Pooling all samples for multiplex-sequencing
Pooling samples for “top-down” molecular exposomics research: the methodology
... individual samples with- out pooling may prove a sufficient ...sample pooling is a valuable tool for reducing costs, workload and the need for large individual sample ...tion samples, followed ...
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A Stochastic Max Pooling Strategy for Convolutional Neural Network Trained by Noisy Samples
... test samples contain lots of ...max pooling. Unlike the traditional max pooling, the proposed strategy first ranks all the values in each receptive field, and then selects a random value from ...
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Pooling of Urine Samples for Screening for Neisseria gonorrhoeae by Ligase Chain Reaction: Accuracy and Application
... serves. Pooling of samples from patients in a population where the prevalence of gonorrhea may be 20% or greater is not advised and would be minimally cost ...The pooling algorithm would be cost ...
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Completing bacterial genome assemblies with multiplex MinION sequencing
... ligation sequencing kit (SQK-LSK108) with the native barcoding expansion kit ...DNA sequencing was followed with the following modifications to maximize DNA ...library pooling, the amount of DNA to ...
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High-Throughput Multiplex Sequencing to Discover Copy Number Variants in Drosophila
... of samples among the sequenced ...of samples postsequencing. We designed primers to test all 16 dinucleotide combinations for their efficiency in library preparation ...by multiplex microbial ...
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A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
... by sequencing only one subclone per bisulfite conversion reaction (see Section ...PCR, pooling potentially mixed populations of alleles together, as previously described in ...
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On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments
... of pooling RNA samples in RNA-seq experiments was subsequently assessed based on both empirical and simulated RNA-seq ...that pooling strategies offer a number of benefits. In par- ticular, ...
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Data Analysis of Multiplex Sequencing at SOLiD Platform: A Probabilistic Approach to Characterization and Reliability Increase
... New sequencing technologies such as Illumina/Solexa, SOLiD/ABI, and 454/Roche, revolutionized the biological ...particular sequencing type, known as multiplex run, which enables the sequencing ...
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Whole-genome sequencing in an autism multiplex family
... pilot sequencing analysis on patients from multiplex ...DNA samples were avail- able for all subjects, except for one unaffected ...whole-genome sequencing data on the two ...genotyped ...
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Testing for Neutrality in Samples With Sequencing Errors
... that sequencing errors are mostly ...of sequencing errors, neutrality tests based on the frequency spectrum reject ...despite sequencing errors. All in all, we show that even though ...
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Preparing Samples for Sequencing Genomic DNA
... and/or all parts thereof, the instructions in this guide must be strictly and explicitly followed by experienced ...personnel. All of the contents of this guide must be fully read and understood prior to ...
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Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
... of samples) were noted, but 46 were due to nonreproducible technical problems or insufficient growth of the ...pneumococci. All of the isolates grew well for a second test, and therefore, the culture medium ...
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Multiplex Amplicon Sequencing For Microbe Identification In Community-based Culture Collections
... metagenomic sequencing has revealed a vast microbial diversity associated with ...amplicon sequencing based on two-step PCR amplifications with tagged primers for plates, rows, and columns allowed the ...
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Utility of a Multiplex PCR Assay for Detecting Herpesvirus DNA in Clinical Samples
... Assay controls. A negative control consisting of nuclease-free water was in- cluded in every assay. Each positive control virus used in the assay (HSV-2, CMV, and VZV) was prepared from a known isolate serially titrated ...
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Direct Sequencing of Cryptosporidium in Stool Samples for Public Health
... Thirty-nine species of Cryptosporidium have been described at the time of writing (Table 1), but not all cause human disease. The vast majority of human cryptosporidiosis is caused by the zoonotic species ...
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Clinical sequencing using a next-generation sequencing-based multiplex gene assay in patients with advanced solid tumors
... next-generation sequencing (NGS) technologies have enabled physi- cians to test for genomic alterations in multiple cancer-related genes at once in daily clinical ...clinical sequencing using an NGS-based ...
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Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing.
... partial sequencing for ...RNA sequencing (DRS) using nanopore sequencing technology for fast whole-genome sequencing of viruses directly from clinical ...by sequencing the corresponding ...
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Multiplex PCR and next generation sequencing for the non invasive detection of bladder cancer
... urine samples, and even on samples where the amount of DNA extracted from the urine pellets is too low to quantify by conventional means ( < ...10 samples which did not give suffi- cient read depth ...
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Development of a microfluidic unit for sequencing fluid samples for composition analysis
... ‘cleaning reverse flows’ generated in the valves in their ‘closed’ state. Another potential area of contamination was the ‘open’ state valve. In contrast to a closed mechanical valve, in which the fluids are separated by ...
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Hybrid selection for sequencing pathogen genomes from clinical samples
... clinical samples at scale due to the gross excess of host DNA typically ...augment sequencing coverage depth. However, this strategy can be costly for all but the most lightly con- taminated ...size, ...
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