Processivity factor
A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor
... Fig. 1. Model of the replication fork of phage T7. The replisome consists of DNA polymerase (gp5), the processivity factor thioredoxin (trx), the hexameric helicase 兾 primase (gp4), and the ssDNA-binding ...
7
Role of the Specific Interaction of UL112-113 p84 with UL44 DNA Polymerase Processivity Factor in Promoting DNA Replication of Human Cytomegalovirus
... The human cytomegalovirus (HCMV) UL112-113 region encodes four phosphoproteins with common amino termini (p34, p43, p50, and p84) via alternative splicing and is thought to be required for efficient viral DNA ...
13
Downregulation of Poly(ADP-Ribose) Polymerase 1 by a Viral Processivity Factor Facilitates Lytic Replication of Gammaherpesvirus
... viral processivity factor was conserved among gammaherpesviruses, Western blot and immunofluores- cence assays were performed with HEK293T cells transiently transfected with MHV-68 mPF (24) ...viral ...
8
Nuclear Transport of Epstein-Barr Virus DNA Polymerase Is Dependent on the BMRF1 Polymerase Processivity Factor and Molecular Chaperone Hsp90
... polymerase processivity factors of many human herpesviruses such as UL42 of herpes simplex virus 1 (HSV-1) (32), BMRF1 of EBV (33), UL44 of human cytomega- lovirus (HCMV) (34), PF-8 of Kaposi’s sarcoma-associated ...
10
Tetrameric Ring Formation of Epstein-Barr Virus Polymerase Processivity Factor Is Crucial for Viral Replication
... polymerase processivity factor, which is essential for viral genome replication, exists mainly as a C-shaped head-to-head homodimer but partly forms a ring-shaped tetramer through tail-to-tail ...polymerase ...
10
The Flexible Loop of the Human Cytomegalovirus DNA Polymerase Processivity Factor ppUL44 Is Required for Efficient DNA Binding and Replication in Cells
... a processivity factor, including the ability to dimerize, as well as bind to, pUL54 and DNA, reside in the N-terminal portion (26, 45), whereas the C terminus is essen- tial for phosphorylation-regulated, ...
10
Cloning and Functional Analysis of Kaposi’s Sarcoma-Associated Herpesvirus DNA Polymerase and Its Processivity Factor
... and processivity factor, respectively. Processivity factors com- plex with DNA polymerases, allowing them to synthesize ex- tended stretches of DNA without dissociating from the tem- ...and ...
5
Phosphorylation of the Epstein-Barr Virus (EBV) DNA Polymerase Processivity Factor EA-D by the EBV-Encoded Protein Kinase and Effects of the l-Riboside Benzimidazole 1263W94
... the EBV DNA polymerase processivity factor, EA-D, during viral reactivation in latently EBV-infected Akata cells is analyzed. This pattern specifically changes with progression of cytolytic infection. In ...
6
Phosphorylation of Kaposi's Sarcoma-Associated Herpesvirus Processivity Factor ORF59 by a Viral Kinase Modulates Its Ability To Associate with RTA and oriLyt
... (EBV) processivity factor, is phosphorylated by the BGLF4 viral kinase within a hinge region- like domain known to be important for inducing conformational changes ...
15
The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques
... BioMed CentralVirology Journal ss Open AcceResearch The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in dif[.] ...
20
The Late Promoter of the Human Cytomegalovirus Viral DNA Polymerase Processivity Factor Has an Impact on Delayed Early and Late Viral Gene Products but Not on Viral DNA Synthesis
... Transcriptional regulation of UL44 gene expression occurs at two levels. The two transcription start sites that are activated early in infection presumably respond to IE proteins. Each contains a conventional TATA ...
10
The SWI/SNF Chromatin Regulator BRG1 Modulates the Transcriptional Regulatory Activity of the Epstein-Barr Virus DNA Polymerase Processivity Factor BMRF1
... ase processivity factor (BMRF1), helicase (BBLF4), primase (BSLF1), primase accessory proteins (BBLF2/BBLF3), and uracil DNA glycosylase (BKRF3) and replicates viral DNA in a manner similar to that of the ...
21
Mutations That Increase DNA Binding by the Processivity Factor of Herpes Simplex Virus Affect Virus Production and DNA Replication Fidelity
... The interactions of the herpes simplex virus processivity factor UL42 with the catalytic subunit of the viral polymerase (Pol) and DNA are critical for viral DNA replication. Previous studies, including one ...
8
cdc2 Cyclin-Dependent Kinase Binds and Phosphorylates Herpes Simplex Virus 1 UL42 DNA Synthesis Processivity Factor
... The studies conducted in the past several years have shown that HSV gene products scavenge the cell for cellular regula- tory proteins which may be stabilized and translocated to spe- cific compartments. In some ...
8
The extreme C terminus of herpes simplex virus DNA polymerase is crucial for functional interaction with processivity factor UL42 and for viral replication.
... We also tested the effect of the disruptive C-terminal mutations on UL42 binding when set in the background of full-length polymerase, because although AN960 bound UL42 with an efficienc[r] ...
9
The UmuC subunit of the E coli DNA polymerase V shows a unique interaction with the β clamp processivity factor
... Background: Strict regulation of replisome components is essential to ensure the accurate transmission of the genome to the next generation. The sliding clamp processivity factors play a central role in this ...
13
Differential Requirement of Human Cytomegalovirus UL112-113 Protein Isoforms for Viral Replication
... polymerase processivity factor pUL44 ...polymerase processivity factor pUL44 to intranuclear prereplication foci, suggesting that the re- cruitment of pUL44 is of crucial importance for viral ...
13
Epstein-Barr Virus-Encoded Protein Kinase (BGLF4) Is Involved in Production of Infectious Virus
... Effect of EBV PK knockdown on viral DNA synthesis. EBV PK has previously been shown to hyperphosphorylate viral DNA polymerase processivity factor EA-D (the BMRF1 gene FIG. 1. EBV BGLF4-targeting siRNA ...
6
Maribavir Inhibits Epstein-Barr Virus Transcription in Addition to Viral DNA Replication
... The second group of drugs includes quite different com- pounds with distinct structures, foremost among them being MBV and its parent, D -ribonucleoside (BDCRB), each of which has a completely different mode of action ...
10
Identification of Amino Acids That Are Critical to the Processivity Function of Respiratory Syncytial Virus M2-1 Protein
... transcriptional processivity factor for PVM tran- ...M2-1 processivity function can be mea- sured by using the RSVlacZ minigenome assay ...the processivity function of RSV M2-1 was required ...
8