Poliovirus RNA replication complex
Generation of Unique Poliovirus RNA Replication Organelles
... post-RNA replication role in PV infection (6, ...of RNA replication in association with LC3-labeled, double-membraned structures ...into replication membranes late in ...tion, ...
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Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis
... with poliovirus (PV) RNA replication have contributed to new insights into this complex ...viral RNA (vRNA) in various steps leading to production of new vRNA ...template-dependent ...
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Rescue of Defective Poliovirus RNA Replication by 3AB-Containing Precursor Polyproteins
... individual RNA replication complexes that are physically separated from each other in the ...the replication complexes may be processed or assembled such that they are no longer competent for subunit ...
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Formation of the Poliovirus Replication Complex Requires Coupled Viral Translation, Vesicle Production, and Viral RNA Synthesis
... viral replication complexes formed after subse- quent virus ...viral replication proteins, cells were trans- fected with a plasmid, pE5PV⌬P1, encoding the entire PV P2 and P3 regions, fused to the EMCV IRES ...
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Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro
... genomic RNA within replica- tion complexes contained in the cytoplasm of the infected ...for RNA replication and encapsidation of the progeny RNA ...strand RNA genome of PV yields a ...
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Complex Dynamic Development of Poliovirus Membranous Replication Complexes
... membranous replication complexes of several positive- strand RNA viruses was investigated by electron tomographic methods similar to those used in the studies reported ...positive-strand RNA viruses, ...
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Poliovirus infection and expression of the poliovirus protein 2B provoke the disassembly of the Golgi complex, the organelle target for the antipoliovirus drug Ro-090179.
... viral replication complexes hosted in Golgi- or ER-derived membrane rosettes, and thus inhibit viral RNA ...Golgi complex, and induce the swelling of endosomes and lysosomes ...to poliovirus ...
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Differential Rescue of Poliovirus RNA Replication Functions by Genetically Modified RNA Polymerase Precursors
... the RNA replication properties of poliovirus transcripts harboring chimeric RNA polymerase sequences representing suballelic exchanges between poliovirus type 1 (PV1) and coxsack- ...
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Inhibition of Poliovirus-Induced Cleavage of Cellular Protein PCBP2 Reduces the Levels of Viral RNA Replication
... of poliovirus virion RNA (lane 1) produces viral proteins, both mature and precursor forms, labeled by the incorporation of [ 35 ...poly(rC) RNA, levels of translation are decreased (lane 3) as ex- ...
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Hijacking Components of the Cellular Secretory Pathway for Replication of Poliovirus RNA
... poliovirus replication complex formation, we studied viral RNA replication in cells ectopically expressing GBF1 and ...virus replication to the downregulation of a particular ...
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Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication
... chimeric poliovirus in which the predicted N-terminal amphipathic helix in protein 2C was replaced with the corresponding region from HRV14 produced very small plaques on HeLa cell monolayers and displayed delayed ...
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Identification of a cis-Acting Replication Element within the Poliovirus Coding Region
... in replication for these or other picornavirus structures has yet to be obtained, several other positive-sense RNA viruses contain CREs for which a replication function has been defined (re- viewed ...
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Functional Coupling between Replication and Packaging of Poliovirus Replicon RNA
... genome replication and packaging has been documented for a few other viruses that are not closely related to ...ribonucleoprotein complex that includes the viral reverse transcriptase is required for ...
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Initiation of Poliovirus Negative-Strand RNA Synthesis Requires Precursor Forms of P2 Proteins
... in poliovirus- infected cells (52) appears to be consistent with a 2BCP3 requirement for formation of functional PIRCs that efficiently synthesize negative-strand ...support poliovirus replication ...
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Reversible dissociation of the poliovirus replication complex: functions and interactions of its components in viral RNA synthesis.
... Membrane-bound replication complexes containing transcriptionally active replicative intermediates (RI) can be isolated from poliovirus-infected HEp-2 cells and consist of rosette-like structures of ...
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Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
... viral RNA replication in the HeLa S10 translation-replication ...of poliovirus RNA replication without affecting the polymerase elongation reaction (32, ...of RNA ...
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Poliovirus 5′-Terminal Cloverleaf RNA Is Required in cis for VPg Uridylylation and the Initiation of Negative-Strand RNA Synthesis
... Proximal RNA termini: a common strategy for RNA animal viruses? RNA animal viruses appear to utilize communication between the 5⬘ and 3⬘ termini of their viral RNAs as a common strategy to regulate ...
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Requirements for RNA Replication of a Poliovirus Replicon by Coxsackievirus B3 RNA Polymerase
... ribonucleoprotein complex with the first ⬃100 nt of the 5⬘ end of PV1 plus-strand ...ternary complex with 5⬘ proximal sequences, which fold into a CL structure (1, 2, 5, 14, ...CL RNA representing ...
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Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.
... Evidence from In Vitro RNA Synthesis that PolyA of the Poliovirus Genome is Genetically Coded Received for publication 3 July 1975 A crude replication complex has been isolated from poli[r] ...
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Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg
... picornaviral RNA replication is that RNA transcription is confined to the viral template and does not involve cellular ...served RNA structural features in the 3⬘NTR were predicted to posses ...
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