophages phi X174 and G4 in stability of mRNA.
... Termination sites in class 2 mutants for transcripts IX and XIII could not be estimated because of the lack of recognizable IX and XIII peaks in the gel profiles, but the ratios of genes[r] ...
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Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
... The efficiency of plaque formation for all strains with gene E nonsense mutations, as well as that for the ribosome-binding site mutants, was higher on bile saltslysozyme plates 100% tha[r] ...
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Growth and DNA synthesis of bacteriophage phi x174 in a dnaP mutant of Escherichia coli.
... TPG-CA medium was described by Sakai and Komano 20, and starvation 650 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest 4X174am3trD, a temperature-resistant mutant of ba[r] ...
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DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.
... We conclude that the loss of normal transfecting activity upon methylation is not simply due to extensive modification of the complementary strand opposite the site which is specifically[r] ...
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