protease domain
Characterization of the rubella virus nonstructural protease domain and its cleavage site.
... NS protease domain. The RUB NS protease domain and its cleav- age site have been shown by deletion mapping to be located within the domain composed of amino acids 1005 to 1507 ...
7
Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of the capsid protein from the polyprotein
... RSV protease as a template (Figure ...the protease domain is part of a multi-domain protein, additional interactions among the domains may influence the folding of individ- ual ...the ...
12
Determinants for Membrane Association of the Hepatitis C Virus NS2 Protease Domain
... In the monocistronic context, mutation K131A completely abolished virus production (Fig. 3A, left panel). This defect could be partially rescued by introduction of a lysine in position 134 (K131A/A134K). Immunoblot ...
5
Assembly and Processing of Human Immunodeficiency Virus Gag Mutants Containing a Partial Replacement of the Matrix Domain by the Viral Protease Domain
... catalytic Asp residue, is defective in Gag processing. The MA(p6ⴱ-PR)D25 mutant contains a deletion of 105 codons and a replacement of the p6ⴱ-PR coding sequence in the MA protein. The myristylation signal residues and a ...
5
Involvement of the N-Terminal Deubiquitinating Protease Domain of Human Cytomegalovirus UL48 Tegument Protein in Autoubiquitination, Virion Stability, and Virus Entry
... DUB domain adjacent to the NLS may affect the pUL48-associated activity in routing the capsid to the nuclear pore, which requires the func- tional NLS, as shown in a recent study using HSV-1 pUL36 ...DUB ...
14
Mutational Analysis of Stubble-stubbloid Gene Structure and Function in Drosophila Leg and Bristle Morphogenesis
... serine protease domain, a short N-terminal intracellular domain, and a variety of structural motifs connecting the transmembrane domain with the catalytic domain (H ooper et ...
17
Identification of a Ca2+-Binding Domain in the Rubella Virus Nonstructural Protease
... (M), protease (P), helicase (H), replicase (R), and a proline-rich region (G), as well as an X domain (X) showing high homology to the Appr-1-p processing enzyme (1, ...its protease domain ...
12
Mutational Analysis of the Caenorhabditis elegans Cell-Death Gene ced-3
... a protease similar to interleukin-1b converting enzyme and related proteins termed caspases, prevent programmed cell death in the nematode Caenorhabditis ...CED-3 protease and the aspartate residues at ...
17
An amino-terminal domain of the hepatitis C virus NS3 protease is essential for interaction with NS4A.
... the protease, as if covalent linkage were partially compensating a defective protein-protein interaction ...mutated protease, and therefore the N-terminally deleted protease, once released from the ...
9
Murine Coronavirus Ubiquitin-Like Domain Is Important for Papain-Like Protease Stability and Viral Pathogenesis
... papain-like protease by mutagenesis of the required catalytic cysteine residue or inhi- bition with a protease inhibitor blocks viral replication (23, 34, 35, ...for protease activity but represent ...
11
Biochemical Defects of Mutant nudel Alleles Causing Early Developmental Arrest or Dorsalization of the Drosophila Embryo
... serine protease domain that is specifically required for ventral ...the protease domain of ...the protease domain is required for dorsoventral patterning and that the Nudel ...
11
Influence of Coagulation Factor Zymogens on the Infectivity of Adenoviruses Pseudotyped with Fibers from Subgroup D
... (EGF)–EGF–serine protease domain structure on virus trans- duction, we transduced HepG2 cells with a subset of viruses, Ad5/f47, Ad5/f33, and Ad5/f30, in the presence of physiologi- cal concentrations of ...
5
Mapping of an Internal Protease Cleavage Site in the Ssy5p Component of the Amino Acid Sensor of Saccharomyces cerevisiae and Functional Characterization of the Resulting Pro- and Protease Domains by Gain-of-Function Genetics
... serine protease that activates the transcription factor Stp1p upon exposure of the yeast to extracellular amino ...the protease domain of Ssy5p exhibited the decreased 50% effective concentration (EC ...
8
Mutagenesis of the NS3 Protease of Dengue Virus Type 2
... the protease activities of the parent and wt constructs, six additional chimeric plasmids, a to f, were gen- erated by swapping relevant restriction fragments ...2A). Protease activity was analyzed in vivo ...
9
Evolution of the initiating enzymes of the complement system
... of domain as MASP-3-like - whereas MASP-1, which uses the downstream serine-protease-encoding region of the MASP- 1/3 gene, has a more typical serine-protease ...
5
Structure of the Dengue Virus Helicase/Nucleoside Triphosphatase Catalytic Domain at a Resolution of 2.4 Å
... cluding the DNA helicases PcrA from Bacillus stearother- mophilus (39) and Rep from Escherichia coli (25) that are representatives of superfamily 1 and the RNA helicase from HCV (48) and UvrB, an enzyme involved in ...
11
The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones
... Two genes of particular interest to the study were the serine protease inhibitor serpin cd00172, originally targeted to select this BAC sequence, and the large multiple domain papilin ge[r] ...
16
The role of APP in Alzheimer’s disease
... extracellular domain of APP that contains two heparin- binding regions ...extracellular domain was shown to act as a ligand for Death Receptor 6, thus activating caspase 3- triggered cell body apoptotic ...
6
Foamy Retrovirus Integrase Contains a Pol Dimerization Domain Required for Protease Activation
... by light scattering. (A) Purified recombinant PR-RT was prepared and loaded onto a 1.0-cm by 30-cm Superdex 200 column connected to an Optilab DSP interferometric refractometer followed by a Dawn EOS multiangle light ...
7
Mapping of a Hypovirus p29 Protease Symptom Determinant Domain with Sequence Similarity to Potyvirus HC-Pro Protease
... Viability of progressively repaired ⌬ p29 recombinant vi- ruses. In an effort to map the determinants within p29 that contribute to CHV1-EP713-mediated suppression of fungal pigmentation and asexual sporulation, the ⌬p29 ...
7