Plasmid maintenance
The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance
... virulence plasmid pYV increased up to 6-fold compared with the infecting ...pYVe plasmid partition via SpyA. Plasmid copy number and efficient virulence plasmid partition are likely to be ...
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EBP2, a Human Protein That Interacts with Sequences of the Epstein-Barr Virus Nuclear Antigen 1 Important for Plasmid Maintenance
... the plasmid. After 2 weeks the cells were harvested, and plasmid DNA was isolated and analyzed by Southern ...oriP-containing plasmid that expressed EBNA1 was maintained in the cells, while the ...
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A bacteriocin gene cluster able to enhance plasmid maintenance in Lactococcus lactis
... In this work, we have assessed the use of the Lcn972 gene cluster to improve the segregational stability of lac- tococcal plasmids without the aid of antibiotics. Lcn972 is a non-lantibiotic bacteriocin that selectively ...
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Suppression of STAT-1 Expression by Human Papillomaviruses Is Necessary for Differentiation-Dependent Genome Amplification and Plasmid Maintenance
... The question arises why restoring STAT-1 levels to those seen in HFKs is so deleterious to viral replication since in- creased expression in normal cells has minimal effects on cell viability. STAT-1 along with STAT-2 ...
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Plasmid maintenance in Escherichia coli K 12
... functions. The first accomplishes the circularization of linear Pi DNA upon entry into a host bacterium. This is conditional on the Pi phage genome containing two loxP sites, normally the result of terminal redundancy ...
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Bovine papillomavirus type 1 3' early region transformation and plasmid maintenance functions.
... State of mutant BPV-1 viral DNAs in mouse C127 cellsa Plasmid Plasmid p142-6 p593-6 p743-23 p631-1 p771-1 p772-1 p448-5 p327-25 p830-1 ORFs affected State of DNAcells in transformed Extr[r] ...
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Epstein-Barr Virus Nuclear Antigen 1 Replication and Segregation Functions in Nasopharyngeal Carcinoma Cell Lines
... 3. Plasmid maintenance and replication assays in CNE2E ...OriPE plasmid as indicated, harvested after 3 to 56 cell doublings (3 to 56 days), and processed as described in the legend for ...
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Identification of cellular factors that bind specifically to the Epstein-Barr virus origin of DNA replication.
... To identify cellular proteins possibly involved in EBV plasmid maintenance, protein-DNA gel retardation experiments and DNase I footprinting were performed, * using HeLa cell nuclear ext[r] ...
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The bovine papillomavirus constitutive enhancer is essential for viral transformation, DNA replication, and the maintenance of latency.
... Since E2TA can transactivate the expression of most of the early region ORFs in transient assays 20, 21, 27, 36, 45, 47 and is essential for transient and stable plasmid maintenance 15, [r] ...
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Replacement of the Epstein-Barr Virus Plasmid with the EBER Plasmid in Burkitt's Lymphoma Cells
... Epstein-Barr virus (EBV) is present in tumor cells of more than 90% of cases of Burkitt’s lymphoma (BL) in areas of endemicity in equatorial Africa and New Guinea, as well as in tumor cells of about 10% of sporadic cases ...
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Dictyostelium discoideum Nuclear Plasmid Ddp5 Is a Chimera Related to the Ddp1 and Ddp2 Plasmid Families
... in plasmid maintenance, it is possible that it confers a selective advantage to plasmid-bearing cells (Table ...on maintenance, autonomous replication, or copy number of the p94d5 ...
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Rapid compensatory evolution promotes the survival of conjugative plasmids
... for plasmid maintenance are surprisingly stringent; amelioration mutations must have both large fitness effects and occur at a high rate in order to stabilize plasmids in bacterial ...of plasmid ...
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Functional limits of oriP, the Epstein-Barr virus plasmid origin of replication.
... Deletions that left six or fewer 30-bp repeats in an EBV plasmid or plasmids reconstructed with up to five copies of a 30-bp repeat oligonucleotide were defective for plasmid maintenance[r] ...
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Genetically Validated Drug Targets in Leishmania: Current Knowledge and Future Prospects
... forced plasmid shuffle to assess gene ...of plasmid shuffle in that a MPK4 mutant with altered ATP binding properties, predicted to have reduced protein kinase activity, could replace wild-type MPK4, ...
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Severe Acute Respiratory Syndrome Coronavirus Open Reading Frame (ORF) 3b, ORF 6, and Nucleocapsid Proteins Function as Interferon Antagonists
... a plasmid construct containing a firefly luciferase gene under the control of a promoter with three IRF-3 binding sites ...luciferase plasmid, and with a plasmid that constitutively expresses Renilla ...
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Recent emergence of new variants of Yersinia pestis in Madagascar
... The three new variants differed from the original strains not only in their ribotypes but also in their pulsotypes (strains of ribotype Q and R) and plasmid profiles (strains of ribotype Q and T). Whether there is ...
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The analysis of plasmid rearrangements observed in the soil bacterium OR168 after the introduction of transposon Tn5 : a thesis presented in fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University
... LIST OF FIGURES Structure of Tn5 1.5.1 Models for transposition mechanisms 1.5.2 3.2.1 Eckhardt plasmid profiles of reference strains with known plasmid sizes compared with plasmid profi[r] ...
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1782-06.pdf
... Identification of SARS-CoV ORF 3b, ORF 6, and N proteins as interferon antagonists. To determine whether the structural or accessory proteins of SARS-CoV were interferon antago- nists, the proteins were screened using an ...
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Research of protectionism in shipping policy
... In pursuing a balanced maintenance system, it is therefore necessary to design a scheme which will unite scheduled maintenance, corrective maintenance and condition based maintenance in [r] ...
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Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria
... weight plasmid while some had both low and heavy molecular weight plasmid and are multi-drug ...post plasmid curing resistance pattern revealed that multiple antibiotic resistance observed in these ...
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