PMA/Ionomycin
Murine CD4+CD25- cells activated in vitro with PMA/ionomycin and anti-CD3 acquire regulatory function and ameliorate experimental colitis in vivo
... Subsequently PMA/ionomy- cin and IL-2 were added to the cell ...of PMA/ionomycin ac- tivation step on CD4 + CD25 - cells resulted in significant decrease in cell viability (data not shown) which con- ...
9
Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken
... In avian species, the immunological toolbox for studying cytokine production of different T-cell subsets, especially antigen-specific T cells, is still limited. Flow cytometry- based intracellular cytokine staining is a ...
15
Three-Color Flow Cytometry Detection of Intracellular Cytokines in Peripheral Blood Mononuclear Cells: Comparative Analysis of Phorbol Myristate Acetate-Ionomycin and Phytohemagglutinin Stimulation
... In conclusion, PHA stimulates a wider range of intracellular cytokines in human PBMCs than does PMA-ionomycin and is particularly effective for activation of monocytes for cytokine production. Moreover, PHA ...
11
A real time view of the TAR:Tat:P TEFb complex at HIV 1 transcription sites
... HEXIM-1 or 7SK, which together form a complex that inactivates P-TEFb ([13]; Figure 2). To test whether the accumulation of Tat at the HIV-1 transcription site depended on its interaction with Cyclin T1, we analyzed the ...
5
AMPK-dependent and independent effects of AICAR and compound C on T-cell responses
... measured intracellular cytokine production under both conditions (Figure 4 and Figure S5). Our results suggest that AMPK affects T cell cytokine production via both pathways. Intriguingly, addition of either AICAR or ...
13
Collagen density regulates the activity of tumor-infiltrating T cells
... observed in cells isolated from four additional healthy donors. A panel of six transcripts, which were significantly regulated by collagen-density in the RNA sequencing ex- periments (two upregulated and four ...
15
S-nitrosoglutathione modulates CXCR4 and ICOS expression
... under PMA/Ionomycin treatment. PMA/Ionomycin treatment up-regulates the cell surface expression of inducible co-stimulator (ICOS), which is a member of the CD28 family and is induced on ...
7
Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae
... Following PMA/ Ionomycin stimulation, IL-17A-producing γδ T cells could be identified (Figure 5 ; Additional file 8 , bottom panel, arrowhead 2), although IL-17A producing cells iso[r] ...
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Hepatitis C Virus Infection of Human T Lymphocytes Is Mediated by CD5
... To identify the reasons behind the discrepant susceptibilities to HCV infection in the T cell lines, we reevaluated their phenotypic characteristics and assessed expression of CD81, as well as that of selected innate ...
13
Integration of kinase and calcium signaling at the level of chromatin underlies inducible gene activation in T cells
... RNA was extracted from Jurkat T cells as outlined above, either untreated or stimulated with PMA/ionomycin for 3 h. RNA was assayed using a custom- made human nCounter kit (NanoString Technologies), which ...
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Immunomodulatory effect of water soluble extract separated from mycelium of Phellinus linteus on experimental atopic dermatitis
... with PMA/ ionomycin stimulation, under the antigen specific stimula- tion by mite extract, WA also significantly decreased the expression levels of pathogenic cytokines (IL-4, IL-13 and IFN-γ) and ...
12
Human Immunodeficiency Virus Type 1 Nef Mediates Sustained Membrane Expression of Tumor Necrosis Factor and the Related Cytokine LIGHT on Activated T Cells
... with PMA and ionomycin, cell surface levels of TNF were increased twofold in ...of PMA-ionomy- cin (51% of the Nef-GFP-transfected cells expressed TNF ver- sus 26% of GFP-transfected cells) ...of ...
7
Agonist redirected checkpoint, PD1-Fc-OX40L, for cancer immunotherapy
... Primary human T cells were also stimu- lated with PMA/PHA/ionomycin to upregulate OX40 (Additional file 4 : Figure S3C), and PD1-Fc-OX40L was shown to have increased binding to activated[r] ...
16
Surfactant Protein-A inhibits Aspergillus fumigatus-induced allergic T-cell responses
... The mechanism of T cell susceptibility to inhibition by SP- A needs further clarifications. While the inhibitory effects of SP-A have been demonstrated in antigen-stimulated human PBMC [33] and more recently, in a murine ...
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Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT
... duction of these cytokines; with isolated soluble LIGHT antibody preincubation resulted in almost complete inhibition of up-regulation of ICAM-1 and IL-8. Membrane-bound LIGHT of stimulated PBMC was shown to induce ...
10
Vδ2+ and α/ß T cells show divergent trajectories during human aging
... Stimulation of α/β T cells using the polyclonal activators, PMA and ionomycin, showed an increased of TNF-α and IFN-γ double positive α/β T cells in the elderly compared to the young.. P[r] ...
13
Increased expression of CD40 ligand on systemic lupus erythematosus lymphocytes
... with PMA and ionomycin, T cells from normal sub- jects, SLE patients, and patients with systemic inflammatory disease show comparable high level expression of CD40L at 6 h of culture and similar kinetics of ...
13
Pilomyxoid Astrocytoma: Expanding the Imaging Spectrum
... Contrast-enhanced T1 sequences were available for review in all patients and showed the following patterns: 8 of 21 (38%) showed solid uniform enhancement, 6/21 (29%) showed heterogeneous enhancement with rim ...
6
SPECTROPHOTOMETRIC DETERMINATION OF CABERGOLINE IN TABLET DOSAGE FORMS
... with PMA (first step) and estimating the PMA released (with acetone from its adduct), by reducing it with Co(II)-EDTA complex (second ...of PMA, cobalt nitrate, EDTA, heating time for the maximum ...
6
Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
... of PMA on K562 and THP1 leukemia cells were studied, and the results showed that PMA treatment significantly increased cell adhesion with extracellular matrix proteins, bone marrow stromal cells, and human ...
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