polymerase chain reaction method
Detection of E. coli O157:H7 in Meat Using Polymerase Chain Reaction Method and Culture Method
... In the present study, 28 samples (10%) were identified as E. coli O157:H7 based on the lack of sorbitol fermentation. The results of PCR procedure demonstrated that out of the 28 samples, 21 cases were identified as E. ...
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A Nested Allele-Specific Multiplex Polymerase Chain Reaction Method for the Detection of DRD2 Polymorphisms
... genotyping method has been described that uses microchip electrophoresis for the analysis of PCR products for sizing, mutations, or ...this method can be used in detecting the methylated p16 gene in cancer ...
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RAPID POLYMERASE CHAIN REACTION METHOD FOR DETECTION OF VIBRIO CHOLERAE IN FOOD AND THEIR CYTOTOXIC EFFECT
... The polymerase chain reaction was used to amplify a region of DNA that lies between two regions of known sequence. In this technique double stranded target DNA is denatured to provide single stranded ...
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Development of a reverse transcription polymerase chain reaction method for yellow fever virus detection
... Thirty-three clinical samples were tested with the standardized reaction. Results. The expected amplicon was obtained in 25 out of 33 samples analyzed using this approach, and 2 more samples tested positive after ...
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Rapid Detection of Shigella flexneri on Egg Samples by the Real Time Polymerase Chain Reaction Method
... RT-PCR method to detect and quantify the Shigella flexneri bacteria in egg samples with the ipaH target ...RT-PCR method of ...culture method. Based on the results, it can be concluded that the RT- ...
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Serotyping of Listeria monocytogenes Isolates from Women with Spontaneous Abortion Using Polymerase Chain Reaction Method
... Background: Listeria monocytogenes infection during pregnancy may cause spontaneous abortion or stillbirth, or the birth of babies with neonatal meningitis. The aim of this study was to serotype L. monocytogenes isolated ...
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THE advent of the polymerase chain reaction in the
... a method not used in any of the herpetofaunal studies reviewed here, but certainly worth future exploration as RADseq is typically lower in cost when compared to the aforementioned ...
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Polymerase chain reaction on a viral nanoparticle
... a method to link a structural element, namely the bacteriophage M13, to an easily programmed assembly element, ...simple method for generating hybrid nanoparticles that contain extended DNA assembly ...
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SCIENTIFIC CONCEPTS OF POLYMERASE CHAIN REACTION (PCR)
... http://dx.doi.org/10.13040/IJPSR.0975-8232.5(8).3086-95 Polymerase Chain Reaction (PCR) is one of the method for the amplification of DNA that has varied applications in many and diverse areas ...
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A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.
... C. nuclear DNA sequences, would give rise to a unique mi- cronuclear PCR fragment when whole cell DNA was used as template. Figure 5 shows the arrangement of this region and[r] ...
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Polymerase Chain Reaction as Screening Method for Group B Streptococcus in Third Trimester of Pregnancy
... DISCUSSION Early onset GBS disease is the leading infectious cause of morbidity and mortality among infants in developed countries. Currently, intrapartum an- tibiotic prophylaxis is the only effective method to ...
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RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]
... detection method in shrimp by ...protocol method to detect Salmonella invA gene was conducted using six different annealing temperatures (59, ...detection method has a DNA detection limit of ...
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Rapid sexing of selected Galliformes by polymerase chain reaction
... Sexing one-day-old chicks can be usually accom- plished by one of the two methods: (1) vent sexing or (2) feather sexing. Each method deals with some difficulties making its use in small flocks unsuit- able. Vent ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... QPCR method is available, these experiments have been extended to allow measurement o f low concentrations of aerosols within the cabinet and to determine whether there is a good correlation between cells released ...
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Evaluation of Polymerase Chain Reaction for Diagnosis of “Tuberculous Pleurisy”
... • Direct smear of pleural fluid has limited diagnostic value because of the need for at least 10,000 / ml of organisms (8). Therefore, in the best case scenario, it is positive in less than 10% of cases (9-14). Culture ...
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Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... Step 5: The answer is ‘YES’ if any path remains, otherwise ‘NO’. Adleman proved that this algorithm can be implemented in molecular level. Adleman used a set of 20-mer oligonucleotides, or oligos, to encode each vertex ...
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Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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A simple and rapid method for detection of Trypanosoma evansi in the dromedary camel using a nested polymerase chain reaction
... Polymerase chain reaction (PCR) A stock buffered solution containing 250 µl 10× PCR buffer, 100 µl of magnesium chloride, ...DNA polymerase (Perkin Elmer) was used at a concentration of ...
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Polymerase chain reaction: trenches to benches
... The application of molecular diagnostic techniques to the diagnosis of infectious disease is based on the identification of unique "signature sequences" in the DNA or RNA of a pa[r] ...
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